生物技术的

Directed molecular evolution of enzyme, originated in the beginning of the nineties of the last century, was a new strategy of protein engineering, which could rapidly modify proteins for specific purpose through imitating externally natural evolutions, as well as it has arisen a revolution in biocatalyst field.

酶的分子定向进化是20世纪90年代初兴起的一种蛋白质工程的新策略，是一种在生物体外模拟自然进化过程的、具有一定目的性的快速改造蛋白质的方法。该方法引起了生物催化技术领域的又一次革命。

Beijing East provide a series of service for purifying and desalting water on residential building, civicism, commercial and industrial activity etc.

东方海川为住宅、市政、商业与工业用水的净化和淡化提供一系列的技术服务，东方海川的技术服务涵盖的行业领域广泛，包括化学工业，食品工业，半导体和微电子工业，电力工业，生物制药工业等领域。

On the basis of MEBT, a new technique hypothesis of injecting MEBO under the eschar was proposed to have 6 biochemical reactions available, such as hydrolysis, enzymolysis, saponification, rancidify, lipofication, esterification of MEBO with necrotic tissue of the eschar, then to realize liquefaction and quick defluxion of necrotic tissues, This finally to play a role of regenerative action of MEBO in situ to turn potential regenerative cells into skin stem cells that would further proliferate and differentiate to form skin tissues, reaching the goal of physiologically repairing wounds.

最后提出了在烧伤湿性医疗技术正规治疗的基础上，同时进行痂下注射MEBO的新技术设想，其目的是使痂下组织尽早接受MEBO的治疗，痂下发挥MEBO的水解、酶解、皂化、酸败、脂化、酯化六大生物化学反应，促使坏死组织痂下痂外一起液化，尽快脱落；而且在痂下发挥MEBO原位再生作用，使潜能细胞转变成皮肤干细胞，干细胞进一步增殖分化形成皮肤组织，达到创面生理性修复之目的。

And gene engineering used in the store and innovation of germ plasm.

指定学生课外自学内容，适当介绍生物化学、分子生物学等最新技术在种子检验及品质分析上的应用，组织培养、基因工程技术在种质保存及创造上的最新进展，扩大课堂教学的信息量。

Combined with animal model of peradaptation to myocardium ischemia , the research observed the level of enzymatic dynamics of myocardium, SOD,MDA,ET and correlation peptide of calcitonin gene in peripheral blood and cardiac function, apoptic cell and expression of apoptosis regulatory gene, ultrastructure of myocardium with BIOPAC16, in situ end labeling and hybridism, optic microscope and electron microscope. The study of animal model of peradaptation to myocardium ischemia illustrated that the peradaptation and pretreatment with Wenxin capsule could be positively protective for the injury of ischemia reperfusion. The protection is mainly reflected by reduction of infarction area, correction of heart rhythm abnormality of ischemia reperfusion, melioration of the contraction and relaxation function of myocardium, recovery of the subcellular structure and themelioration of the abnormality of biochemical and bioactive substance in the myocardium.

并结合心肌缺血预适应动物模型，采用BIOPAC16导生理记录和分析处理系统处理心肌功能指标，采用原位末端标记及原位杂交技术标记凋亡细胞及凋亡相关调控基因的表达，采用光电镜技术观察心肌超微结构的变化，同时观察外周血心肌酶、超氧化物歧化酶、丙二醛、内皮素、降钙素基因相关肽等水平，结果表明心肌缺血预适应及温心胶囊预处理对心肌缺血再灌注损伤具有肯定的保护作用，其保护心脏作用主要表现在缩小心肌梗死范围、抗缺血再灌注心律失常、改善心肌收缩和舒张功能、减轻心肌超微结构损伤，调节心肌生化物质及生物活性物质的生成和释放。

Due to the uniqueness and invariability of fingerprint ,automatic fingerprint verification technology became the earliest and most widely used biometric authentication technology.

指纹的终身稳定性和唯一性使指纹自动识别技术成为最早和最广泛的被应用于实践的生物识别技术。

As the new medication-adjuvant,liposome has been widely applied in medication technology.

尽管脂质体这种新技术在生物领域内具有极其广阔的应用前景，但脂质体在该领域的研究应用中存在着较多的技术难题。

Since LIGA process is widely used in MEMS technology, Deep lithography is investigated in recent years.

随着LIGA技术在微电子机械系统加工工艺中的广泛应用，尤其是在生物科学技术领域的广泛应用，深度光刻技术也越来越成为研究应用的热点。

DESCRIPTION】the ingredients of the product comes from abyssopelagic fishes of special pure and clean sea areas, adopting Limited Enzymic Degraded Technology, a self-owned intellectual property, that is using compound bioenzyme preparations at a low temperature to extract active ingredients through a special low-temperature extraction process and specific sheared protein to ensure the bioactivity of Collagen.

本品原料来自纯净、无污染的特定海域中的深海鱼类，采用具有自主知识产权的限制性酶切制备多肽技术（Limited Enzymic Degraded Technology），在低温下使用复合生物酶制剂，通过特殊低温萃取工艺提取有效成分，通过特异性的剪切蛋白质，保证了胶原蛋白原有的生物活性，并将原分子量为30万左右的胶原蛋白分子降解成3500左右的胶原蛋白多肽（约含30个左右的氨基酸残基），其分子长度降至30—50nm，集中度达到99.5%以上，呈白色或淡黄色，无异味，无杂物，不添加任何激素和化学物质，没有任何毒副作用。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。