生物活性的

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

Mimetic peroxidase of high catalytical activity and selectively is very important in bio-analytical field.

建立一系列关于这些酶的模拟体系将为以下三个方面的研究工作提供非常重要的工具：（1）工业或者精细化工方面重要反应新型催化剂的开发，例如烷烃的选择性氧化以及具有立体选择性的烯烃环氧化；（2）新的生物活性物质，例如药物的氧化机理的研究和预测；（3）具有合适底物的高活性过氧化物酶模拟酶在生化分析领域的前景。

Expanding pinacol reductive coupling reaction into asymmetric one with both diastereoselectivity and enantioselectivity may lead to its application in synthesising medicaments and biologic active compounds with structure of vicinal diol. Asymmetric pinacol coupling reaction is also the most compact and effecient method in synthesizing chiral ligands or chiral auxiliary with C〓 symmetry.

将这一反应发展成为既有高度的非对映选择性，又有高度对映选择性的不对称偶联反应在合成许多含有手性邻二醇结构的药物或生物活性物质将有更重要的应用，同时不对称Piancol偶联反应也是合成C〓对称性的邻二醇多用途手性配体或手性助剂的最为简捷有效的方法。

Group of Yale undergraduates have discovered dozens of potentially beneficial bioactive microorganisms within plants they collected in the Amazon rain forest, including several so genetically distinct that they may be the first members of new taxonomical genera.

组的耶鲁大学的本科生已发现几十个潜在的有益微生物的生物活性植物，他们收集的亚马逊雨林，其中包括一些独特的基因，使他们可能成为第一个成员的新分类属。

Fluouricil is usually used as anticancer drug, its derivative, 5-fluoro-uracil-1- acetic acid has good biological activity. Its complexes were presented in literatures. However, the coordination modes of it are paradox in literatures. The single crystal structure was resolved to clarify the confusion. The result indicates the carboxylic group coordinates to zinc ion in unidentate, and the heteroatoms of pyrimidine ring are not involved in coordination. Antitumor activity against SMMC-7721 was also examined.

7.5-氟尿嘧啶是临床上广泛使用的抗癌药物，它的衍生物5-氟尿嘧啶-1-乙酸亦具有良好的生物活性，有关它的金属配合物亦有报道，但其结构指认相当混乱，为此研究了它与锌的配合物单晶结构，证明了5-氟尿嘧啶以单齿羧基与锌配位，而嘧啶环氮并未配位，也研究了它对SMMC-7721细胞株的抗肿瘤作用。

MethodsThe activity of macrophage cell in abdomen cavity and NK cell in spleen and the SIgA contents in saliva were detected, and the survival rate of mice which had been infected by influenza virus FM 1 were observed after the powder of Le IFN α/supplement material mixture had been insufflated in mice buccal cavity.

一些研究表明，人体的干扰素受体，主要分布在口腔、腭咽及鼻腔黏膜部，只要少量的IFN同上述部位的受体结合，即可发挥IFN的生物活性[1] ，同时也有不少以人的αIFN通过口腔途径给药用于抗病毒和免疫调节的动物实验[2 ] 。

Biological activities of SPThe inhibitory effect of SP on oxidation of lipids and the acitivity of lipoxygenase, the scavenging or quenching effect of SP on free radicals, and the influences of SP on the malonaldehyde content in mice liver juice, liver mitochondria and liver red blood cell were studied. The results showed that SP can inhibit the auto-oxidation of lard and soybean oil at 60 and can inhibit the activity of lipoxygenase significantly; SP also had scavenging effect on hydroxylradicals generated by Fenton system with IC50 value 6.220 mg/ml, under the concentration range of 1 mg/ml-50mg/ml, the scavenging rate of SP to -OH increased strongly when the concentration of SP increased, the maximum scavenging rate was 85.4%.

大豆多肽的生物活性研究研究了大豆多肽抗油脂氧化、抑制脂肪氧合酶及清除自由基的作用，以及对小鼠肝组织匀浆、肝线粒体和红细胞丙二醛含量的影响，结果显示：在60℃时，大豆多肽对猪油与豆油的自动氧化均有一定的抑制作用：对脂肪氧合酶的抑制作用显著：对Fenton体系产生的羟基自由基具有明显的清除作用，其IC_(50)值为6.220mg／ml。

PL could maintain cellular membrane integrity,induce the oxidative damage to biomembrane by free radicle and improve antioxygen ability and ATPase activity of larval fish.5 Effect of phospholipid supplementation in formulated microdiets on foregut and liver histological changes of Pelteobagrus fulvidraco larvaePurpose:This study was undertaken to determine the effect of commercial PL supplementation in formulated microdiets on foregut and liver histological changes of Pelteobagrus fulvidraco larvae,which can further investigated the mechanism of the growth-promoting effect of PL with the study of histology and digestive physicalogy.

磷脂能够维持黄颡鱼仔稚鱼细胞膜的完整性，降低自由基对生物膜的过氧化损伤，提高机体抗氧化系统的活力和ATP酶活性。5饲料中不同磷脂添加水平对黄颡鱼仔稚鱼前肠和肝脏组织学的影响目的：研究在实际配方的人工微粒饲料中添加商品大豆磷脂对黄颡鱼仔稚鱼消化肠道肝脏器官发育及脂肪消化吸收的影响，进一步从组织学消化生理角度揭示磷脂对仔稚鱼促生长作用机理。

Research discover dogwood A has the good protective function to the CCl4 liver damage model and the blood vessel endothelial cell, therefore think that A is in the dogwood an important biological active ingredient, and still discover that the dogwood liquor steamed product A content increase obviously to living product, confirm the steamed system can increase A content; Moreover, discover living product water decoction A content have four times as much as methanol supersonic extraction, but made product difference is not big, this also confirm the heating really has very big influence to A content, but do not further study the dogwood the living product and the liquor steamed product decoction change in body content.

研究发现山茱萸中A对CCl4 肝损伤模型及血管内皮细胞具有良好的保护作用，因此认为A为山茱萸中一重要的生物活性成分，而且还发现山茱萸酒蒸品中A的含量较生品中明显增加，证实了蒸制能增加A的含量；另外，发现生品水煎煮提取物中A的含量也较甲醇超声提取物中的高出约3倍，而制品则差别不大，这也证实了加热对A的含量确有很大的影响，但没进一步研究山茱萸生品与酒蒸品水煎液在体内的含量的变化。

And some traditional metal materials, non-metallic materials such as steel, cement, plastics, fibers and rubber has been widely used, and a new type of polymer materials is also a beginner's stage, but the very rapid development with very potential, and its positive features, intelligence, refinement of direction, conductive materials, energy storage materials, smart materials, nano materials, optical materials, biological activity, such as research in the field of materials are becoming increasingly active, the study of Polymer Materials very challenging, but also very innovative test of their own.

并且目前一些传统的金属材料，非金属材料如钢铁、水泥、塑料，纤维，橡胶等已经得到了广泛的应用，而新型的高分子材料还在一个起步的阶段，但是发展的很迅猛，具有很大的潜力，其正向功能化，智能化，精细化方向发展，导电材料，贮能材料，智能材料，纳米材料，光导材料，生物活性材料等领域的研究也日趋活跃，所以研究高分子材料很具有挑战性，同时也很考验自己的创新性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。