琼脂

Swine enteropathogenic E. colis were rejuvenescenced and 6 of them were selected to prepare blood serum. We had tested the reactance of cross agglutination and agar diffusion between the strains and the blood serums. No. 300 and No. 343 were selected because they had highly cross reaction. To test superantigen of No.300 and No.343, the antigens made by both of them immunized piglets, and then we detected the serum 0D values with indirect ELISA. The OMP and PF of No. 300 and No. 343 were extracted for SDS—PAGE and westen-blotting.

本研究针对10株猪致病性大肠杆菌，经复壮后选取致病性强的6菌株作为供试菌株，制备免疫血清与这6菌株的多种抗原分别进行交叉凝集试验和琼脂扩散试验，根据各菌株抗原的交叉反应程度，筛选出抗原交叉程度高的菌株300和343，检测两菌株超抗原的毒性作用，以不同方式制备抗原免疫仔猪，用间接ELISA检测免疫效果，并提取菌株300和343的外膜蛋白和渗透因子经SDS—PAGE及免疫印迹试验，进行相关分析。

Results According to ability of growth and proliferation of Entamoeba invadens, nutrient agar culture medium containing Ringer's liquor and blood serun is the best among the three kinds of culture mediums.

结果 侵袭内阿米巴在营养琼脂血清盐水培养基中与其它两种培养基比较能好地生长和增殖。

Ethylene Epoxide sterilized agars culture mediums can be used in sampling of clinical treatment and other outburst incident, reducing the preparation time.

环氧乙烷灭菌营养琼脂培养皿可以在一些现场采样中使用，尤其在突发事件的紧急处理工作中，减少了采样的准备工作，可争取宝贵的时间。

Objective To develop a new method for sterilization in agars culture mediums, i.e. Agars culture mediums is sealed to be sterilized and preserved after resolution.

目的：提出了一种培养皿灭菌方法，即营养琼脂培养皿密封后经过环氧乙烷灭菌，解析后保存备用。

Subsequently, the performance of the Pastorex Staph-Plus agglutination test with presumptive MRSA isolates taken directly from chromogenic agars was compared to that of the Pastorex Staph-Plus agglutination test with isolates from blood agar subcultures (conventional_Pastorex agglutination).

随后，我们采用Pastorex Staph-Plus乳胶试剂对显色培养基上被推断为MRSA的菌落进行直接乳胶凝集试验，同时采用接种在血琼脂上进行传代的纯培养菌落进行经典的乳胶凝集试验与之进行比较。

Air sedimentation bacterium was sampled by different methods to analyze the bacterium capture rate. Results Ethylene Epoxide sterilized agars culture mediums bacterium capture rate was similar with regular agars.

结果：营养琼脂培养皿环氧乙烷灭菌后对空气沉降法或对一些表面的采样，收集细菌的效果和培养结果都与普通9 cm玻璃培养皿相当，其结果具有可比较性。

Single clarifier including egg white, agar, gelatin, bentonite and chitosan and composite clarifiers such as bentonite and chitosan were used respectively to clarify wolfiporia cocos fermented wine and their clarifying effects were compared.

采用单一澄清剂蛋清、琼脂、明胶、皂土、壳聚糖和复合澄清剂皂土与壳聚糖对茯苓发酵酒进行澄清研究。

Connective reaction: 6.5μl PCR productions, pMD18-T vector 0.5μl, Ligation SolutionⅠ5μl, T4 DNA Ligase 0.5μl, 10×T4 DNA Ligase 1.5μl were connected overnight at 16℃. 5、transformation of vector: DH5α was prepared by CaCl and mixed with connecters, cultured on Amp+LB overnight at 37℃.

双酶切及PCR鉴定。1%琼脂糖凝胶电泳。7、序列测定：测序结果与Gene Bank中报道的序列同源性为99.1%，说明变链ldh基因及同源区基因已克隆成功；命名该重组质粒为pMD18-T-ldh。

Purified proteinwas dialysed and freezed-dry to inject rabbit preparing rabbit anti-SMOR28 antibody.

利用Ni-NTA琼脂糖提纯表达的目的蛋白SMOR28，SMOR28经透析除盐，冻干浓缩后，免疫兔子制备其抗兔多克隆抗体。

The wheat flour was suspended in agar sol containing dibutyl phthalate and made into suspension.

将面粉悬浮于含有邻苯二甲酸二丁酯的琼脂胶体中而制成悬浮液。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。