甘油酯酶

Linolenic acid glyceride was synthesized by the catalyst of immobilized lipase.

研究固定化脂肪酶在有机溶剂中催化亚麻酸甘油酯的合成反应。

Water, Cyclomethicone, Glycerin, Sd Alcohol 40-B (Alcohol Denat.), Propylene Glycol, Melia Azadirachta Leaf Extract, Lecithin, Arctium Lappa Root Extract, Xylitol, Lactitol, Alpha-Glucan Oligosaccharide, Lactoferrin, Potassium Thiocyanate, Glucose Pentaacetate, Glucose Oxidase, Lactoperoxidase, Glycoproteins, Panax Ginseng Root Extract, Equisetum Arvense Extract, Hydrolyzed Milk Protein, Peg-8, Caprylyl Glycol, Sebacic Acid, Sodium Polyacrylate, Betula Alba Bark Extract, Cupressus Sempervirens Leaf Extract, Hamamelis Virginiana Extract, Avena Sativa Meal Extract, Humulus Lupulus Extract, Silk, Nylon-12, Polyacrylamide, Polymethyl Methacrylate, Butylene Glycol, Salicylic Acid, Lauryl Methacrylate/Glycol Dimethacrylate Copolymer, Tocopheryl Acetate, C13-14 Isoparaffin, Laureth-7, Triethanolamine, Xanthan Gum, Acrylates/C10-30 Alkyl Acrylate Crosspolymer, Phenoxyethanol, Chlorphenesin, Benzoic Acid, Methylparaben, Fragrance, Titanium Dioxide

水，环甲硅脂，甘油，Sd Alcohol 40-B，丙二醇，印度楝树叶萃取，卵燐脂，牛蒡根萃取，木糖醇，乳糖醇，α-葡低聚糖，乳铁蛋白，硫氰酸钾，葡萄糖五乙酸酯，葡萄糖氧化酶，乳过氧化物酶，糖蛋白，人参根萃取，问荆萃取，水解牛奶蛋白，聚乙二醇-8，辛乙二醇，癸二酸，聚丙烯酸酯钠，白桦萃取，丝柏叶萃取，金缕梅萃取，燕麦萃取，啤酒花萃取，丝，尼龙－12，聚丙烯醯胺，聚甲基丙烯酸甲酯，丁二醇，水杨酸，甲基丙烯酸十二酯，醋酸盐维他命E，C13-14 异烷烃，月桂醇聚醚-7，三乙醇胺，山羊胶，丙烯酸/C10-30烷基丙烯酸聚合物，苯氧乙醇，氯苯甘醚，苯甲酸，羟苯甲酯，香料，二氧化钛

We detected fasting plasma glucose by glucose oxidase, fasting insulin by chemiluminescent immunoassay, triglyceride and high density lipoprotein-cholesterol by zymology, tumor necrosis factor-αby enzyme linked immunosorbent assay, the reverse of the product of FPG and FIns is used as the insulin sensitivity index, that is, ISI=1/FPG×Fins.

血糖测定采用葡萄糖氧化酶法；胰岛素测定采用化学发光法；血脂（甘油三酯TG、高密度脂蛋白HDL-C）测定采用酶法；肿瘤坏死因子-α测定采用双抗体夹心酶联免疫法；胰岛素敏感指数采用空腹血糖与空腹胰岛素乘积的倒数，即ISI=1/FPG ×FIns。

The experiment two: enzyme preparation significantly improved average daily gainand feed conversion ratio (P＜0.05). Enzyme preparation significantly increased energymetabolizability and digestibility of crude fiber, crude protein and neutral detergent fiber,but had no remarkable effect on digestibility of dry matter, crude fat and acid detergentfiber. Enzyme preparation significantly decreased the relative viscosity of duodenal andjejunal digesta. The pH of intestine had no noticed difference in all groups. Enzymepreparation significantly decreased relative weight of gizzard, proventficulus, duodenum,jejunum and ileum. Enzyme preparation significantly increased villus size of duodenumand jejunum, and villus to crypt ratio of duodenum and ileum significantly increased too.Enzyme preparation considerably decreased ileal crypt height (P＜0.05), and didn"t affectthickness of intestinal wall. Supplementing enzyme preparation, the serum glucose, totalprotein and alanine aminotransferase, but enzyme preparation hadn"t noticed influenceupon uric acid, total cholesterol, triglyceride and high-density lipoproteins. Enzymepreparation significantly increased insulin, triiodothyronine and insulin-like growthfactor-Ⅰ. Adding enzyme preparation, the percentage of thyroid stimulating hormone andgrowth hormone in the serum increased 16.44%, 19.18% and 18.84%, 21.74%respectively, and the percentage of glucagon and thyroxine decreased 12.07%, 14.36% and 13.79%, 15.40%, but failed to reach statistical significance (P＞0.05). Enzymepreparation significantly increased (P＜0.05) the trypsin and amylase activity of duodenaland jejunal digesta, but enzyme preparation didnt affect significantly (P＞0.05) theintestinal lipase activity and pancreatic digestive enzyme. Enzyme preparation had nosignificant effect on caecal microbial population.

试验二：酶制剂显著提高平均日增重和饲料转化率(P＜0.05)；酶制剂显著提高能量代谢率及粗纤维、粗蛋白、中性洗涤纤维消化率(P＜0.05)，而对干物质、粗脂肪、酸性洗涤纤维消化率影响不显著；酶制剂显著降低十二指肠和空肠食糜相对粘度(P＜0.05)；添加酶制剂对肠道pH影响不显著；酶制剂显著降低肌胃、腺胃、十二指肠、空肠、回肠相对重(P＜0.05)，显著提高十二指肠和空肠绒毛高度，显著增加十二指肠和回肠绒毛高度／隐窝深度，降低回肠隐窝深度(P＜0.05)，对肠壁厚度影响不显著；酶制剂显著提高血清葡萄糖、总蛋白和谷丙转氨酶浓度(P＜0.05)，对尿酸、总胆固醇、甘油三酯及高密度脂蛋白浓度影响不显著，显著提高胰岛素、T_3、IGF-Ⅰ水平，添加酶制剂后，促甲状腺激素、生长激素分别提高16.44％、19.18％和18.84％、21.74％，胰高血糖素和T_4分别降低12.07％、14.36％和13.79％、15.40％，但差异不显著；酶制剂对胰腺消化酶活性影响不显著，显著增加十二指肠和空肠胰蛋白酶、淀粉酶活性，对小肠脂肪酶活性影响不显著；酶制剂对盲肠微生物菌落数影响不显著。

A method for determination of Secoisolariciresonol diglucoside, secoisolariciresinol, enterolactone and enterodiol in serum by LC/MS/MS was established. In this method,β-Glucuronidase was used to hydrolyze the serum samples, the electrospray ionization sourse and negative ion MRM scan were employed.

本实验用β葡萄糖醛酸苷酶酶解血清样品，采用电喷雾离子源，负离子MRM扫描，建立了血清中司可异罗叶松甘油二酯、司可异罗叶松酯素、肠内二醇、肠内脂的IC/MS/MS分析方法。

There are 11 gene bindings disappear in the normal group, but appear in the model group, and then disappear in the treat group, that indicates the 11 gene bindings are correlated with the epilepsy and Caohuozhimutang. After searching in the Gene Bank, 7 bindings in the 11 gene bindings are homogenic with the 8q31 ribosomal protein gene, 15p12 paraneoplastic neuronal antigen gene, 4q22 diacylglycerol kinase iota gene, xq37 FMR2 protein and 11p11 roudabout homolog 1, and 4 bindings of which are novel gene.

经NCBI美国国家基因库检索，这11条基因中的7条已知基因分别与位于鼠染色体8q31位置的核糖体蛋白、15p12位置的周围肿瘤神经元的抗原(paraneoplastic neuronal antigen)、4p22位置的甘油二酯酶、xq37位置的FMR2蛋白（FMR2 protein）的基因以及以及位于果蝇染色体11p11位置的交叉同族体（roundabout homolog 1）有极高的同源性；有4条基因片段同源性较低，为功能未知的新基因，已经在GeneBank进行了注册，注册号分别CK325391、CK325392、CK325393、CK325394。

The paper discussed how docosahexaenoic acid regulated transcription expression of lipogenic and lipolytic genes, which included PPARγ(peroxisome proliferators activated receptor γ), SREBP-1c (sterol regulatory element binding protein-1c), FAS, HSL (hormone-sensitive lipase) and TGH.

探讨二十二碳六烯酸对小鼠脂肪组织和肝脏生脂相关基因过氧化物酶增殖物激活受体γ、固醇调节元件结合蛋白－1c基因(SREBP-1c)、脂肪酸合成酶基因，及脂解相关基因激素敏感脂酶基因和甘油三酯水解酶时序表达的影响。

The most common methods used for screening of lipase-producing strains are observation of transparent circle on tributyrin agar plate and color-changing circle on olive oil agar plate.

在脂肪酶产生菌的筛选中，最常用的方法是三丁酸甘油酯琼脂平板透明圈法和橄榄油琼脂平板变色圈法。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

该菌株经IPTG诱导可表达分子量约80 kDa的融合蛋白，SDS-PAGE分析表明融合蛋白位于菌体超声裂解后的上清中，用蛋白纯化试剂盒HisTrap~ HP纯化后得到单一的目的蛋白，约80 kDa，且纯化的融合蛋白具有脂酶活性，可以分解三丁酸甘油酯。

These triglyceride-enriched but cholesterol-depleted HDL particles are more prone to be catabolized by hepatic lipase which hydrolyzes phospholipids and triglycerides and reduces the size and density of HDL particles.

这些富含甘油三酯，但缺乏胆固醇的HDL 颗粒更易被肝脂酶分解，使磷脂和甘油三酯水解，HDL颗粒变小且密度降低。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。