甘素

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

ABSTRACT Immobilized penicillin acylase (from E.coli) was used to catalyze 7 phenylacetamido 3 E propenyl cephalosporanic acid hydrolyzation into 7 amino 3 E propenyl cephalosporanic acid. Then, trans APRA was acylated with hydroxyethyl ester of 4 hydroxy D phenylglycine to obtain trans cefprozil.

以7 苯乙酰氨基 3 E 丙烯基 3 头孢菌素 4 羧酸为原料，在青霉素酰化酶作用下，首先酶法水解得到3 E 丙烯基 3 头孢菌素 4 羧酸，过滤固相酶，滤液调pH分离得到反式APRA固体；在青霉素酰化酶作用下，反式APRA再与对羟基苯甘氨酸乙二醇酯缩合，得到反式头孢丙烯；酶法合成所得的产品与进口反式头孢丙烯对照品一致。

Assulta and the induction of GST activity by nicotine and capsicine in H. assulta provided partial supports for the hypothesis that diet breadth was governed by detoxification abilities. The results also indicated that two sibling species had different strategies of adaptation to toxic substances: H. assulta saves expenditure of nutrients and energy by restricting their countermeasures to a few potentially toxic compounds. H. armigera, by contrast, must be prepared to counter a wider range of potential toxins. 5. Compared with parental species, hybrids of reciprocal hybridization had significant difference in feeding preference for hot pepper.

结果显示，广食性的棉铃虫在中肠羧酸酯酶和谷胱甘肽-S-转移酶活性方面高于寡食性的烟青虫，但烟青虫谷胱甘肽-S-转移酶活性可以被寄主植物内的烟碱和辣椒素所诱导，表明解毒酶活性在一定程度上影响着植食性昆虫的食性广度；同时也暗示着二近缘种对有毒的次生物质采取不同的适应策略：烟青虫有选择地对付种类较少的植物次生物质以减轻营养和能量的消耗；棉铃虫则必须对付存在于其寄主植物内的种类繁多的有毒次生物质。

Ingredients: Arbutin 2%, Electrodialyzed Deep Sea Water, Mink Oil, Emu Oil, Borage Seed Oil, Calendula Officinalis Flower Oil, Lavandula Angustifolia Fiower Oil, Rosmarinus Officinalis Oil, Daucus Carota Sativa Seed Oil, Eucalyptus Globulus Leaf Oil, Pelargonium Graveolens Oil, Lecithin, Tocopherol, Hydrolyzed Collagen, Hydrolyzed Elastin, Ubiquinone Ferment, Squalene, Sodium Hyaluronate, Retinol, Glycine Soya Phytoplacenta Extract, Ceramide, Panthenol, Allantoin, Melaleuca Alternifolia Extract, Rosa Damascena Flower Extract, Propolis Extract, Centella asiatica L.

主要成分：含熊果苷2%，海洋深层水，水貂油， Emu Oil， Borage Seed Oil，金盏菊花油，薰衣草油，迷迭香精油，胡萝卜素油，蓝桉叶油，天竹葵香薰精油，卵磷脂，维生素E，水解胶原蛋白，辅酶Q10，角鲨烯，透明质酸钠，视黄醇，甘氨酸大豆提取物，神经酰胺，D-L泛醇，尿囊素，互叶白千层提取物，玫瑰果油，突厥蔷薇，蜂胶，积雪草，黄芩，甘草，金银花瓢虫，银杏叶，人参，洋甘菊，葛根等提取物

Ingredients: Electrodialyzed Deep Sea Water, Disodium Laureth sulfosuccinate, Coco-Betaine, Mink Oil, Emu Oil, Jojoba Oil, Calendula Officinalis Flower Oil, Lavandula Angustifolia Fiower Oil, Rosmarinus Officinalis Oil, Rose Hip Oil, Borage Seed Oil, Daucus Carota Sativa Seed Oil, Eucalyptus Globulus Leaf Oil, Pelargonium Graveolens Oil, Lecithin, Tocopherol, Hydrolyzed Collagen, Hydrolyzed Elastin, Ubiquinone Ferment, Squalene, Sodium Hyaluronate, Retinol, Glycine Soya Phytoplacenta Extract, Ceramide, Panthenol, Allantoin, Melaleuca Alternifolia Extract, Rosa Damascena Flower Extract, Propolis Extract, Morus alba L.

主要成分：含海洋深层水， Disodium Laureth sulfosuccinate，可可甜菜碱，水貂油， Emu Oil， Borage Seed Oil，金盏菊花油，薰衣草油，迷迭香精油，玫瑰果油，胡萝卜素油，蓝桉叶油，天竹葵香薰精油，卵磷脂，维生素E，水解胶原蛋白，弹性蛋白水解，辅酶Q10，角鲨烯，透明质酸钠，视黄醇，甘氨酸大豆提取物，神经酰胺，D-L泛醇，尿囊素，互叶白千层提取物，突厥蔷薇，蜂胶，薄荷，芝麻，何首乌，银杏叶，枸杞，荨麻腿子属，洋甘菊，葛根等提取物

We can supply monomers, such as Rutin,Baicalin,Amygdalin,Ursolic acid,limonin,?Glycyrrhizic acid, Chlorogenic acid, Paeoniflorin, Gardenoside, Paeonol, Apigenin, Naringin, Naringenin, Astragalus P.E., Soybean Isoflavones, Epimedium Extract and so on.

产品主要有单体（含量90%~98%：苦杏仁苷、柠檬苦素、熊果酸、芍药苷、栀子苷、丹皮酚、芦丁、槲皮素、黄芩苷、黄芩素、芹菜素、绿原酸、甘草酸、柚皮苷、柚皮素；标准提取物：山楂总黄酮、葛根总黄酮、黄芪多糖、枸杞多糖、大豆异黄酮等。

The results indicate the activities of gsh-px, mn-sod, cu, zn-sod, gsh, aldosterone, atrial natriuretic factor in most of rubia cordifolia l groups are higher and the content of mda and serum cortisol is lower.

结果：茜草可增加耐力运动后再力竭即刻状态下谷光甘肽过氧化物酶、锰超氧化物歧化酶、铜、锌超氧化物歧化酶、还原性谷胱甘肽活性以及醛固酮和心钠素的含量；降低丙二醛和皮质醇含量。

In order to seek for the cotton glyphosate phytotoxicity safener, matches 7 effective content processing respectively with 0.1% forchilorfenur SL and 0.004% brassins AS, the bud stage time spraying, and uses effective content 2.73 g/L glyphosate 41% SL to spraying cotton, take single application glyphosate and the blank as the comparison. The results show that the forchlorfenur and the brassins are not fast to the disintoxicating effect, but have bears the poisonous effect.

为寻找棉花田草甘膦药害安全剂，用0.1%氯吡脲SL和0.004%芸苔素内酯AS各配成7个处理，蕾期按小区喷施，再用2730mg/L的41%草甘膦异丙胺盐SL喷施棉花，以单施草甘膦和空白为对照，结果氯吡脲和芸苔素内酯对草甘膦药害无快速明显解毒效果，但有耐毒功效。

The activities of superoxide dismut ase and glutathione peroxidase were elevated with increased dur ation of the supplementation. However, no differences between the LA and HA g roups were observed.

抗氧化酵素系统中的超氧化歧化酵素(superoxide dismutas e；SOD)和麸胱甘太过氧化酵素(glutathione peroxidase；GSH-Px)的活性均随著抗氧化营养素补充时间的增长而显著上升，但高剂量组和低剂量组的上升程度相似。

To improve spinosyn-producing strain and enhance spinosyns yield, we studied the effects of glycin concentration and the operational time, temperature and lysozyme concentration on protoplast preparation of Saccharopolyspora spinosa SP06081. We also studied different regeneration media and osmotic stabilizing agents.

为了改良多杀菌素生产菌种，提高多杀菌素产量，研究了甘氨酸添加浓度、溶菌酶作用时间、温度和浓度对多杀菌素生产菌刺糖多胞菌Saccharopolyspora spinosa SP06081菌株原生质体制备和再生的影响，并考察了不同再生培养基和渗透压稳定剂对其再生的影响，确定了该菌株原生质体制备和再生的最佳条件。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。