生物技术的

In this paper,the progress,especially combining gene engineering of mutational biosynthesis is reviewed.

本文综述了突变生物合成技术在微生物药物领域的应用，特别是结合基因技术进行突变生物合成研究的新进展。

Nowadays, water resource shortage and water. pollution facing the whole world have promoted all kinds of technologies study in water pollution control and water reuse. With the economic development, the life standard is improving and the habited circumstance is accelerating in our country.

中文题名一体化复合式膜生物反应器应用于小区污水回用中试研究副题名外文题名 Pilot experimental study on the integrated hybrid membrane bioreactor for domestic wastewater reuse in district 论文作者迟军导师王宝贞学科专业环境工程研究领域\研究方向学位级别博士学位授予单位哈尔滨工业大学学位授予日期2003 论文页码总数148页关键词中空纤维膜膜生物反应器污水处理馆藏号BSLW /2003 /X703 /46 世界范围的水环境污染和水资源短缺，使人类积极地寻求各种水污染控制技术及水回用技术。

Comparing with the traditional personal authentication methods which always utilize codes or IC card, biometrics cannot be lost, forgotten, impost and copy, and therefore is more safe, reliable and efficient.

生物特征识别技术是利用人体固有生物特征来进行身份识别的技术，相对于传统的基于密码和IC卡的身份识别技术，具有更高的安全性、可靠性和有效性。

This technology has theadvantages of simplicity, accuracy, inexpensiveness, sensitivity and well-selectivity,and also can be compatible with DNA chips technology.

DNA电化学传感技术是一门涉及生物化学、电化学、医学及电子学等领域的交叉学科，具有简单、可靠、价廉、灵敏和选择性好等优点，并且与DNA生物芯片技术兼容。

Biosensor technique is a new microanalysis method which is widely used in the field of biology, medical sciences and food.

摘 要 生物传感器技术是一种全新的微量分析技术，目前已经广泛应用于生物、医学及食品等领域。

The scope includes: Bioluminescence, chronobiology, DNA repair, environmental photobiology, photocarcinogenesis, photochemistry of biomolecules, photomedicine, photomorphogenesis, photomovement, photoreception, photosensitization, photosynthesis, phototechnology, spectroscopy of biological systems, UV and visible radiation effects and vision.

该杂志出版的范围涵盖：生物发光、时间生物学、DNA修复、环境光生物学、光致癌过程、生物分子光化学、光医学、光形态发生、光运动、感光、光敏作用、光合作用、光学技术、生物系统光谱学、紫外线和可见光效应及其显示等。

Face recognition is an active subject in the area of biometrical recognition technology,and lots of achievements have been obtained.

生物特征识别技术在身份认证中发展十分迅速，得到了人们的广泛关注，而人脸识别是生物特征识别技术中一个活跃的研究领域。

The metobolic mechanism of MLF and MAF,the effects of some external factors on MLF and MAF,and utilization of modern fermentation technology(immobilized cell and enzyme,membrane bioreactor)in bio－deacidification were reviewed and discussed in this paper.

本文对MLF和MAF生化反应机理、影响MLF和MAF的环境因素以及现代发酵工程技术(固定化细胞、酶技术、膜生物反应器)在葡萄酒生物降酸工艺中的应用也进行了回顾。

These indicates bioactive peptides in Chinese forest frog skins have great potential for the exploitation and utilization. S_(180) cells were inoculated hypodermically at the concentration of 1×10~6 cells per mouse and the bioactive peptides in Chinese forest frog skins were resolved into aseptically distilled water to make injection liquid. After injected continuously 15 days, the inhibition rate of tumor was calculated. The induction of tumor cell apoptosis of active peptides in Chinese forest frog skins could be detected by morphological

将S_(180)胃肉瘤细胞接种至小鼠皮下，选取中国林蛙皮肤生物活性肽不同浓度样品液，连续注射种瘤小鼠15天后，计算抑瘤率；采用透射电镜观察细胞形态学变化、DNA梯度琼脂糖凝胶电泳技术、MTT比色法、流式细胞检测等技术观察中国林蛙皮肤生物活性肽对K562肿瘤细胞凋亡过程的诱导。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。