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Result vitamin A and 4HPR fall to Hela cell collect form have apparent inhibition, can restrain Hela cell to be opposite the action causing tumor of naked rat (the curb tumour action of P.01); vitamin A issues visible cell solid by force to shrink at 4HPR(P.01); report lens, nuclear film is screwy, nuclear chromosome gathers agglomerate stand by the wither such as nuclear film to die DNA of cell of cellular feature; by degradation, a collection of illustrative plates of typical " of shape of " a flight of stairs appears in electrophoresis of agaragar candy gel.

结果维生素A和4HPR对Hela细胞集落形成有明显抑制功能,并可抑制Hela细胞对裸鼠的致瘤功能(P.01);维生素A的抑瘤功能强于4HPR(P.01);电镜下可见细胞固缩,核膜扭曲,核染色体聚集成块并靠近核膜等凋亡细胞特征;细胞DNA被降解,在琼脂糖凝胶电泳中呈现典型的&阶梯状&图谱。

Material and method mop of anus of patient of diarrhoea of appearance of cholera of 1.1 sample origin 3 portion, puke 1. 1.2 reagent are fostered base minim biochemistry is in charge of (epidemic prevention of sanitation of logistics department of Zhejiang province military region examines a), detached agaragar (Jiangsu province sanitation and antiepideic station is microbial reagent factory), diagnostic serum of institute of biological product of Chengdu of Ministry of Public Health. System of APl20E biochemistry appraisal .

ETEC常见散发感染性腹泻,也有报道因ETEC污染水源而暴发腹泻[3],笔者报告的是一起家庭型食物中毒患者肛拭中检出一株肠产毒性大肠埃希菌O6∶K15。1材料和方法1.1样品来源霍乱样腹泻病人肛拭3份、呕吐物1份。1.2试剂培养基微量生化管(浙江省军区后勤部卫生防疫检验所),分离琼脂(江苏省卫生防疫站微生物试剂厂),诊断血清(卫生部成都生物制品探究所)。

The direct amperometric titration of amines with sodium tetraphenylborate solution based upon anodic depolarization at a dropping mercury electrode by the use of excess TPB titrant, was systematically investigated.

本文研究了用pH8的四苯硼酸钠水溶液对胺类的直接电流滴定法。滴汞为阳极,饱和甘汞为阴极,以3%琼脂的饱和NaCl溶液为盐桥组成电解池。适宜的滴定条件是:外加电压+0.18伏;底液为pH 2-3的HAc-NaAc介质,而以1%HAc更为方便;滴定体积为10-20毫升。

Agar is complex polysaccharide, extracted from red algae.

琼脂是一种从红藻中提取的复杂的多糖。

Finally, the constructed β3AR gene antisense expression vector was identified though restriction endonuclease analysis and sequencing. RESULTS: Target gene of about 1.2 kb was successfully cloned into retroviral vector pLNCX. The sequence of cloned β3AR fulllength cDNA was identical to the reported one in GenBank.

结果:经琼脂糖凝胶鉴定,所克隆的目的基因大约为1.2 kb,并成功地连接到逆转录病毒载体pLNCX上,经测序证明,插入的目的片段其碱基序列与Genbank上报道的完全一致,且插入载体的方向完全正确。

Inclusion bodies of expressed proteins were dissolved in denaturants and purified by NTA-Ni gel and refolded under suitable conditions.

用变性剂溶解包涵体蛋白,经Ni-NTA琼脂糖纯化后在适宜的条件下进行重折叠复性。

As support, Agarose can benot only used in normal condition, but also in denaturalization (for example, 6 mol/Lhydrochloric acid carbamidine or 8 mol/L urea). Herein, the preparation of IMACmatrixes were conducted using agarose as support, carboxymethylated aspartate as chelating ligand and Co~(2+) and Ni~(2+) as chelating center ions.

本实验用琼脂糖作为基质,选用四配位,与金属离子有较强作用力的羧甲基天冬氨酸为配基,以Co~(2+)、Ni~(2+)作为螯合金属离子,合成了一系列金属螯合亲和色谱填料,并对其蛋白质分离性能进行了研究。

The soluble fiber fraction accounts for 51-56% of total fibers in green and red algae and for 67-87% in brown algae.

可溶性纤维51-56在绿色(ulvans占总数的一小部分纤维%)和红藻(琼脂,卡拉胶和xylans)和67-87%的褐色海藻。

Injury of CMC of AM group rat was not serious compared with that of BDL group. Conclusion: The results showed that there was the apoptosis of CMC of rat which appeared during OJ, and it was correspondence with the degree of the injury of myocardium.

琼脂糖凝胶电泳观察细胞凋亡特征性DNA梯状带:(1)SO组:无特异条带;(2)BDL组:梗黄6天后即有DNA ladder出现,并随着梗阻时间的延长,带型逐渐清晰、明显。

The present paper reports the phenotype and gene frequencies of GLOI of 7 nationalities in Inner Mongolia province, including the nationalities of Han, Ewenki, Dawuor, Orogen, Buryat,Northeast and Northwest Mongolian, using mixed starch/agarose gel electrophoresis.

应用琼脂糖水解淀粉凝胶平板电泳法,对中国内蒙古呼盟鄂伦春、鄂温克、达斡尔、布里亚特、蒙古人及包头市蒙古族、汉族人血GLOI遗传多态性进行了调查,抽样检测7个群体GLOI表型分布频率,计算出基因频率和识别能力。

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