生物化学

WTBX〗Chlamydomonas microsphaera〖WTBZ〗 was dominant species in winter,and the dominance index changed from 7143% to 9628%.

同时，生物学评价显示，蠡湖冬季水质劣于春季，与化学评价结果相一致；但从评定级别看，生物评价认为，2007年冬、春季节蠡湖水质分别处于重污染和中度污染状态，较化学评价的污染程度重，显示出两种方法的差异性。

The journal publishes papers embracing all aspects of histochemistry and microtechnic in all areas of the biological sciences from botany to cell biology to medicine, including in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, image analysis, cytogenetics and automation or computerization of investigative procedures.

杂志刊载了内容涉及生物科学中从植物学到细胞生物学到医学所有方向的组织化学和显微技术所有领域的论文，包括原位杂交，细胞化学探针，放射自显影，光和电子显微镜，组织培养，图象分析，细胞遗传学和自动或计算机研究方法。

The journal publishes papers embracing all aspects of histochemistry and microtechnic in all areas of the biological sciences from botany to cell biology to medicine, including in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, image analysis, cytogenetics and automation or computerization of investigative procedures.

杂志刊载了内容涉及生物科学中从植物学到细胞生物学到医学所有方向的组织化学和显微技术所有领域的论文，包括原位杂交，细胞化学探针，放？？？？？？？自显影，光和电子显微镜，组织培养，图象分析，细胞遗传学和自动或计算机研究方法。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

Nano titanium dioxide is of favorable biological and chemical inertness, strong oxidizability, low cost and long-term stability against photocorrosion and chemical corrosion. Nano TiO_2 and TiO_2/SiO_2 thin films are used photocatalysis degradation materials and self-cleaing materials, and can kill bacteria and virus.At present it is the hot spot in the research of photocatalysis, and it is of great value in the practical applications.

由于纳米TiO_2具有良好的生物和化学惰性、较强的氧化性能、低成本、以及对光腐蚀和化学腐蚀的稳定性等，故其薄膜不仅能用作光催化降解和自洁净材料，还能杀死细菌及病毒，是目前光催化材料的研究重点，具有广阔的应用前景。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

PH, dissolved oxygen and chemical indexes for instance total nitrogens, total phosphorus, chemistry oxygen depletion; appraised phytoplankton species complexity; numerated phytoplankton cell density; analyzed various kinds of biological diversities.

主要测定了水温、透明度、pH、溶解氧等物理指标；总氮、总磷、化学耗氧量等化学指标；同时对浮游植物的种类组成、细胞密度和生物多样性指标进行了鉴定、记数和分析。

Key players at the base of the marine food web are diatoms (unicellulor algae with silicified cell walls) and their main predators, the herbiorous copepods.

本文对硅藻产生的防御性物质oxylipins的化学生态学及其在极低浓度下如何其作用进行了综述，同时对其生物合成、测试和化学防御对浮游生物群落结构的影响等进行了讨论。

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Some specific examples on the exploitation of core-shell particles in drug delivery, bioassay and biolabeling applications are given.

本文综述了化学蛋白质组学的最新进展、有关技术及其在生物医学和药物研发等方面的应用，并对化学蛋白质组学的发展趋势和前景进行了讨论。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。