生根

Differentiation rate, Callus induction rate and and Root Induction rate individual was up to 100%.

确定了筛选欧美杨108转化体及选择生根培养时的抗生素浓度。

IBA and IAA could promote each other when they were used together in medium QL.

IBA与IAA在QL培养基中配合使用对诱导生根有增效作用。

Nigra during the experiment. Successfully established the transformation procedure Agrobacterium-mediated of Populus simonii×P nigra: cut vigorous leaves to 2-3 patches through the main vein after growing about 20 days, pre-culture time was 3~4days;centrifuge Agrobacterium when being developped to the logarithm growth timeOD_(600=0.5, then dilute Agrobacterium to the concentration of OD_(600)=0.3-0.4 with MS liquid culture medium or asepsis distilled water for infection;infection time was 6-15 minute;after being co-cultured 3 days, inoculate on the selecting medium to be filtrated in succession during inducing unsureness buds and rooting with Kanamycin(containing Kanamycin 40mg/L and Cefazolin Sodium 700mg/L).

成功摸索出农杆菌介导的小黑杨遗传转化体系：选取生长20d左右的健壮的小黑杨组培苗叶片，通过主叶脉剪2～3下，预培养3～4d；将培养至对数生长期的农杆菌OD_(600=0.5离心后用液体MS培养基或无菌蒸馏水稀释成OD_(600)=0.3～0.4浓度，进行浸染；侵染时间为6～15min；共培养3d后，转入到筛选培养基中进行诱导不定芽及生根阶段的卡那霉素连续筛选(含有40mg/L卡那霉素和700mg/L的头孢唑林钠)。

The amount of total corn root decreased with the increase of average soil BD.

玉米总根量和气生根数量随着整个土层平均容重的增加而降低。

: Colossians 2:7 Rooted and built up in him, and

和合本：西 2：7 在他里面生根建造，信心坚固，正如你们所领的教训，感谢的心也更增长了。

Nevski in vitro and selected several favorable media.Germ-free plants were produced with germination on MS medium. Callus was induced with B5+1.5 mg.L-1 2,4-D+0.5 mg.L-1 NAA,with leaf sheath and hypocotyls as explants.

Nevski种子消毒并在MS培养基上萌发获得无菌苗，以幼苗的叶鞘和胚轴为外植体诱导愈伤组织，经继代后进一步诱导不定芽及生根。

Dreamed of sending millions of Italian settlers to Italian East Africa and there were high hopes of turning the area into an economic asset.

而墨索里尼欲把数以百万计的移民者送到东非的殖民地中落地生根，以取得在东非的丰厚经济利润。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。