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The changes of endogenous hormones in adventitious root formation in softwood cuttings of autotetraploid grape Muscat Hamburg were studied.

以葡萄同源四倍体玫瑰香和其二倍体玫瑰香母株的嫩枝插条为试材,研究了扦插生根过程中(0~18d)生根部位皮层的内源激素含量变化。

The results showed that NAA at low concentrations 0.1~0.2 mgL^(-1 was more beneficial for root induction, the rate of rooting was up to 100%. 1/4MS was more suitable to deal with the plantlet rooting and form small bulb. The optimal ratio of IBA and AC for rooting was 2.0 mgL^(-1) IBA+0.1%AC. The in vitro growth of plantlets were the strongest, and survival rate was 100% after incubating at 4℃ for 4 weeks when the diameter of bulblet was ≥9 mm.

结果显示;低浓度的NAA0.1~02mgL^(-1有利于根的诱导;生根率均达100%;大量元素1/4MS处理更适合试管苗的生根和小鳞茎形成;"IBA+AC"处理最佳组合是2.0mgL^(-1) IBA+0.1%AC;鳞茎直径≥9mm的试管苗去顶4℃低温冷藏4周;移栽后喷施1/2MS营养液;长势最好;成活率达到100%。

The most suitable system of rooting and transplanting was established: 1/4MS+0.1~0.2 mgL^(-1) NAA or 1/4MS+2.0mgL^(-1) IBA+0.1% AC were the best formula, and the best transplanting for in vitro plantlets was to choose diameter of bulblet that was about ≥ 9mm and cut its leaves, after 4℃ low-temperature refrigeration for 4 weeks, and then spray with 1/2MS or MS nourishing cream.

本试验获得适宜亚洲百合的生根移栽技术体系:生根最佳配方为1/4MS+0.1~0.2mgL^(-1) NAA或1/4MS+2.0mgL^(-1) IBA+0.1%AC;最佳移栽技术为选取鳞茎直径57mm的试管苗去顶;4℃低温冷藏4周后移栽;栽后喷施1/2MS或MS营养液。

When scales are proposed by nutrition solution, the information of bulblet is very regular: information of bulblet is almost located in inside 1—2mm of wound, the root is formed below of bulblet.

营养液浸种鳞片,小子球的分化很有规律性,据试验观察,小子球发生大都在伤口腹内侧1—2mm处,在沿切口呈线状排列,腹外侧和愈伤组织处只占1%左右,小子球形成后多在其下部生根;而一般基质无菌水培养的多先在鳞片下部生根,后形成小子球,即较前者推迟一周。

Dynamic changes of three protective enzymes activity and MDA content of Catalpa 'Jinsi' and C.'Yuanjichangguo' during softwood cutting time were studied. And the relations between its dynamic changes and rooting rate were analyzed and discussed.

以金丝楸和圆基长果楸的嫩枝为材料,研究了ABT处理下扦插生根过程中3种保护酶活性和丙二醛含量的动态变化,分析了其变化规律与生根的关系。

With the corm and scale of A. albus in Zigui County in Hubei Province as tested material, MS was used as the basic medium with different hormones to respectively match 10 kinds of media for induction, differentiation for tissue culture of A. albus The induced clumpy buds were cut into single plants and were put into the rooting medium of MS+NAA 0.1-0.5 mg/L and the effects of different hormone matching on callus formation, bud differentiation and rooting were studied.

方法]以湖北省秭归县的花魔芋球茎和鳞片作为外植体,以MS培养基为基本培养基,添加不同激素,分别组配成10种诱导培养基和分化培养基进行组织培养,然后将诱导的魔芋丛芽切成单株后接入MS+NAA0.1~0.5 mg/L生根培养基,研究不同激素配比对愈伤组织形成和芽分化以及生根的影响。

The results showed that the rooting rate reached 83.2%~96.3% in 1/2 MS medium with IBA1.0~1.5 mg/L, The rooting rate of stem with top was significantly higher than that of stem without top; Snicking in stem base increased root number obviously, The appropriate substratum for transplanting was perlite and soil (1:1), The growth tendency of transplanting plantlets in May was slow-fast-slow.

结果表明:采用1/2MS培养基添加IBA1.0~1.5 mg/L,生根效果最好,平均生根率达83.2%~96.3%;带茎尖茎段的生根率显著高于不带茎尖茎段;茎段基部刻伤处理后较对照生根量明显增加;酸枣带根组培苗适宜的移栽基质为1:1的珍珠岩和土;5月份移栽下地的组培苗,其生长呈现慢-快-慢的趋势。

The root inducation rate is 100% on the cotton, pearl rock and agar medium, but the number of root induced is maximum. The average number is 5.47. The young root possess many multistage branchs. The transplant effect also showed pearl rock is ideal substitute for agar.

珍珠岩、琼脂和脱脂棉的生根率都达到100%,生根数以珍珠岩最高,平均5.47条,幼根具多级分枝,试管苗移栽也表明,珍珠岩在地灵试管生根中是琼脂的理想替代物。

Based on taking the orthogonal design experiments and by using the technique of plant tissue culture, the experiment-system of tissue culture and rapid propagation of Mozzie buster was successfully established.

运用正交设计方法,利用植物组织、细胞培养技术,成功地建立了驱蚊草组织培养快繁技术体系。研究出:不定芽诱导的最适培养基为MS+6-BA0.2mg/L+NAA0.3mg/L,增殖倍数为6.73;生根培养基为1/2MS+NAA0.4mg/L,平均每株生根数为11.2条,生根率为90.0%。

Use IBA 1000mg/L retting cuttings 30s can get high rooting rate. Culture outside tube is a fit means to induct root.

用IBA1000mg/L作生根促进剂处理30s瓶外生根生根率最高,达到58.33%。

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Fancy gold-plated dangling earrings with facetted White Opal crystals.

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In measurements of a day,generallyspeaking,the photosynthesis of birch in mesophytic habitat is better than that in xerophytichabitat(peak values are 12.8,10.33μmolCO2m-2s-1 respectively);that of sexual birch inmesophytic habitat is better than that of clone birch(peak values are 9.87,6.71μmolCO2m-2s-1respectively);that of young tree is better than that of seedling(peak values are12.37,10.05μmolCO2m-2s-1 respectively).

在一天中的各个时刻,总体说来,中生生境生长的白桦光合作用超过旱生生境生长的白桦光合作用(净光合速率峰值分别为12.8、10.33μmolCO2m-2s-1);白桦幼树的光合作用超过白桦幼苗(净光合速率峰值分别为12.37、10.05μmolCO2m-2s-1);中生生境有性白桦的光合作用超过无性白桦的光合作用(净光合速率峰值分别为9.87μmolCO2m-2s-1、6.71μmolCO2m-2s-1)。