生根的

The invention provides a method for producing transgene sugarcane quickly with pmi genes. The method primarily comprises the steps of establishing a mannitose screening system; transformation of a gene gun; acquisition of resistant callus; differentiation and screening of the resistant callus; acquisition and rootage of resistant plantlets; molecule detection of resistant plantlets regenerated and detection of pmi gene expression by the chlorophenol red detection method.

一种利用pmi基因快速获得转基因甘蔗的方法，主要内容包括：甘露糖筛选体系的建立，基因枪转化和抗性愈伤组织的获得，抗性愈伤组织的分化筛选，抗性苗的获得和生根，抗性再生苗的分子检测和氯酚红法检测法检测pmi基因表达。

What's worse, they think that the temperature condition is not necessary for seeds to come into leaf and that plants can also grow well without fertilization.

学生对於植物生长的另有概念包括将根与茎的名词定义错误，认为植物是「先长茎再长根」或「先长果实，后开花」；并对植物器官的功能不熟悉；缺乏植物的「花」、「果实」具有传宗接代等繁殖概念；「温度」条件对种子发芽而言是非必要的；植物有「施肥」就长得更好，没有施肥也不会怎样；知道植物可以用种子繁殖，除了种子以外可以繁殖的器官学生多数只看过落地生根用叶子繁殖，缺乏更多的实际经验。

The best optimum conditions of the Agrobacterium mediated genetic transfornation were that leaves explants were immersed in the diluter 10 times Agrobacterium bacilli at OD600 of 0.6 for 8 minutes after being pre-cultured for 2 days,and then co-cultivated for 3 days.Then these explants were transferred to selected and rooting medium which contained 50 mg/L Kanamycin and 500 mg/L Carbenicillin.According to the transformation system, 54 transgenic plants were initially screened,then after PCR analysis and RT-PCR analysis 25 transgenic plants were obtained.

根癌农杆菌介导的芪合酶基因遗传转化的最佳条件为：将预培养2 d的烟草外植体，用稀释10倍的GV3101菌株菌液（OD600=0.6）浸染8 min后，共培养3 d，然后转入含卡那霉素50 mg/L和羧苄青霉素500 mg/L的筛选分化培养基上诱导分化，待抗性芽长到2～3 cm后，转入含Km 50 mg/L和Cb 500 mg/L的筛选生根培养基上进行筛选，在以上最佳遗传转化条件下，初步筛选到54株转化体，经PCR和RT-PCR检测获得25株转化烟草植株。

At this point,"Shina" and discrimination associated with it, and take root in Japanese. 1 **** 2 years, Arthur Fu missionaries have written to him on the Chinese people's perception, brought together into a 300-page "Chinese characteristics", the book lists China's 26 features, Including the "no concept of time","no accurate idea","nature of the beat around the bush" and "intellectual confusion" and "foreign lightly," and "observe the same old rut," and "mutual suspicion" and so on; and go: China as the Chinese community Scenery, good-looking From a distance, the stench is almost offensive.

至此，"支那"便与歧视联系在一起，并在日语中生根。1****2年，传教士明恩傅把他陆续写成的关于中国人的观感，汇集成一本300多页的《中国人的特性》，书中列举了中国人的26个特点，其中包括"没时间观念"、"没有准确观念"、"拐弯抹角的天性"、"理智混乱"、"轻视外国"、"因循守"、"互相怀疑"等；并狂言：中国社会如同中国的景致一样，远看好看，近则臭气难闻。

Daria will celebrate with friends and colleagues from the past and present of her career including Daniil Simkin and Hermann Cornejo from ABT, Stuttgart Ballet's Friedemann Vogel and current partner Vadim Muntagirov.

里娅将庆祝的朋友和同事从过去和现在的她的职业生涯包括丹尼尔西姆金和赫尔曼科尔内霍的ABT生根粉，斯图加特芭蕾舞团的弗里德曼Vogel和现在的合作夥伴瓦迪姆Muntagirov。

Whether it is in precipitous cliffs or Hegou Tin Road, I do not care about the survival of the environment, as long as there is soil, there is sunshine, there is the survival of the gap, I will come out strong and long, as long as there is a small humidity, temperature and oxygen, I will take root and grow sturdy body to add green to the earth.

无论是在山尖崖峭，还是在河沟田道，我从不在乎生存的环境，只要有泥土，有阳光，有生存的缝隙，我都会顽强的长出来，只要有一丁点湿度、温度和氧气，我都会生根发芽，长出壮实的身子来，为大地增添绿色。

The declining of the value of IAA/ZR was beneficial to callusing, the other way around was beneficial to rooting. The higher value of IAA/ABA was better for root-promoting during the shape of rooting anlage and the emergence of root.

IAA/ZR比值的下降有利于愈伤组织的形成，上升有利于不定根的形成；在根原基形成和不定根出现期，高的IAA/ABA对生根有利。

At first, as those charitable actions were for helping to mission, missionary charity had strong religious character and colonialism. With the deep development of Christianity in the Republic of China, the religious character of missionary charity desalinated slowly and became worldly.

最初的教会慈善机构一般都为便利传教而设，因此带有浓厚的宗教与殖民色彩；进入民国以后，随着基督教在华的生根，教会慈善事业的宗教色彩逐渐淡化而趋向世俗化。

This is the plant kingdom, there are nearly 800 kinds of rare plant take root here, thrive here from Russia, Kazakhstan natural extension to our country the only one in Southern Siberia Taijialin Nature Reserve, at an altitude of 1,300 meters to 2,300 meters on the slopes of a lush natural growth of larch, spruce, fir, poplar, birch and precious Xinjiang Pinus.

这里是植物的王国，有近800种珍惜植物在这里生根发芽、茁壮成长，这里是从俄罗斯、哈萨克斯坦自然延伸至我国境内的唯一一片南西伯利亚泰加林自然保护区，在海拔1300米至2300米的山坡上自然生长着茂密的落叶松、云杉、冷杉、白杨、白桦以及珍贵的新疆五针松。

New plantlets, borne on the end of long strap-shaped leaves, take root easily, hence "walking" iris.

长在长块状的叶端的新出的小植物，很易就能生根，由是英文名 'walking'会走路的

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。