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The determination of cytokinin levels is a primary and basic approach in the cytokinin studies. The Amaranthus betacyanin bioassay was modified for the rapid and convenient determination of cytokinin activity. Meanwhile, the procedures for quantitative analyses of cytokinins by HPLC and ELISA were estabolished, and these gurantee the analyses of cytokinin levels with high precision.

在细胞分裂素的活性测定中,通过改进尾穗苋苋红素合成法建立了一种简便、准确的生物试法,同时还建立了根据物理化学和免疫学原理而测定细胞分裂素的HPLC和ELISA方法,使得细胞分裂素的定量更加准确。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜,采有用酶消化法和组织培养法获取成骨细胞体外培养并传代,观察细胞形态,生物特点及成骨特性,并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径,将不同浓度的钛合金颗粒(1mg/ml,0.1mg/ml,0.01mg/ml)与成骨细胞共同培养,分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml,0.1mg/ml,0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

Even more, since it has the active substance of natural berbal medicine, whiceh can permeate into the conified layer of skin, it can clean the detrimental substances, and kill the bacteric and virus.

它含有昆达---2号生物淬取液和名贵香料、维生素、天然保湿分子、蜂王浆等多种营养成份,使其不仅具有气味清新、泡沫丰富、润肤养颜等特点,更因其含有天然药用植物的活性物质,可深入人体皮肤角质层,清除有害物质,杀灭病菌,对宫颈糜烂有显著的辅助疗效、对由真菌引起的脚气、湿疹、体等皮肤病具有奇特的治疗效果。

Thus explores the process parameter of aerated biological ceramicite filter to use in the film waste water, using this system to substitute traditional process of active sludge processing film waste water, make treated sewage reach index of retrieval and utilization.

从而探索出曝气生物滤池用于胶片废水处理的工艺参数,通过此工艺取代传统的活性污泥法处理胶片废水的工艺,使处理后的污水达工业回用水的指标。

The pearl is unique, the only gemstone that grows in a living organism. Peals are in a position of antioxidant ability and hence consenescence can be retarded.

珍珠是世界上独一无二的在生物体内生长出来的宝石,她的有效活性成分有水解氨基酸、微量元素、牛磺酸及钙离子,能抵抗氧化作用及清除活性氧自由基的能力,具有防衰老的功能。

Another remarkable characteristic of printing and dye the waste water is that the colour is high, is it adopt biological carbon pool is it is it deal with to decolour to go on to it , for activated carbon absorbent spent to design originally, active charcoal adsorption method to utilize porous active charcoal material make one or many kinds of material of waste water it absorbs to be that surface get rid of in active charcoal.

印染废水的另一显著特点就是色度高,本设计采用生物碳池对其进行脱色处理,所用的吸附剂为活性碳,活性炭吸附法就是利用多孔活性炭物质使废水中的一种或者多种物质被吸附在活性炭表面而去除的。

Ergothioneine is a kind of precious amino acid, and it is also a natural antioxidant. It plays an important role to prevent the oxidative damage in organism. Ergothioneine cannot be biosynthesized in humans, only can be absorbed from the diet. In recently the research discovered the high content of ergothioneine in the mushroom. Content of ergothioneine of Pleurotus eryngii is the highest from 28 kinds of mushrooms, therefore the research untilized P. eryngii to make the products of P. eryngii-fermented adlay and P. eryngii-fermented buckwheat which content high ergothioneine. Also, we analyzed their proximate composition, nutritional components, taste and physiologically active components, antioxidant properties and evaluation of anti-imflammatory effect.

中文摘要麦角硫因为一种稀有的胺基酸,也是天然的抗氧化剂,其在生物体内对氧化压力伤害之预防扮演著重要角色,麦角硫因不能在动物体内合成,仅能由食物中摄取,近年来发现在菇类中含有高含量的麦角硫因,从28种食药用菇类中筛选出麦角硫因含量较高的菌种,以杏鲍菇子实体最高(1521.6 mg/g dw),因此本论文以杏鲍菇为媒介,利用固态发酵方式,制备出高麦角硫因之杏鲍菇薏仁和杏鲍菇荞麦,并针对薏仁、杏鲍菇薏仁、荞麦、杏鲍菇荞麦和杏鲍菇菌丝体,进行一般成分、营养、物性、呈味、生理活性物质、抗氧化性质之分析及抗发炎反应之评估。

The results of bioassay experiments with 10 commonly used fungi showed that, all the title compounds had satisfactory fungicidal activity against Botrytis cinerea, the growth inhibition rate of compounds 5ae, 5ag, 5bd against Botrytis cinerea were up to 70% and for 5ad, 5cd, 5ce were more than 60%; compound 5bd exhibited fungicidal activity against Pellicularia sasakii with growth inhibition up to 60%, the growth inhibition rate of compound 5cg against Colletotrichum lagenarium was 69%.

抑菌活性生物测定结果表明,合成的大部分化合物对黄瓜灰霉病菌均有较好的生长抑制作用,其中,化合物5ae、5ag和5bd的抑制率达到70%;化合物5ad、5cd、5ce的抑制率大于60%;5bd对水稻纹枯病菌抑制率大于60%,化合物5cg对西瓜炭疽病菌的抑制率为69%。

The mechanisms of simultaneous nitrification and denitrification,short-cut nitrification and denitrification,and denitrifying phosphorus removal are discussed.

膜生物反应通过膜的高效截留作用,可维持反应器内高浓度的生物量,满足硝化菌的生长增殖需求,强化活性污泥的硝化能力。

Methods 32 rats were euthanized and femurs were removed by disarticulated. The left femurs were heated for 3 minutes, 5 minutes, 10 minutes and 15 minutes with the microwave and the histochemical assay for special lactate dehydrogenase activity was evaluated. The rat bone's biomechanics and the content of calcium tests were carried out.

应用 微波对实验组股骨分别加热3、5、10、15min后,用特殊的乳酸脱氢酶活性染色,判断骨、软骨细胞的活力;用Instron万能材料试验机测定股骨的生物力学并同步记录;用原子吸收分光光度仪测定股骨钙的含量。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。