生物学上

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

The methods based on dualism are: the argument of analogy, hypothesis-deduce, cloud chamber imprint analogy, etc; while the methods based on monism are: logical behaviorism,being of Strawson, methological behaviorism, physicalism, etc. Since the 20 century, concerning the other minds problem, certain important achievements and empirical material were offered along with the great development made by modern scientific technology (esp. the Neurobiology, cognitive neuroscience,and the measuring and testing techniques of brain), the knowledge combination and intersecting cooperation research done by philosophers and biological scientists.

对于他心问题的解决方案，建立在二元论基础上的方法有：类比法，假说—演绎证明与"云室"痕迹类比等；建立在一元论基础上的方法有：分析行为主义，斯特劳森的人论，心理学行为主义，物理主义等。20世纪以来，随着现当代科学技术（尤其是神经生物学、认知神经科学和大脑测试技术等）的深入发展，哲学家与生命科学家的知识联姻和交叉合作研究，提供了关于他心问题的若干重要成果和实证资料。

Cucumber mosaic virus isolation CP310 was isolated from Capsicum annuum.By detecting 14 kinds of host plants,Nicotiana glutiana and Nicotiana clevelandii with CP310 showed dead symptoms and Lycopersicon esculentum and Nicotiana tobacum with CP310 showed dwarfish symptoms.

在辣椒上分离到一株黄瓜花叶病毒分离物CP310，对其进行14种寄主植物生物学反应测定，发现CP310在心叶烟、克里夫兰烟等植物上表现致死症状，在番茄、烟草表现为植株明显矮化。

Objective To establish a method of culturing human amniotic epithelial cells in vitro and to study its biologic features.

目的建立体外培养人羊膜上皮细胞的方法，观察体外培养的羊膜上皮细胞的生物学特性。

And gene engineering used in the store and innovation of germ plasm.

指定学生课外自学内容，适当介绍生物化学、分子生物学等最新技术在种子检验及品质分析上的应用，组织培养、基因工程技术在种质保存及创造上的最新进展，扩大课堂教学的信息量。

Because the molecular mechanism of parturition is still unknown, there is no perfect method to detect, prevent and therapy preterm delivery.

由于分娩的确切机制在分子生物学水平上还不十分明了，临床上至今对孕妇早产没有理想的的检测、预防和治疗方法。

The content and structure ofthis course were completely modified based on biology, sitology and pharmaceutics.

本课程是在《生物学》、《营养学》、《药用学》的基础上，进行全面调整和结构体系上的修改。

The objective of this study is to picture the signal tranduction pathway of apoptosis induced by MC so as to further investigate the impact of MC on transcription, expression and regulation of apoptosis gene and the related biological effects; illustrate the toxicological mechanism of MC; as well as discover the new biomarkers invovled.

本研究工作的目的是通过功能基因组分析的技术平台，探讨微囊藻毒素诱导肝组织细胞凋亡的信号转导通路，以便在此基础上，进一步深入研究该毒素对凋亡功能基因在转录、表达、调控水平上的影响，及由此引发的生物学效应；揭示微囊藻毒素的凋亡毒理机制，同时发现新的生物标记物。

Researchers have revealed the complete mitochondrial genome of one of the world's most celebrated mummies, known as the Tyrolean Iceman or Otzi. The sequence represents the oldest complete DNA sequence of modern humans' mitochondria, according to the report published online on October 30th in Current Biology, a Cell Press publication.

研究人员揭示了世界上最有名的木乃伊--提洛尔冰人的全部线粒体基因组序列，该序列代表了现代人线粒体的最古老的完整DNA序列，这项研究报告的网络版发表在10月30日出版的cell系列杂志中的《当代生物学》上。

Because different alleles of S-RNase are usually coded by the different amino acids in HV region, therefore,besides study on the genomic level, other approaches on the mRNA and protein level or cross-pollinated test are also important to confirm the S genotypes.

因此，除了在分子生物学的水平上进行研究外，其他方法如mRNA、蛋白质和杂交授粉等水平上的研究在确定S基因型上也同样重要。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。