生物活性的

Brachiation along the branches of such trees from structurally complex to simple scaffolds with retained yet varying bioactivity is feasible at high frequency for the five major pharmaceutically relevant target classes and allows for the identification of new inhibitor types for a given target.

结构上从复杂到简单的支架的树分枝而仍旧保留不同的生物活性的臂力摆荡，就五大主要药学相关靶分级而言是可行的，并且允许新的特定目标类型抑制剂识别。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

At the same time, increasing the negative charge of O at C7 and N at thiazole in C15 sidechain is favorable.

同时，C7上的O原子和C15侧链噻唑基团上的N原子的负电荷的增加，对epothilones的生物活性的增大也是有利的。

The epidemiological investigation of the field suggested that there may have an infectious virus unit at least, in 96L air by threshing machine and 350L air by the nest of A. agrarius , respectively.

对现场采集的空气样品和收集的打谷者佩戴的口罩样品的研究发现，在稻田堆放的稻捆根部和鼠栖息的草窝的空气中每 35 0L空气中和打谷场脱粒机附近每 96L空气中，含有至少一个具有生物活性的汉坦病毒粒子。

Imino-1, 3-thiazetidines is the basic structure of a kind ofbioactive herbicide and insecticide. Our method has advantage in synthesis highly substituted2-imino-1, 3-thiazetidines, therefore it has practical value to some extent. We also researched the reactions catalyze by active zinc dust.

提供了成四员噻吖丁啶杂环衍生物的方法。2-亚胺基-1，3-噻吖丁啶是一类具有生物活性的除草剂及杀虫剂的核心结构，我们的方法在合成多取代的2-亚胺基-1，3-噻吖丁啶方面有一定的优越性，从而具有一定的实用价值本文另一部分的工作是探讨锌粉催化的反应。

In the present project,the project group investigated Cololejeunea flora of some provinces of China, collected over 500 specimens of Cololejeunea and over 7000 specimens of other liverworts,and borrowed over 3500 specimens （including 416 types）from other important herbaria. The species diversity and distribution weree clarified preliminary by means of mophologocal and ecologoical observation and studies on oil bodies and chromosomes as well as bioactivity of some species.

本项目对我国部分省区作了详尽的野外考察，采集了500余份我国疣鳞苔属的标本和7000多号其它苔类植物标本，从世界各大标本馆借阅了3500余份标本（包括416份模式标本），通过形态学，解剖学和生态学的观察，结合一些种类的染色体和油体以及生物活性的研究，初步搞清了我国和其它亚洲国家疣鳞苔属的种类和分布。

Quercetin, quercetin rhamnoside, hyperin, syringoside and eleutheroside E wereisolated from the flower of Acanthopanax senticosus. Searching bioactive natural compound and establishing quality evaluation standard arefocused on this experiment. Using cold immersing of 70% methanol to extract from flower ofAcanthopanax senticosus and integrating macroporous resin, silica gel column chromatography,sephadex gel to abstract. We obtained five monomer compounds (compoundⅠ-compoundⅤ),quercetin, quercetin rhamnoside, hyperin, syringoside and eleutheroside E. That five compoundswere determined by HPLC in eight parts of Acanthopanax senticosus (flower, root, leaf fruit,shoot, new shoot, phloem of shoot, skin of shoot), which provide science bases for establishingquality evaluation system and settle infarctate foundation.

本文以从刺五加花蕾中寻找具有重要生物活性的天然化合物和建立质量评价标准为主要目的，对其化学成分进行研究，采用70％甲醇冷浸的方法对刺五加花蕾中的成分进行提取，运用大孔树脂吸附色谱法、硅胶柱层析法以及葡聚糖凝胶柱层析相结合的方法进行分离，得到5种单体化合物，分别为槲皮素、槲皮素鼠李糖苷、金丝桃苷、紫丁香苷和刺五加苷E；利用高效液相色谱法对这5种化合物在刺五加花蕾中的含量进行了测定；考察了8个部位(花蕾、根、叶、果、茎干、新枝、茎韧皮部、茎皮)中这5种化合物的含量分布情况，并作一比较，为今后建立刺五加质量评价体系提供了科学依据，奠定了坚实的基础。

Progresses on reactions of NO, CAN, and hydrazone Reactions of NO with olefines, phenols, amines, arylhydrazines, Schiff bases and nucleosides are firstly reviewed.

本论文主要研究了一氧化氮及硝酸铈铵与简单醛酮腙、α，β-不饱和酮腙的反应，全文共分五个部分： 1、一氧化氮、硝酸铈铵及腙的反应研究首先对具有特殊生物活性的NO与烯、酚、胺、肼、Schiff碱、核酸碱基等有机物的反应做了全面的总结。

The aim of this paper is to identify the effect of heavy metals on wheat root's growing up and their biological activities in soil with different fertilizer conditions were investigated.

本文的研究目的在于了解土壤重金属污染对小麦根部生长发育的影响以及在不同培肥条件下土壤重金属的生物活性的变化情况。

However, due to seasonal and geographic differences, the natural lysate of horseshoe crab hemocytes suffers the disadvantage of batch-to-batch variation in its sensitivity of detection of endotoxin.

因而，为了获得大量可用于研究与临床治疗的TALF和FC蛋白，只有利用基因工程的手段才有可能大量制备具有生物活性的重组蛋白以满足应用的需要。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。