生物化学

Screening toxic proteins to M alternatus from entomogenous fungi is the base of control pest. It will reveal the mechanism how the fungi infect their host from the view of biochemistry and molecular biology, and base for culture trees of anti-longhorn beetle.

本研究是从虫生真菌中寻找对松墨天牛有毒杀作用的蛋白，该研究可从生物化学和分子生物学的角度揭示虫生真菌的致病机理，同时为培育抗天牛苗木奠定基础。

These would hinder the effective envenomation administration, which is also become a bottleneck for exploitation and application of this resource.

以往关于中介蝮的形态、动物区系、食性及生态、毒液的生物化学与毒理学等方面已有研究报道。

The difference between the identification methods of the Hericium erinaceus were compared systemly in terms of traditionary biology, biochemical and molecule biology in this article.

本论文从传统的生物学、生物化学和分子生物学三个方面，对猴头菌种的鉴定方法进行了比较系统的研究。

The purpose of this experiment was to build a suit of molecular biological and biochemical fingerprints methods about Radix Gentiana to meet quality standards of international.

实验的目的是要根据国际上关于中草药的质量检测标准建立一套中药龙胆分子生物学及生物化学的指纹图谱检测技术。

AIM: From the views of biochemistry, histology and molecular biology, this study observed the effects of 4-6 Hz vibration on concentration of hexosamine, the expression of mucin 5AC mRNA in gastric mucosa and histomorphology, and investigated the effects of body vibration on rats' gastric mucus secretion and mucosal structure.

邓 艳，陈伟华，宋小丹，张源淑南京农业大学动物医学院，农业部生理生化重点开放实验室，江苏省南京市 210095目的：从生物化学、组织学和分子生物学角度观察4~6 Hz振动对大鼠胃黏膜氨基己糖含量、黏蛋白5AC mRNA表达及其组织形态学变化，探讨振动对大鼠胃黏膜黏液分泌及形态的影响。

1Chen kangli, 2yan meijuan, 1zhang ying （1department of biochemistry and molecular biology, shanghai medical college, fudan university, shanghai, 200032; 2jiangsu key laboratory of neuroregeneration, nantong university

作者单位：1复旦大学上海医学院生物化学与分子生物学系，上海 200032； 2南通大学江苏省神经再生重点实验室

2Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University, Seoul 130701

1吉林大学第一医院神经外科，长春 130021；2庆熙大学医科大学生物化学与分子生物学教研室，首尔 130701

The major direction of the research within the first year will be outlined as following:( 1 )The background study, collection, analysis, and identification of the possible metabolites such as protease, hemolysin, hemagglutinin, cytotoxin, leukocidin, and lipopolysaccharide;( 2 )The collection of the pathogenic factor such as outer membrane protein of vibrio spp.;( 3 )The analysis of pathogenic factor through the application of SDS-PAGE technique; and ( 4 )The activity assay of outer membrane protein on substrate to characterize the possible function or study the reaction mechanism.

致病因子之收集：将利用生物化学之方法，以弧菌属细菌外膜蛋白质为目标，收集与溶血素相关之致病因子。3。致病因子之分析：将利用SDS-PAGE电泳，对所收集得到之致病因子进行分析，并估算其可能之分子量。4。致病因子之活性测试：将利用所纯化之外膜蛋白质进行对受质活性之测试，以确定其功能或可能之作用机制。

The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明：成功构建了pET22b-lysB表达载体，并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白；His-LysB具有分解脂肪的能力，属于脂肪酶；生物化学特性分析表明：丁酸对硝基苯为水解底物，His-Lys热稳定性不佳，30℃以下比较稳定，随着温度的升高，稳定性逐渐降低；该蛋白具有较高的pH值适应性，pH 5.0~9.5范围内稳定性较高；在23℃和pH 7.5时酶活力最高，其比酶活为1.3 U/mg；金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

Its long-term vision for research is focused on three areas, namely nutrition/nutritional biochemistry, monogastric production systems and ruminant production systems.

该系长期的研究目标集中于三个方面，即营养学与营养生物化学，单胃动物生产系统，和反刍动物生产系统。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。