生物活性的

On the basis of this system andpreviously morphological, physiological and biochemical studies on cucumber in vitrogynogenesis. Materials from both typical gynogenesis using M99 as induction medium andnon gynogenesis using W5 as induction medium were collected. The activities anddistribution of peroxidase and its isoenzymes, content of soluble protein and distribution of allprotein during early stage of cucumber in vitro gynogenesis and non gynogenesis wereinvestigated by biochemistry and histochemistry technology.

本试验在前期有关离体黄瓜雌核发育形态学和生理生化研究的基础上，以诱导离体雌核发育的 M99 培养基上和非雌核发育的 W5 培养基上培养的黄瓜未受精子房为试材，采用生物化学技术和组织化学技术，深入研究了离体雌核发育早期外植体内的标志酶——过氧化物酶及其同工酶的活性及分布的变化、可溶性蛋白质的含量和总蛋白质分布的变化及核酸和多糖分布的变化，为揭示离体雌核发育早期的细胞分化机制提供科学依据。

Give birth to content and food engineering according to university of industry of project technology chief, Dalian courtyard dean Zhu Bei osmund professor introduction, this project developed microtherm vacuum to permeate first flavor to style of technology, stage warms up and cool antiseptic processing technique, in the treatment that uses at eating abalone product namely, solved original feed the difficult problem of technology of not easy pilot such as abalone product appearance, mouthfeel, aroma, color namely, built the manufacturing technology system that feeds abalone namely, to develop other ocean shellfish fed a product to lay good foundation namely; Use first from dissolve enzymatic with outside polysaccharide of abalone of extraction of method of source enzymatic compound, raised the recovery rate of polysaccharide greatly; Use enzymatic law and the method that Sevag law photograph combines, solved effectively take off the difficult problem of miscellaneous albumen in dividing abalone polysaccharide, raised polysaccharide purity, maintained polysaccharide active, the technical system of the preparation that built abalone polysaccharide, detached, rarefied, analysis, draw lessons from goodly to developed polysaccharide of other and marine animal to offer.

据项目技术负责人、大连工业大学生物与食品工程学院院长朱蓓薇教授介绍，该项目首次开发了低温真空渗透调味技术、阶段式升暖和冷却杀菌加工技术，并应用于即食鲍鱼产品的加工中，解决了原有即食鲍鱼产品外形、口感、香气、颜色等不易控制的技术难题，建立了即食鲍鱼的生产技术体系，为开发其他海洋贝类即食产品奠定了良好的基础；首次采用自溶酶与外源酶复合的方法提取鲍鱼多糖，大大提高了多糖的提取率；采用酶法与Sevag法相结合的方法，有效地解决了脱除鲍鱼多糖中杂蛋白的难题，提高了多糖纯度，并保持了多糖活性，建立起了鲍鱼多糖的制备、分离、纯化、分析的技术体系，为开发其他海洋动物多糖提供了良好的借鉴。

Which can be used as the body of vitamin B1 coenzyme of many enzymes in the normal human body has an important role in glucose metabolism, it can inhibit the activity of cholinesterase, and choline esterase is an important neurotransmitter, and thus nerve conduction impact; vitamin B2 in the organization by participating in a variety of yellow enzymes constitute a coenzyme, its role in bio-oxidation process in the delivery of hydrogen role in regulating the body's metabolism process; vitamin B6 can also be a variety of enzymes as coenzyme participate in all kinds of amino acid metabolism, promote the absorption of amino acids and protein synthesis, cell growth is necessary for the material, which also promote fat metabolism, involved in neurotransmitter synthesis, hemoglobin synthesis and the impact on the normal reproductive functions such as human body an important metabolic process, Volkswagen Medicine Net: What liver protection effect of vitamin B family?

可作为维生素乙许多酶的辅酶，在正常人体组织中有一个用糖代谢的重要作用，能抑制胆碱酯酶活性，和胆碱脂酶是一个重要的神经递质，神经传导，从而影响；由黄色参与各种酶在组织中维生素B2构成辅酶，其作用在生物氧化氢的作用，调节人体的代谢过程中产程；维生素B6也可以是作为辅酶参与氨基酸代谢的各种酶的品种，促进氨基酸的吸收和蛋白质合成，细胞生长是必要的材料，这也促进脂肪代谢的神经递质合成，血红蛋白合成和参与，如影响人体生殖功能的正常新陈代谢过程中一个重要的，（大众医药网：什么样的维生素B家庭肝脏的保护作用？

Naturally occurring terpenes constitute the largest group of natural products with complicated and variable structures and a great number of important biological activities.2-Methyl-D-erythreitol 4-phosphate pathway is a newly found and established biosynthetic route for terpenoids and all the enzymes involved in this pathway can be used as targets for the screening of new antibiotics.

萜类化合物构成了最大的一个天然产物家族，结构复杂多变，且有许多重要的生理活性。2-甲基-D-赤藓糖醇4-磷酸途径是近年来发现并建立的一条萜类化合物的生物合成途径，其中所涉及到的酶均可作为靶标来进行新抗菌素的筛选。

The compost of agricultural waste materials is the process that under the function of microorganism, the organic matter turn into well-rotted manure by the way of the organic matter being mineralized and humus and harmless.

农业废弃物的堆腐是在微生物作用下，通过发酵使有机物矿质化、腐殖化和无害化而变成腐熟肥料的过程，在微生物分解有机物的过程中，不但生成大量可被植物吸收利用的有效态氮、磷、钾化合物，而且又合成新的高分子有机物---腐殖质。由于这一过程是在微生物分泌酶的作用下酶促反应过程，研究酶活性变化可以从本质上揭示堆腐的生物行为。

By suspending cell culture from the callus of P. thugbergii and by fluorescent observation, a new method, which is easy and sensitive to observe cell activity and to test the phytotoxicity of various bacteria, was used. This method can be utilized to study the bacterial toxins. Cultured filtrates of some strains of the nematode-carrying bacteria were bioassayed for their toxicity to suspending cultured cells from P. thugbergii callus. Strong toxic effects were observed by the filtrates of Pseudomonas spp., P. fluorescence, and Sphingomonas spp., these bacteria species are coincide with those in the early stage of pine wilt disease.

本试验使用了黑松愈伤组织的细胞悬浮培养方法，用荧光方法观察各种细菌对植物细胞的毒性测定，这种方法具对细胞的活性观察容易、灵敏度高等特点，证明可用于细菌的毒素研究；对松材线虫携带的细菌的部分菌株，用其培养滤液对黑松愈伤组织的悬浮培养细胞的生物测定初步证明，松材线虫携带的细菌中以假单胞菌、荧光假单胞菌和少动鞘氨醇单胞菌的滤液对黑松细胞具有较强的毒性，这与松树发病早期出现的细菌种类相一致。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。