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Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的:研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性,探讨简便易行的成骨细胞体外培养方法,为组织工程选择理想的种子细胞来源。方法:取新西兰白兔骨髓液经离心后得骨髓单个核细胞,以1× 106/ml的细胞浓度进行培养,经传代培养,选择条件培养液培养(1640完全培养液中加入地塞米松10-8Smol/l,β-甘油磷酸钠10mmol/l,VitC 50μg/ml)作为骨髓基质细胞增殖和成骨的调节因子,通过倒置显微镜、HE染色、扫描电镜、碱磷酸酶(alkaline phosphatase,ALP)测定、Ⅰ型胶原免疫组化染色和四环素荧光钙染色等手段对获得的细胞进行生物学特性研究。

It is postulated that IP induces the release of endogenous protective molecules that interact with cognate receptors to activate PLC through mediation of the chincough virus sensitive proteins. PLC hydrolyzes membrane phospholipid and produces diacylglyccerol,that,by working together with calcium hydronium,translocates PKC from cytoplasm to cell membrane and activates PKC.

推测缺血预处理细胞保护作用机制是:缺血预处理引起内源性保护物质释放,作用于细胞膜上的相应受体,通过百日咳病毒敏感性G蛋白的介导而激活磷脂酶,磷脂酶水解膜磷脂,产生二酰基甘油(diacylglyccerol,DAG),DAG和Ca2+协同作用于PKC,使其从胞浆转位到细胞膜并且被活化,PKC在膜上使底物蛋白磷酸化,引发一系列相关信号转导通路的激活,调控保护性基因的表达,从而提高细胞对缺血再灌注损伤的耐受性。

The present production situation and market prospects of the main epichlorohydrin downstream products,such as epoxy resin,synthetic glycerol, chlorohydrin rubber ,and so on,are described.

论述了环氧氯丙烷的主要下游产品环氧树脂、合成甘油、氯醇橡胶等的生产现状、市场前景等,指出:我国氯碱行业应该重视这些环氧氯丙烷下游精细化工产品的开发,以促进我国环氧氯丙烷的发展。

It can notably lower total cholesterin and glycerinum in serum from mice of hyperlipidemia.

结果表明,决明子正丁醇提取物具有明显的降血脂作用,能显著降低高脂血症小鼠血清总胆固醇和甘油三酯。

The triglyceride level in the lymphocele is not higher than that in serum, which is much higher than that in chyle.

淋巴管瘤中的甘油三脂含量跟血清差不多,但在乳糜池中就很高了。

The volume of drainage, changes of concentration of triglyceride and result of the chyle test were analyzed.

监测腹水引流量及其三酰甘油浓度和乳糜试验的变化。

The rate of chyle transport has been shown to be dramatically affected by the amount of fat, particularly long-chain triglycerides, in the diet.

乳糜流动的速率明显收到饮食中脂肪量尤其是长链甘油三脂的影响。

Results:After the beginning of nutritional intervention, the chyle test turned into negative in short time, and the volume of drainage and the concentration of triglyceride decreased significantly.

结果:4例病人诊断为乳糜腹水并应用营养支持治疗后,短时间内腹水乳糜试验即转为阴性,三酰甘油的浓度显著下降,引流量逐渐减少。

The baseline adjusted area under the curve for chylomicron triacylglycerol was 17% lower after the MC meal (P = 0.02) and 19% lower after the HC meal (P = 0.007) than after the LC meal and 15% lower after the MC meal (P = 0.0495) and 17% lower after the HC meal (P = 0.02) than after the Suppl meal.

与低钙膳食比较,中钙膳食和高钙膳食后的三酰基甘油乳糜微粒曲线下的校正基线面积分别降低17%(P=0.02)和19%(P=0.007),而与补充膳食组比较,则分别下降15%(P=0.0495)和17%(P=0.02)。

The baseline adjusted area under the cure for chylomicron triacylglycerol was 17% lower after the MC meal (P = 0.02) and 19% lower after the HC meal (P = 0.007) than after the LC meal and 15% lower after the MC meal (P = 0.0495) and 17% lower after the HC meal (P = 0.02) than after the Suppl meal.

与低钙膳食比较,中钙膳食和高钙膳食后的三酰基甘油乳糜微粒曲线下的校正基线面积分别降低17%(P=0.02)和19%(P=0.007),而与补充膳食组比较,则分别下降15%(P=0.0495)和17%(P=0.02)。

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