甘露

A pectinase from CXJZ95-198 was purified. Results have made know:(1) The changes of protein content secreted by CXJZ95-198 in the five media glucose mannose xylan mannosan and pectin were 1.28mg/ml, 1.17mg/ml, 0.94mg/ml, 0.75mg/ml and 0.73mg/ml.The pectinase activity was maximum in the mannose (79.39U/ml) with the order of glucose(73.33 U/ml) mannosan(24.25 U/ml) xylan(17.45 U/ml) and pectin(15.98 U/ml).

在本研究条件下，得到如下结论：(1) CXJZ95-198菌株在以葡萄糖作为碳源的培养基中蛋白质含量变化最大，达到了1.28mg/ml，其次依次为甘露糖、木聚糖、魔芋粉和果胶培养基，分别为1.17 mg/ml，0.94 mg/ml，0.75 mg/ml，0.73 mg/ml；在这五种培养基中，以甘露糖培养基中果胶酶活性最高，达到了79.39 U/ml，其次依次为葡萄糖培养基，魔芋粉培养基，木聚糖培养基，最后为果胶培养基，分别为73.33 U/ml，24.25 U/ml，17.45 U/ml，15.98 U/ml。

This paper is mainly focus on the modification of konjac glucomannanwith mannanase and monochloroacetic acid.

本文以魔芋葡甘露聚糖为原料，利用甘露聚糖酶和氯乙酸对KGM改性。

The best combination of isolating protoplast of Mozzie buster was the enzyme mixture containing 2.0% Cellulase R-10, 0.4% Pectinase, 0.5M mannitol as osmoticpressure, 27℃under dark 80r/m rotating enzymolysis 8h, and the highest yieldachieved to 1.67×10~6per gram, the protoplast vitality was 87.4%; The bestcombination of isolating protoplast of Ocimum basilicum was the enzyme mixturecontaining 1.6% Cellulase R-10, 0.4.% Pectinase, 0.5M mannitol as osmotic pressure,27℃under dark 80r/m rotating enzymolysis 8h, and the highest yield achieved to1.67×10~6 per gram, the protoplast vitality was 87.4%.

本研究对酶解最佳条件进行研究，得出分离驱蚊草原生质体酶解条件以CPW-13进行质壁分离1h，2.0％纤维素酶和0.4％果胶酶，0.5M甘露醇为渗透压，27℃黑暗下80r／m酶解处理8h为好，产量可达1.67×10~6个／g，原生质体活力为87.4％；分离罗勒原生质体酶解条件以CPW-13进行质壁分离1h，1.6％纤维素酶和0.4％果胶酶，0.5M甘露醇为渗透压为好，27℃黑暗下80r／m酶解处理8h，产量可达2.14×10~6个／g，原生质体活力为67.8％。

The first, selecting Mannitol of polyhydric and Dextran ofpolysaccharide as subsidiary material, In accordance with differentusing quantities, making up eight recipes, freeze-drying test is madewith eight recipes differently. By adjusting recipe, to select themethod of two factors three levels. The best recipe determined is thatof Mannitol(160mg/bottle) and Dextran(40mg/bottle), Solving theproblem of description、moisture(≤1.5%)、solution(≤6s)、stability(95.0～110.0%) etc., The quality of preparation has beenimproved.

首先，选择赋形剂多元醇类的甘露醇和多糖类的右旋糖酐作为辅料，按照不同的用量，组合成8种处方进行冷冻干燥试生产，采用二因素三水平的满因子分析法，通过处方调整，确定甘露醇160mg／瓶、右旋糖酐40mg／瓶为最佳处方，解决了冻干制剂在性状、水分(≤1.5％)、溶解性(≤6s)和含量稳定性(95.0～110.0％)等方面的不足，提高了产品质量。

MethodsGuided by the contents of mannitol in Sparganium stoloniferum, uniform design, response surface analysis and path analysis were used to optimize microwave extraction process by combining with single factor experiment.

方法以甘露醇含量为响应指标，通过均匀试验设计，采用二次响应曲面和通径分析并结合单因素实验优化三棱中甘露醇的微波辅助萃取工艺。

Under the same conditions, three species were compared using fructose as substract for biotranformation to mannitol. Lactobacillus buchneri AS 1.13 has high rate of mannitol production.

在相同的条件下，比较了三种菌种以果糖为底物转化甘露醇，布氏乳杆菌AS1.13获得较高的甘露醇产率。

To determine the regulating role of mtlR in transcription of mannitol PTS operon in V.cholerae,and whether it plays a role in the ferment difference of the toxigenic and non-toxigenic strains,the mtlR deletion mutants from the mannitol rapid-ferment strain(non-toxigenic strain) and slow-ferment strain were constructed.Comparisons of the growth in M9 containing 0.2% mannitol as the sole carbon source and pH change in mannitol fermentation media of these wild strains and their mutants,indicated that mtlR is a repressor.Its repression in mtlCBA transcription was further verified with the analyses of quantitative reverse-transcriptional PCR.

通过比较快发酵菌株和慢发酵菌株mtlR缺失突变株与野生株在含0.2%甘露醇的M9培养液及甘露醇发酵液中生长、产酸等的变化，定性地证明了mtlR基因的抑制作用；另外通过定量RT-PCR进一步验证了MtlR蛋白在mtlCBA转录水平发挥负调控作用。

The 1062 amino acid sequence deduced from the 6A8 cDNA ORF is highly homologous to that of a rat ERalpha-mannosidase and that of a vacuolar alphamannosidase of Saccharomyces cerevisiae.

6A8 cDNA编码的蛋白质的氨基酸序列与大鼠的ER α-甘露糖苷酶高度同源，相同性和相似性分别高达78％和82％，与酵母α-甘露糖苷酶的相同性和相似性亦达到33％和47％。

Methods The spontaneous contraction of gastric smooth muscle strip was recorded by using physiograph in rats. The change of tension in response to different concentrations of CNP and sodium nitroprusside, co-incubated with high concentration glucose (30 mmol/L) or mannitol were detected.

取大鼠40只制备胃窦环形肌条，肌条收缩活动稳定后，对照1、2组分别加入不同浓度CNP1×10^(-8、3×10^(-8)、1×10^(-7)mol/L及硝普钠1×10^(-7、3×10^(-7)、1×10^(-6)mol/L，观察其效应，每次加药观察20 min，冲洗3次；高糖1、2组预加葡萄糖使浴槽终浓度为30 mmol/L，其后操作同对照1、2组；甘露醇组预加甘露醇30mmol/L，其后操作同对照1组；均采用多道生理信号采集处理系统计算CNP致平滑肌张力的抑制率。

Feed additives, including mannnanoligosaccharides, will reduce intestinal colonisation and shedding as salmonella with type-1 fimbria are agglutinated by the mannose component within the lumen and are excreted without adherence to enterocytes.

由于携有1型菌毛的沙门氏菌易与动物肠腔内的甘露糖分结合而脱离肠上皮细胞被排出体外，因此，选择和使用合适的饲料添加剂尤其是甘露寡糖等，将有助于降低沙门氏菌在家禽肠道内的增殖速度和减少排毒量。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。