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The present work was de-signed to study systematically the changes of anther and pollen during themannitol pretreatment period on physiology and biochemistry;and then the ef-fects of mannnitol pretreatment on androgenesis and anther culture were stu-died.The donor plants used in this experiment grow in the field.

所以本文从以上几个问题出发,以大田生长的植株作为供试材料,首次系统地研究了甘露醇预处理过程中大麦花药花粉在生理生化方面所发生的变化;以及甘露醇预处理后对大麦雄核发育和培养效果的影响。

We compare the clinical symptoms,physical sign fading time ,the Balthazar grade of CT examination after treatment for three days and by statistic methods.

目的 研究静脉使用甘露醇辅助治疗急性胰腺炎的临床疗效方法对经保守治疗好转的急性胰腺炎病例进行回顾性分析,从中随机抽取40例辅助使用甘露醇的病例和40例未使用的病例分成两组,通过对比使用组与未使用组临床症状、体征缓解的时间及治疗3天后CT检查的Balthazar评分,并进行统计学处理。

Results: compared with the control group, the amplitude and conduction velocity of biphasic action potential were gradually and significantly reduced in the hyperosmotic dextroglucose sub groups (concentrations at 20% and 40%), hyperosmotic mannitol sub group (concentration at 20%) and hyperosmotic sorbitol sub groups (concentrations at 20% and 40%) in a time dependent manner.

结果:与对照组比较,20%、40%葡萄糖组,20%甘露醇组和20%、40%山梨醇组的动作电位幅度有显著降低,传导速度有显著减慢,呈时间依赖关系;10%葡萄糖组、10%甘露醇组和10%山梨醇组高渗溶液均可使蛙坐骨神经干动作电位幅值有不同程度上升,传导速度略微减慢。

The result of biochemical test with SS2: such as glucose, ribose, mannitol, mannitose,D-glucitol, lactose, gossypose, arabinose, esculin, hippurate, starch, arginine, MR, VP.

对PCR鉴定为猪链球菌2型的菌株,进行葡萄糖、核糖、甘露醇、甘露糖、山梨醇、乳糖、棉子糖、阿拉伯糖、七叶苷、马尿酸盐、淀粉、精氨酸、MR、VP的生化实验。

A monosaccharide,C6H12O6, obtained from mannan or by the oxidation of mannitol.

甘露糖一种单糖,C6H12O6,从甘露聚糖中或将甘露醇氧化后取得

Permethylation analysis, periodate oxidation,Smith degradation, acetolysis and partial acid bydrolysis revealed the main chains of XP as mannose of (1-6)-linkages and its side chains were mannose of (1-2)linkages.

甲基化分析、过碘酸盐氧化、Smith降解、乙酰解和部分酸水解显示XP的主链是1→6连接的甘露糖,侧链是1→2连接的甘露糖。1H及13CNMR谱表明所有糖苷键均为a型,结合元素分析XP基本是酵母甘露多糖和蛋白质以及锌的络合物。

By means of GPC,IR,GC-MS,~(13)CNMR,~1HNMR,Methylation analysisetc,structural properties of PST-1 were identified as follows:The Mwof PST-1 was 3.44×10~6 Da and its optical rotation was _D~(20)=+0.110°(c0.1, H_2O); PST-1 constituted 8 simple sugars and the molar ratio was 2,4-Dimethoxy-Mannose:Rhamnose:Ara-binose:Xylose:Galactose:D-Galacturonic acid:Mannose:D-glucuronic acid=2%:5%:24%:9%:3%:1%:46%:10%;The chief bone of PST-1 was 1,3,6-linked-β-D-Man residue and the side chains contained Furanoid and Pyranoid residues.

结合GPC、旋光度测定、IR、GC-MS、~(13)CNMR、~1HNMR、高碘酸氧化法、Smith降解以及甲基化方法等分析测试方法,得到PST-1的单糖组成及结构表征,实验结果如下:红豆杉多糖PST-1是重均分子量为3.44×10~6 Da的支链多糖,旋光度为20D=+0.110~0(c0.1,H_2O);PST-1单糖组成为:2,4-Dimethoxy-Mannose:Rhamnose:Arabinose:Xylose:Galactose:D-Galacturonic acid:Mannose:D-glucuronic acid=2%:5%:24%:9%:3%:1%:46%:10%;PST-1的骨架结构为:具有1,3,6-连接的β-D-甘露糖残基骨架,侧链分枝包括非还原末端的呋喃型α-L-阿拉伯糖残基、吡喃型α-L-阿拉伯糖残基、β-D-木糖残基、β-D-甘露糖残基、2,4-二氧甲基-β-D-甘露糖残基和α-D-葡萄糖醛酸残基;侧链的糖残基也可能存在2,5-二氧-取代呋喃型α-L-阿拉伯糖基、3-氧-取代的β-D-木糖残基、6-氧-取代的α-D-半乳糖醛酸残基、6-氧-取代的α-D-半乳糖残基、4-氧-取代的α-D-葡萄糖醛酸残基和2-氧-取代的α-L-鼠李糖残基,同时后者也可能穿插在主链上。

The thesis studied the total exocellular proteins, reductant sugar and the pectinase enzyme activity of CXJZ95-198 strain in glucose, mannose, xylan, mannosan and pectin culture media.Compared enzyme activity of pectinase, xylanose and β-mannanose. The secreted proteins in ferment fluid from different culture media were analysed by SDS-PAGE electrophoresis.

本论文对CXJZ95-198菌株在葡萄糖、甘露糖、木聚糖、魔芋粉及果胶五种不同碳源培养基中胞外酶蛋白质总量、还原糖及果胶酶活性的影响进行了研究;对果胶酶、木聚糖酶及β-甘露聚糖酶在甘露糖培养基中的酶活进行了比较;对CXJZ95-198菌株在不同碳源培养基中的发酵液在SDS-PAGE电泳中分泌出的蛋白带作了比较;对CXJZ95-198菌株所产生的胞外酶系中果胶酶进行了分离纯化。

Inflorescence development was divided into four stages, the initiating expansion pseudobulb, initiating inflorescence (1-3 cm in flower stalk length), the initial-branching flower stalk, and full bloom inflorescence, respectively. The activity variations of mannan cleaving enzymes,-mannosidase (EC 3.2.1.25), endo--mannanase (EC 3.2.1.78), and the mannan synthesizing enzyme, GDP-mannose pyrophosphorylase (EC 2.7.7.13), were analyzed during different stages to better understand the role of mannan-metabolizing enzymes during inflorescence development of the Oncidium.

将花梗发育过程分为四个阶段,分别为假球茎刚膨大、刚抽梗(花梗长度约1-3公分)、花梗刚分叉以及花盛开,分析参与分解甘露聚醣之酵素-β-mannosidase(EC 3.2.1.25)及endo-β-mannanase(EC 3.2.1.78),与合成甘露聚醣之酵素-GDP-mannose pyrophosphorylase(EC 2.7.7.13),在不同阶段假球茎内的活性变化,了解在花梗发育过程中代谢甘露聚醣之相关酵素所扮演的角色。

objective to optimize microwave extraction process of mannitol from sparganium stoloniferum.methodsguided by the contents of mannitol in sparganium stoloniferum, uniform design, response surface analysis and path analysis were used to optimize microwave extraction process by combining with single factor experiment.resultsobtained response surface equation is notable at the level a.01 and the relative error of the predicted value from the model was smaller than 1%.

目的优化三棱中甘露醇的微波辅助萃取工艺。方法以甘露醇含量为响应指标,通过均匀试验设计,采用二次响应曲面和通径分析并结合单因素实验优化三棱中甘露醇的微波辅助萃取工艺。

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