琼脂

RESULTS AND CONCLUSION: Two kinds of sulfated agarose had similar degree of substitution and sulfation position, whereas their molecular weight was different.

结果与结论：可以获得取代度及取代位置相似，具有不同相对分子质量分布的硫酸酯化琼脂糖。

Methods The examination specimen with the UuMh liquid media appraisal, the medicine sensitive pore plank performs the medicine sensitive experiment, Simultaneously vaccinates the mycoplasma appraisal agaragar culture medium to make the mycoplasma proof raise.

检测标本用UuMh液体培养基鉴定，药敏微孔板条做药敏试验，同时接种支原体鉴定琼脂培养基做支原体确证培养。

Two of the 9 phenol degrading bacteria were isolated from activated sludge for treating the wastewater from coke industry, while the rest from soil samples.The 2 strains from a coke plant showed one major band of 1.1 kb in ERIC-PCR.

Bruijin用ERIC特异的引物以R.meliloti的染色体DNA为模板进行PCR扩增，发现供试的30多个菌株的产物在琼脂糖凝胶电泳分离时能各自显出独特的条带特征［3］。

Packing with SRB for treating simulative wastewater with sulphate concentration and packing with CSB for treating simulative wastewater withsulfides by activated-charcoal, agar, synthesized sorbent--MagneticHollow porous Polystyrenemicrophere on Upper-flowing Fluidized Bed Reactor, the experiment results show: Magnetic Hollow porous Polystyrenemicrophere characterized with magnetism , large surface area , easier recovering and flowing, of which the reducing efficiency of SRB in Upper-flowing Anaerobic Fluidized Bed Reactor can reach 96 %, the reducing efficiency rate of CSB in Aerobic Fluidized Bed Reactor can come up to 88.4 %, which was the highest among the three sorbents.

本文分别以活性炭、琼脂、及自制的磁性多孔聚苯乙烯微球固定SRB和CSB，在上流式流化床反应器中处理模拟硫酸盐废水和硫化物废水，结果表明：磁性多孔聚苯乙烯微球具磁性、比表面积大、易回收等优点，固定化SRB的SO_4~(2-)去除率高达96％，固定化CSB的硫化物去除率为88.4％。

Results: The growth of NB4 cells was inhibited significantly after treated with berbamine at different concentrations for different time points, the IC50 value was 3.860μg/ml at 48 hours. Morphology analysis showed the characteristics of apoptosis, and the DNA agarose electrophoresis showed the typical DNA ladder. The apoptosis rate increased from 2.83% to 58.44% after treated with berbamine at 12μg/ml for 18 hours. The expression of PML-RARα mRNA presented no significant changes, however, Survivin mRNA was decreased dramatically.

结果：经不同浓度小檗胺处理不同时间后，NB4细胞生长出现明显的抑制，并呈现明显的时间剂量依赖性，48h IC50为3.860μg/ml；细胞形态学观察可见细胞凋亡现象，DNA琼脂糖电泳显示典型的凋亡梯形图：流式细胞仪检测发现细胞凋亡率明显增加，经48h作用后，凋亡率从2.83%增至12μg/ml时的58.44%；虽未发现药物作用后PML/RARα基因表达量的改变，但检测到NB4细胞经药物作用后Survivin基因表达量的减少和Caspase3表达的增加，Caspse3阳性率从对照组的2.06%增至12μg/ml时的70.89%。

METHODS: Pharyngo nasoparyngeal swab specimen were collected from 220 healthy children in two kindergartens in Guangzhou city. The swab was spread on the blood disk, cultured at 35℃, 5% CO2 for 24 h and identified by Optochin paper. The minimal inhibitory concentration of penicillin was measured by the agar dilution method. The positive rates of bacteria carrier were compared by using chi square test.

对广州市区两家幼儿园220例健康儿童，用咽拭子从鼻咽部采集标本接种至血平板，35℃ 5% CO2 条件下孵育24 h，Optochin纸片确诊；琼脂板对倍稀释法测定青霉素最低抑菌浓度。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

In this paper, in order to reduce delamination phenomena of cherry tomato juice in the storage processing. The stability was enhanced and the quality was improved, so as to the long-term stability of cherry tomato juice was achieved. The single factor test and orthogonal test were adopted, and stability effects were studied by some stabilizer such as sodium alginate, carra geenan, edible gelatin, CMC, xanthan gum on cherry.

为了减少樱桃番茄果汁在加工贮藏过程中的分层现象，提高其稳定性，改善其品质，达到使樱桃番茄果汁长期稳定的目的，采用单因素试验和正交试验研究了琼脂、海藻酸钠、卡拉胶、食用明胶、羧甲基纤维素钠、黄原胶等6种常见的稳定剂对樱桃番茄果汁的稳定效果。

A thin film of 1.5% agar was placed over the crystals and allowed to harden, and a cover slip was placed over the agar.

在琼脂边缘加一滴0.1 N HCI。

The resultsshow:(1)Almost all the isolates produced conidia abundently on rice leafpdowder-PDA,corn leaf powder-PDA,and crab grass leaf powder-PDA mediaunder UV-light irradiation.

结果指出，几乎所有的菌株都能在稻叶、玉米叶、马唐草的马铃薯琼脂培养基上经紫外光连续照射，产生大量孢子。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。