甘油三酸酯

To elucidate the possible mechanism of differentiation and/or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor , the level of acetylated histone H3 was detected by Western blot, p21(superscript WAF1) expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin， TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制，利用Western blot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21(上标 WAF1)表达量的改变。

The activity of lipasc from S78- pVT102U/a -lip was up to 20U/ml after 72h fermentation; The product of S78-pVT102U/a-L-lip07 was little, only formed the halo on the tributyrin plate.

其中，重组酵母S78-pVT102U／α-lip摇瓶发酵72h，酶活可达20U／ml；S78-pVT102U／α-L-lip07表达的脂肪酶很少，表达产物仅在三丁酸甘油酯平板上形成透明圈。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

该菌株经IPTG诱导可表达分子量约80 kDa的融合蛋白，SDS-PAGE分析表明融合蛋白位于菌体超声裂解后的上清中，用蛋白纯化试剂盒HisTrap~ HP纯化后得到单一的目的蛋白，约80 kDa，且纯化的融合蛋白具有脂酶活性，可以分解三丁酸甘油酯。

One integrative transformant was obtained through PCR and phenotype identification, designated as A.oryzae ONL1. The 7-day fermentation broth of A.oryzae ONL1 could hydrolyze tributyrin verified by forming transparent cleared zone on tributyrin plate, and the enzyme activity reached 2.5 U/mL. The SDS-PAGE electrophoretic map of the fermentation broth demonstrated that there was a typical RML band at 32.5 kDa.

其培养7天的培养液上清在以三丁酸甘油酯为底物的平板上形成清晰的水解透明圈，碱滴定法酶活测定表明培养液酶活可达2.5 U/mL，培养液上清的SDS-PAGE图谱在32.5 kDa处有RML的特征条带。

The chemistry and technology of epoxidation of oils from silkworm pupae oil rich in linolenic acids, soybean oil rich in linoleic acids and chemical reagent grade triolein rich in oleic acids were systematically analyzed and experimentally studied.

本论文以富含亚麻酸的蚕蛹油，富含亚油酸的大豆油和富含油酸的化学纯三油酸甘油酯为原料进行了油脂环氧化反应的化学与工艺学研究。

Aim To prepare triamcinolone-acetonide-acetate-loaded solid lipid nanoparticles carbomer gel with tripalmitin glyceride, and investigate their characteristics and transdermal drug delivery.

目的以醋酸曲安奈德为模型药物，以三棕榈酸甘油酯为脂质材料制备醋酸曲安奈德固体脂质纳米粒卡波姆凝胶，考察其特性以及药物经皮渗透性能。

Chinensis by the methods of column chromatography (normal and reversed phase silica gel, Sephadex LH-20), preparative TLC and HPLC. On the basis of spectroscopic methods including IR, ESI-MS, 1-D and 2-D NMR, forty eight of them were identified as seventeen flavonoids, seventeen tritepenoids, one cumarin, five steroids and some benzene derivative etc.

中药射干为射干植物的干燥根茎，从中共分离出53个化合物，通过红外、质谱及核磁共振等波谱方法鉴定了包括12个新化合物在内的48个，结构类型分别属于iridal型三萜及其新颖的二聚体、异黄酮、黄酮及黄酮醇、香豆素、甾体、芳香酸和脂肪酸及其甘油酯等。

A novel anionic gemini surfactant,didodecyl-ethoxylate bis-sodium sulfate was prepared in three steps using lauryl alcohol,glycol-epiglyceride and chlorosulfonic acid as raw materials and BF_3-ethyl ether as catalyst.The chemical structure of the product was confirmed by IR spectrum.

以十二醇、乙二醇二环氧甘油醚和氯磺酸为原料，以BF3-乙醚为催化剂，通过三步法制备阴离子型双子表面活性剂双十二烷基乙氧基二硫酸酯钠；红外光谱对其结构进行了表征，测定了其表面活性和发泡力等性能。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。