琼脂

Thymocytes were also pretreated with or without ERKs, JNKs and p38 kinase inhibitors prior to UVB irradiation and DNA fragmentation was assessed by agarose gel electrophoresis. The activation of ERKs, JNKs, p38 kinase were investigated by western-blot.

透射电镜观察细胞超微结构的改变；琼脂糖凝胶电泳观察各组不加入或分别加入ERK抑制剂、JNK抑制剂和P38抑制剂后的DNA ladder；western-blot检测磷酸化ERK、磷酸化JNK、和磷酸化P38的活性。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

Flame atomic absorption spectrometry with standard addition method was used to determine the iron.

目的：建立用琼脂悬浮液进样，标准加入火焰原子吸收法测定奶粉中铁的方法。

Flame atomic absorption spectrometry with standard addition method was used to determine the iron.

以琼脂为悬浮剂，邻苯二甲酸二丁酯为悬浮液稳定剂，将奶粉配制成悬浮液直接进样，用火焰原子吸收标准加入法测定铁含量。

The study describes the antimicrobial activity of garlic essential oil.

采用琼脂扩散法及肉汤稀释法研究了大蒜精油的抗菌活性。

The optimum conditions were as follows: 1 Fe3+/Fe2+ was 1.70-1.75;2 the precipitant,25% ammonia water was used 20%-30%,under 35℃,pH10-11 and with a stirrer speed of 3000 r/min for 1 h;3 in the first package process with gelatin and agar,the optimum amounts was 0.050-0.060g/150mL at the temperature of 55-60℃ and pH of 9-11;In the f...

最佳工艺条件为：(1)Fe3+/Fe2+之比在1.70~1.75；(2)沉淀剂25%NH3.H2O过量20%~30%，温度35℃，pH值11，反应时间为1h；(3)琼脂的最佳用量为0.050~0.060g/150mL；包覆的最佳温度为50~60℃，pH值为9~11；聚乙二醇作为第二次包裹剂时，最佳温度为55℃，反应时间是1h，pH值为4~5；并用透射电镜、分光光度计、古埃磁天平等进行了初步表征。

In Hubei. After screening, two strains of fungal parasites were gottenwhich had stronger. ability to parasite Meloidogyne eggs. Their parasitic ratio tothe root knot eggs was 78.04% and 70.02% in concave slides and water agar plates,72.37% and 65.65% in pots. The effective hatchings in experimental containers wereonly 5.02% and 8.32%, and there were 100% in controls.

危害的根和根围土壤64份样品，采用3种方法分离，经过筛选测定，获寄生能力较强的真菌2株，室内凹玻片和琼脂平板法测定结果表明，对卵的校正寄生率分别为78.04%和70.02%；室外盆栽测定的寄生率分别为72.37%和65.65%，供试卵有效孵化率为5.02%和8.32%，对照卵为100%。4%土重的棉籽壳真菌制剂能有效控制根结线虫第一代卵量，有效用量的10倍菌剂盆栽测定对植物均没有不良影响。

The yield and purity of each DNA sample was tested by UV-spectrophotometer, the integrality of these DNA samples were run electrophoresis on the agarose gel. Each DNA sample was subjected to PCR amplification and hybridization using One lambada rSSO HLA-A,-B and -DRB1 commercial kit, the fluorescent intensity for positive bead and negative bead hybridized with HLA-A,-B and -DRB1 PCR products were calculated and analyzed.

使用2ml深孔板和TECAN DNA全自动工作站，从288份骨髓捐献者样本中提取基因组DNA；提取的DNA样本用紫外分光光度仪测定其浓度和纯度，并采用琼脂糖凝胶电泳检测DNA的完整性；DNA样本用One lambda rSSO HLA-A、B和DRB1基因分型试剂盒进行PCR扩增、分子杂交和Luminex流式磁珠分析仪检测，统计分析每一DNA样本HLA-A、B和DRB1基因扩增产物经DNA探针分子杂交后的阳性磁珠和阴性磁珠的荧光信号强度。

Methods: The minimum inhibitory concentrationof Xipayiguyiy on P.gingivalis,P.intermedia and F.nucleatum was observed by agar dilution in vitro.Also,the effects of Xipayiguyinye on oral malodour challenged by cysteine was investigated on 10 volunteers in vivo.Results: It showed in this study the MIC of Xipayiguyiy for Pg and Fn was 1mg/mL,and for Pi was 0.5mg/mL.It was also found the Xipayiguyinye achieved significant effects on oral malodour challenged by cysteine.

采用琼脂稀释法，测定西帕依固龈液对牙龈卟啉单胞菌（Pg、中间普雷沃菌、具核梭杆菌的最低抑菌浓度；对10例志愿者采用自身前后对照试验，观察四种干预措施（无干预、清水、西帕依固龈液、口泰）对半胱氨酸激发性口臭的最大值水平、各时间点下降百分比及口气值的下降曲线的影响，评价西帕依固龈液防治口臭的效果。

We draw fresh blood with EDTA anticoagulant of two millilitre from every patients. First we draw DNA of leukocyte from blood by the method with saturation phenol and chloroform second we amplificate the ACE gene using the DNA polymerase chain reaction technic employing the primer gene designed by Rigat.

首先应用饱和酚-氯仿法提取白细胞DNA，之后采用Rigat等人设计的ACE基因引物，应用PCR技术对标本ACE基因进行扩增，扩增产物采用2%琼脂糖凝胶电泳。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。