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Saponin composition of common sugar glucose, galactose, rhamnose, arabinose, xylose and glucuronic acid, galacturonic acid, etc., are often associated with C3 Sapogenins-OH-bit connection into effect.

组成皂的糖常见的有葡萄糖、半乳糖、鼠李糖、阿拉伯糖、木糖及葡萄糖醛酸、半乳糖醛酸等,常与皂元C3位的-OH连接成

The results show that the duration of the 3 phases during the culture of cell suspension is related to the inoculum size, the initial nutrient concentration, the feeding of nutrients and the culture conditions such as temperature and dissolved oxygen, that the maximum cell yield reaches about 12g/L for batch culture or 35g/L for fed-batch culture in dry weight, that the saponin content of cells quickly increases in the bloom phase and keeps on increasing until the early stage of the declining phase, with a maximum saponin content of about 60~70g/kg in dry weight, that the conductance value of cell suspension decreases from more than 8000μS to less than 600μS with the growth of cells, but increases when the cells autolyze, and that part of cells turns red in the later culture, which has no apparent effect on the saponin content.

结果显示:细胞3个生长时期的持续时间与接种量、初始营养浓度、营养流加情况及培养条件有关;分批培养和流加培养时的最大细胞得率分别为12g/L和35g/L;细胞的皂含量在旺盛生长期快速上升,并保持增长至衰退期前期;干细胞中最大的皂含量达60~70g/kg;随着细胞的生长,培养液的电导值从8000μS以上下降至600μS以下,细胞自溶后逐渐回升。另外,培养后期部分细胞颜色变红,但没有发现颜色转变对皂含量造成影响。

In Chapter 1, eleven glycosides (six oleanolic acid saponins, five hederagenin glycosides) were isolated from Decaisnea fargesii Franch.

第一章从木通科猫儿屎植物中分离,鉴定了十一个三萜糖化合物,其中六个为齐墩果酸,五个为常春藤

The infrared test indicates: all the saponins absorbing waves are similar, they contain functional groups such as aliphatic hydrocarbons, primary aliphatic alcohols and secondary aliphatic alcohols(Aliphatic carboxylic acids in sample 1 and 2) estimated by basic analyzed chart. They may contain long carbon chain with hydroxy, isomaltose and galactose estimated by particular analyzed chart.

红外吸收表明:皂组分的波形都非常相近,经过基础图谱分析可以发现皂中含有脂肪烃、脂肪族伯醇、脂肪族仲醇等官能团,样品1与2还含有脂肪酸官能团,经详细图谱检索,说明皂含有带羟基的长碳链、异麦芽糖和β-D-半乳糖。

This paper provides a comprehensive survey of the chemical structures of all glucosinolates which have been identified up to now, summarizes the processes of their conversion to isothiocyanates and other products by myrosinase,and discusses the various factors which affect their degradation to special products.

介绍了迄今已发现的硫的化学结构,以及通过芥子酶的作用,硫降解成异硫酸氰酯和其他产物的途径,讨论了影响硫降解的因素。

With this set of criteria, the literatures published 3—O—L—rhamnosyl—(1→2/1→4)—L— rhamnoside, 3—O -D—xylosyl—(1→2/1→4)-L-rhamnoside and 3-O-D — glucosyl-(1 →2/1→4)—L— rhamnoside structures of anhydroicaritin and desmethylanhydroicaritin were re-evaluated.

本文以上述双糖1→2连接的光谱标准,对文献报道的脱水淫羊藿素和去甲脱水淫羊藿素3—O—L一鼠李糖—(1→2或1→4)—L—鼠李糖、3—O—D—木糖—(1→2或1→4)—L—鼠李糖及3—O—D—葡萄糖—(1→2或1→4)—L—鼠李糖类化合物的结构进行了重新评价。

The survival rates of CEM cells cultured with cis-diamminedichicloroplatinum added 24 h later were higher than that cultured with hTERT ASODN and DDP added 24 h later. The survival rates of CEM cells cultured with DDP were similar with that cultured with hTERT SOND and DDP. In morphological observation of apoptotic cells using Giemsa staining, cells treated with DDP or DDP combined with hTERT ASODN ro SODN at 48 h, displayed classic apoptotic changes. Apoptosis rates of CEM cells treated with DDP for 48 h after 24 h of exposure to ASODN significantly increased. There was significant difference in the percentage of apoptotic cells of CEM cells between hTERT ASODN plus DDP and SODN plus DDP or DDP alone, respectively.

结果: hTERT ASODN作用于CEM细胞24 h再加入柔红霉素、长春新碱、足叶乙,对细胞生长的抑制分别与单用柔红霉素、长春新碱、足叶乙及hTERT正义寡核苷酸联合柔红霉素、长春新碱、足叶乙组相比,统计学上无显著差异(P>0.05)。hTERT反义核酸作用于CEM细胞24 h加入顺铂,再共同作用48 h,CEM活细胞均数为2.318×108 cells/L,与单用顺铂组(3.250×108 cells/L)及hTERT正义核酸联用顺铂组(3.175×108 cells/L)相比,对细胞抑制明显增强(P<0.05)。hTERT ASODN作用于CEM细胞24 h再加入顺铂作用48 h,细胞出现典型的凋亡形态学改变。hTERT ASODN与2.5 μmol/L顺铂联合作用于CEM细胞48 h的凋亡细胞百分率(19.47%)分别同SODN与顺铂联合作用组(6.97%)、单用顺铂作用组(6.02%)进行比较有显著差异(P<0.01)。

However, the superheating effect may also result in the degradation of thermolabile compounds. Both the data of calculated activation energy and the results of experiments indicated that quercetin glycosides degraded under the high temperature caused by microwave irradiation.

首次从理论和实验两方面研究了微波加热对以槲皮素为元的黄酮的稳定性的影响,计算得到的活化能数据和实验结果均显示微波加热产生的高温可导致槲皮素糖发生分解。

Methods 25 adult female rats were divided into the control group, low dorsage group of PNS, medium dorsage group of PNS, high dorsage group of PNS and L-NNA group after the spinal cord hemisected. Corresponding dosage of PNS was fed to the rats in the three groups of PNS every day.

25只成年雌性大鼠在施行脊髓半横断术后,被分为对照组、三七总皂低剂量组、三七总皂中剂量组、三七总皂高剂量组和L-NNA组。

The mechanical contraction of circle muscularis strips, the contractile response of dispersed smooth muscle cells, the change of 〓 stimulated by sennoside with different concentration and that stimulated by Ach after incubated with sennoside were evaluated. The translocation of RhoA by sennoside were measured by laser confocal scanting microscopy too.

检测不同浓度的番泻对小肠环行肌条和游离环行平滑肌细胞的收缩作用及对〓的影响,然后测定番泻对Ach刺激的小肠环行肌条、游离环行平滑肌细胞收缩反应和细胞内〓变化的影响作用,并通过免疫荧光实验检测番泻对RhoA的活化和空间转位的影响。

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