生物差异

Therefore, a experimentent with three exposure types (early cleavage exposure, 0hpf; gastrula exposure, 8hpf and 0hpf exposure but stop after 8 hours, 8h stop) were designed to investigate the toxicity and bioaccumulation of bisphenol A in zebrafish embryo in different stages.

为研究胚胎在不同发育阶段对外源化合物的毒性敏感差异，以0hpf、8hpf和8h终止3种暴露方式进行染毒，探讨斑马鱼胚胎在不同暴露时段对双酚A毒性和生物蓄积特征。

Group B, based on the group A, single-door laminoplasty with reconstruction of posterior cervical ligamentous complex was performed.

目的：比较重建颈后方韧带复合体单、双开门椎板成形术后生物力学的差异。

The optimal reaction parameters of methanolysis of soybean oil by lipase-catalysis weredetermined as follows: 15wt% of lipase, 55℃of temperature, petroleum ether as solution,methanol was added by 6 times.

对酶法催化豆油和地沟油的对比没有显著差异，说明脂肪酶是催化游离脂肪酸含量高的油脂生产生物柴油的有效催化剂。

Monocellular Raman spectrums of normal liver cell line (LO2) and carcinoma liver cell line (SMMC-7721) were studied with laser tweezers Raman spectrums system and each cell was investigated in three different points, the result of which showed; significant differences of average Raman spectrums were found between the normal cell and the carcinoma one; And, the spectra line of carcinoma cell became weak at the whole; Additionally, the spectral peak intensity ratio of normal cell at 1658cm^(-1) and 1450cm^(-1) was 0.63 while that of carcinoma was 0.99; Moreover, the structure and the amount of protein; nucleic acid, lipids and other such molecules had also changed.

本工作利用激光镊子拉曼光谱系统研究了人正常肝细胞株(LO2)和肝癌细胞株(SMMC-7721)的单细胞拉曼光谱，对于每个细胞在不同部位测三个点。实验结果显示：正常细胞和癌细胞的平均拉曼光谱存在显著差异；癌细胞谱线强度整体变弱；正常细胞的1658cm^(-1)处峰和1450cm^(-1)处峰的强度比值为0.63，癌细胞的为0.99；癌细胞的核酸、蛋白质、脂类等重要生物分子在结构或含量上都发生了不同的改变。

The mechanical properties of the patellar tendon were significantly correlated to its mass density.

结果 髌腱的生物力学性质基于性别上的差异均无显着性，而与其密度呈正相关。

The mechanical properties of the patellar tendon were significantly correlated to its mass density.

结果 髌腱的生物力学性质基于性别上的差异均无显著性，而与其密度呈正相关。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

It was found that accession C108 had the highest As accumulation in shoot, being significantly higher than all other accessions. There was a significant difference among the accessions of Pteris vittata in both transporting factor and biological factor.

kg^(-1)基因型C108地上部积累砷最多，显著大于其它蜈蚣草基因型；研究还发现，蜈蚣草基因型间的转运系数和生物富集系数也存在显著差异。

By gene microarray analysis,we also identified CAPG,S100A14, LGALS1,and IEX-1 were overexpressed in the highly invasive subcell lines.

我们针对上面38个在两张侵袭芯片中共同上调表达的差异基因，通过表达谱分析和生物信息学分析选取了4个基因作预示胃癌侵袭转移的候选标志物。

To this end,we used the Transwell system to select two highly invasive subcell lines SNU5-P3 and PAMC82-P3 from minimally invasive parent cells,and compared gene expression in paired cell lines wit...h high and low invasive potentials.38 genes were commonly upregulated in the two highly invasive subcell lines.

通过全基因组芯片比较SNU5-P3和PAMC82-P3与其亲本细胞的基因表达谱，获得了38个在两张芯片共同上调表达的差异基因，其中16/38(42%)已报道是与侵袭转移功能相关的基因。LOXL2是一个在两张芯片共同上调表达的基因，通过生物信息学分析和细胞表达谱分析，表明LOXL2可能是胃癌侵袭转移密切相关的功能基因。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。