生物样品

The enzymatic digestibility of native and acetated tapioca starches with different degree of substitution was investigated in in-vitro digest model stimulated the human intestine tract and the resistant starch content was determined using Megazyme total starch assay kit.

采用生物体外消化模型模拟人体消化道环境，对不同取代度的醋酸酯化木薯淀粉的消化速率进行了研究；用微生物酶对醋酸酯化木薯淀粉进行生物降解并测定各个样品的抗消化淀粉含量。

The visualization biological chip in this invention can make the sample simultaneously accept the high efficiency laser source and uses CCD cameral select the fluorescence image of the biological chip rapidly with large area for further analysis.

本发明的显像式生物芯片仪利用准直透镜使镭射光源产生大面积的镭射光激发光源，使得各样品点同时接受高效能的激发光源，此外利用CCD相机大面积而快速的选取生物芯片的荧光影像再进行分析，可以大幅减少分析时间。

Thus, it is helpful to understand mutual reactions among pollutants in heavy metal polluted soil and eutrophic water, to clarify their biological and environmental effect through studying phototactic behavior of Daphnia influenced by nitrogen, phosphate and heavy metals.

因此，戮们筛选培育的隆线搔纯生物株是一种非常理想的生物监测器，优于国外培育使用的大型搔，可以根据隆线搔的趋光行为的变化来监测样品中的有害物质。②在水搔趋光指数测定过程中，测定装置的工作条件和参数显著影响结果。

These indicates bioactive peptides in Chinese forest frog skins have great potential for the exploitation and utilization. S_(180) cells were inoculated hypodermically at the concentration of 1×10~6 cells per mouse and the bioactive peptides in Chinese forest frog skins were resolved into aseptically distilled water to make injection liquid. After injected continuously 15 days, the inhibition rate of tumor was calculated. The induction of tumor cell apoptosis of active peptides in Chinese forest frog skins could be detected by morphological

将S_(180)胃肉瘤细胞接种至小鼠皮下，选取中国林蛙皮肤生物活性肽不同浓度样品液，连续注射种瘤小鼠15天后，计算抑瘤率；采用透射电镜观察细胞形态学变化、DNA梯度琼脂糖凝胶电泳技术、MTT比色法、流式细胞检测等技术观察中国林蛙皮肤生物活性肽对K562肿瘤细胞凋亡过程的诱导。

For quantitatie real-time PCR of DR4 and DR5 transcripts, triplicated samples containing 9 l of cDNA with 10 l of Taqman Uniersal PCR Master Mix (Applied Biosystems, Foster City, CA) and 1 l of 20xAssays-on-demand Gene Expression Product (DR4; Hs 00269492_m1, DR5; Hs 00366272_m1, Applied Biosystems) were pre-incubated at 50oC for 2 minutes and subsequently at 95oC for 10 minutes.

对于进行定量实时PCR的DR4 和 DR5转录产物，使用10L 美国应用生物系统公司生产的 Taqman Uniersal PCR Master Mix试剂，1L的20xAssays-on-demand Gene Expression Product (美国应用生物系统公司生产的DR4； Hs 00269492_m1， DR5； Hs 00366272_m1)，先将其在50度下孵化2分钟，然后在95度下孵化10分钟，用这两种试剂将含有9L cDNA的样品扩增两倍。

HDL reference, in which 1μg apoAI was thought of as one activity unit, was prepared by ultracentrifuging.

测定时，以超速离心制备的HDL参考品为标准，按每1μg apoAI为1个HDL活性单位，由参考品的光吸收值和相应的活性单位数，求出HDL生物活性标准曲线的双对数回归方程，并由样品的光吸收值计算HDL制剂的生物活性。

Seven biogenic amines were extracted from a sample with 6% perchloric acid, then derivatized with benzoyl chloride. 0.02 mol/L borate buffer (pH 9.2) containing 0.06 mol/L sodium deoxycholate-methanol （95∶5, v/v）was used as a running buffer. The detection wavelength was set at 214 nm. The separation was achieved within 12 min. Good linearities were observed from the calibration plots in the concentration ranges.

样品经6%过氯酸萃取后，由苯甲酰氯衍生化，以含0.06 mol/L脱氧胆酸钠的0.02 mol/L硼酸(pH 9.2)-甲醇(体积比为95∶5)混合液为电泳介质，电泳电压25 kV，温度25 ℃，检测波长214 nm，在12 min内实现了7种生物胺的完全分离。7种生物胺的浓度与其峰面积在一定的范围呈良好的线性关系，检出限除组胺为15 μg/g外，其余均为5 μg/g。

In reference to ASTM water accommodation friction for preparing ecotoxic testing sample solution,the ecotoxicity of four kinds of nanometal additives were inspected and evaluated by applying luminescent bacteria .

采用发光细菌毒性测试技术，参照ASTM的WAF方法制备油类生物毒性测试样品液，对Cu、A、lZn、Ti 4种纳米金属添加剂的生物毒性进行了检测与评价。

SPR technique combined with the methods of material spreading onto the surface of gold developed in this work, including the polymer spreading by centrifugation and lipid spreading by LB technique, provided us a very sensitive tool for the real-time, dynamic and in-situ study of protein adsorption on the surface of biomaterials.

表面等离激元技术与本文采用的在金膜上铺展高分子材料的离心铺膜法和LB技术等样品制备技术相结合，为生物材料表面对蛋白质吸附特性的实时、动态、原位研究提供了一种新的高灵敏度的方法，并可能发展成为一种材料生物相容性的测试和评价的新方法。

SPR technique combined with the methods of material spreading onto the surface of gold developed in this work, including the polymer spreading by centrifugation and lipid spreading by LB technique, provided us a very sensitive tool for the real-time, dynamic and in-situ study of protein adsorption on the surface of biomaterials.

表面等离激元技术与本文采用的在金膜上铺展高分子材料的离心铺膜法和LB技术等样品制备技术相结合，为生物材料表面对蛋白质偏瘫吸附特性的实时、动态、原位祛斑研究提供了一种新的高灵敏度的方法，并腹泻可能发展成为一种材料生物相容性的测试瘦身和评价的新方法。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐