琼脂

METHODS The agar cavity experiment and the experiment of bacteriostatic activity of the guide tube in the nutrient meat broth were made.

用琼脂腔试验法和导管在营养肉汤中的抑菌活性试验测定导管的抗菌性能。

Results The strain grows well in malt wort agar medium,optimized growing temperature is from18to20℃,basid-iospore,pseudohypha and basidium are its characteristic.The results of carbon source assimilation and sugar fermentation are signifi-cantly distinctive,and in accordance with those described in references.

结果 该菌种在麦芽汁-琼脂培养基上生长良好，适宜温度为（20±2）℃，其生长特性为具有担孢子及假菌丝并有芽孢台；碳源同化试验及糖发酵试验均具有特异性，与参考文献所描述的红发夫酵母形态和生化试验结果基本相吻合。

After repeating 5 times, the result shows that the genome DNA can be extracted from the fresh specimens by the methods ,but fail to be extracted from the preserved specimens with the same methods through electrophoresis.

经 5次重复实验，结果表明，用两种方法均能从新鲜标本中获得较完整的DNA ，且DNA得率较高；而对于无水乙醇保存的标本，提取出的DNA用琼脂糖凝胶电泳检测不出DNA条带，说明标本在保存过程中DNA可能发生了变化，致使提取出的DNA量太少或未分离出DNA 。

Methods Determine the marker of hepatitis B by ELISA, total protein by biuret method, albumin by bromocresol green method, and the component of serum protein by agarose gel electrophoresis.

采用酶联免疫吸附实验检测乙肝标志物，双缩脲法检测总蛋白，溴甲酚绿法检测白蛋白，琼脂糖凝胶电泳法分析血清蛋白组分。

Result s:10 μmol/L retinoids could inhibit the Bel-7402 proliferation and decrease the colony forming of liver cancer cells on soft agar significantly.So some biochemical markers indicating the proliferation status of liver cancer cells changed significant ly, including the significant activity increase of ornithine carbamyl transferase, tyrosine-α-ketoglutaric transaminase and alkaline phosphatase, the significant decrease α-fetoprotein secretion,γ-glutamyltranspetidase and aldolase activity.

结果：10μmol/L维甲酸显著抑制肝癌细胞增殖，并使肝癌细胞软琼脂集落形成率明显减少，使代表肝细胞分化的酶鸟氨酸氨基甲酰转移酶、酪氨酸-α-酮戊二酸转氨酶和碱性磷酸酶比活力明显升高，甲胎蛋白分泌量、γ-谷氨酰转肽酶和醛缩酶比活力明显下降。

The expressed products were purified by Glutathion-Sepnarose chelation affinity chromatography and tested by western blot.

对表达的蛋白进行Western blot分析和谷胱甘肽琼脂糖亲和层析纯化，得到纯化的VP4融合蛋白。

A little background: when my grade five was learning about child rights, they were made to roll incense sticks, agarbattis, for eight hours to experience what it means to be a child laborer.

简单介绍一下背景：我们五年级的学生正在学习儿童权益的课程，他们被要求制作庙里用的香，用琼脂二糖，工作8小时，让他们体验到童工的境遇。

And then, the recombinant plasmid was introduced into the component cell E.coli JM109, which transformed by CaCl2 method, and the transformant was selected on M-H agar plate supplemented with 50μg/ml of chloromycetin and 60μg/ml of ampicillin.

将新酶全编码基因克隆入表达载体pHSG398，转化于感受态细胞大肠埃希菌JM109中，用加有抗菌药物的琼脂平板进行转化株筛选。

Then the detection rates of MRS on the three phenotype testing methods were compared with each other. Results A total of 187 clinical staphylococcus isolates were tested including 78 staphylococcus aureus isolates and 109 coagulase negative staphylococcus.

分别采用头孢西丁纸片扩散法、苯唑西林盐琼脂纸片扩散法、Vitek-32 AMS检测187株临床分离葡萄球菌对甲氧西林的耐药性，并比较这三种方法对MRS检出率的差异。

Results indicated that,dominant SSR markers or codominant markers with significant difference on length can be separated by optimized SSR amplification system and high concentration agarose gel effectively.

试验结果表明，优化的SSR扩增体系结合高浓度琼脂糖凝胶能够对显性或差异显著的共显性标记进行高效分离。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。