琼脂

By using electroporation transfects,the TGF〓expression vector were introduced into HL-60 cells.Thecells were allowed to grow for 1 day before beingsubjected to selection for the ability to grow in mediumcontaining 600ug/ml geneticin.Stable cell clones resistantto G418 sulfate were obtained after 2-3 WK growth withdrug-containing medium.Southern blot hybridization wascarried out.RNA slot have higher expression of TGF〓mRNA in HL-60 transfected.All results followed suggestedthat TGF〓 have suppressed the maliganant phenotype of HL-60 cells.

利用电击法将〓表达载体导入HL-60细胞中，经G418筛选培养20天后再行Southern杂交检测外源〓整合情况，利用RNA狭线杂交法证明了在转录水平上表达有所提高，进一步进行了流式细胞仪测定DNA含量，使细胞在〓期产生生长阻滞作用，细胞生长速度，在软琼脂中成集落数、在组织中的浸润性及在裸鼠中的成瘤性均发生变化，我们的实验结果表明TGF〓对HL-60细胞具有抑制恶性表型的作用。

Reverse Transcription polymerase chain reaction with RNA PCR Kit,the product of RT-PCR was determined by electrophoresis of agarose gel (100v, 30s ),the result was shoot by GDS gel imaging system, the staps were scaned and quantitated by computer analysis system.

用RNA PCR试剂盒进行逆转录聚合酶链式反应，RT-PCR产物在1.2%琼脂糖凝胶上电泳(100V，30 min)，用GDS凝胶成像系统拍摄电泳结果，各带用计算机图像分析系统扫描定量。

In this thesis, eight cold-adapted producing bacterium strains were screened from the mud of Parece Vela basin in Pacific Ocean of 5010m deep.

本论文通过琼脂平板透明圈法从太平洋帕里西维拉海盆5010m深的底泥中筛选出八株具备低温脂肪酶活力的菌株，其中的两株生理生化特征相近的菌株D1、D2上清中的酶活力最高，其它菌株所产的酶很大一部分粘附在细胞壁上，上清中的酶活力很小。

To improve the non-biology-stability of navel orange dry wine,the clarification effects of 5 different quantities clarificant s of agar,glutin,diatomite,benotonite and chitosan on navel orange dry wine were compared at zero to 2,40 to 45 centigrade degree and normal temperature,respectively.

为了提高甜橙干酒的非生物稳定性，分别用不同量的琼脂、明胶、硅藻土、皂土和壳聚糖等5种澄清剂对甜橙干酒进行澄清处理，以比较其澄清效果，并分别将甜橙干酒在常温、0~2℃和40~45℃下放置5 d，比较各处理方法对甜橙干酒澄清效果的影响。

The typing antisera of 15 international Haemophilus parasuis reference strains was produced and all Haemophilus parasuis strains isolated from China were serotyped both by Kieletein-Rapp-Gabriedson Gel Diffusion test and Indirect Haemagglutination test.

对本室分离的所有副猪嗜血杆菌按琼脂扩散试验和间接血凝试验血清学分型方法进行血清型鉴定，结果表明我国以血清4型(24.2％)和5型(19.2％)最为流行，其次为13型(12.5％)、14型(7.1％)和12型(6.8％)，另外有12.1％的分离菌株不能进行血清学分型。

Methods: A randomized, single blind controlled clinical design was used to accomplish the series clinical trials included 123 cases. The troop of gatifloxation injection with sodium chloride included 20 cases in gatifloxation group and 23 cases in ciprofloxation group; The troop of gatifloxation injection with glucose included 21 cases in gatifloxation group and 19 cases in levofloxacin group; The troop of gatifloxation pivoxil included 19 cases in gatifloxation group and 21 cases in ciprofloxation group. The antibacterial activity of clinical isolates were detected by scrips which contain gatifloxation(5ug/L), levofloxacin (5ug/L), ciprofloxation (5ug/L), spafloxation(5ug/L), cefotaxime(30ug/L), penicillin(10U/L) respectively. The MICs of sixkinds of antibiotics were detected by 2-fold agar dilution method. Result: The common data of patients in the two groups of 3 troops were similiar.

采用区组分层均衡随机单盲试验设计，分别完成甲磺酸加替沙星氯化钠注射液组试验药20例及对照药23例、甲磺酸加替沙星葡萄糖注射液组试验药21例及对照药19例、甲磺酸加替沙星片剂组试验药19例及对照药21例共123例感染患者的临床试验；用K-B法测定临床分离致病菌对加替沙星(5ug／L)、左氧氟沙星(5ug／L)、环丙沙星(5ug/L)、司帕沙星(5ug/L)、头孢噻肟(30ug／L)、青霉素(10U／L)纸片的敏感性，再用按美国国家实验室标准委员会(National Committee for Clinical Laboratory Standards，NCCLS)推荐的琼脂平皿对倍稀释法测定临床分离菌株对加替沙星、左氧氟沙星、环丙沙星、司帕沙星、头孢噻肟、青霉素的最低抑菌浓度(Minimum Inhibitory Concentration，MIC)以了解其体外抗菌活性。

Methods The Chinese herbal was pureed, and adopted the methods of agar dilution recommended by NCCLS to investigate the MIC of Chinese herbal on the three different kinds of bacteria.

将黄连、黄芩制成煎剂，采用美国临床实验室标准化委员会推荐的琼脂稀释法，测定此2种药物对3种细菌的最小抑菌浓度。

The results showed that Phytophthora sojae on the cellophane carrot agar could not produce oospores within 10 days, no medium were attached when Phytophthora sojae was taken away from cellophane, high-quality RNA, which could meet the requirement of differential display reverse transcriptional PCR was obtained, indicating that it was a suitable cultivation method for Phytophthora sojae to produce pure vegetative mycelia.

结果表明，玻璃纸胡萝卜琼脂培养的大豆疫霉菌，10 d内不会产生卵孢子，当大豆疫霉菌从玻璃纸上取下时，不会带有培养基，能够提取出高质量的RNA，满足差异显示反转录PCR对RNA质量的要求，是一种适合大豆疫霉菌纯营养菌丝的培养方法。

WL nutrient agar ; wine yeast ; sequence analysis

WL营养琼脂；葡萄酒酵母；序列分析

This experiment unified flow cytometry, the monoclonal antibody technology, the cell fluorescence chemistry technology and unilized the union principle of antigen and antibody to examine T lymphocyte subgroup, the B lymphocyte change in circumference blood of the mice through the flow cytometry; At the same time, the agarose gelatin electrophoresis examined bovine colostrum effect on programmed cell death of thymus.

本实验正是将流式细胞术、单克隆抗体技术、细胞荧光化学技术相结合，并利用抗原抗体结合原理，采用流式细胞仪检测了小鼠外周血的T淋巴细胞亚群和B淋巴细胞；同时采用琼脂糖凝胶电泳技术检测了牛初乳对小鼠胸腺细胞凋亡的影响。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。