琼脂

METHODS: Forty extracted teeth with single straight canals and completed foramina were selected.

选择根尖孔发育完成的单根管离体牙40个，在生理盐水琼脂模型上，分别在根管内灌注25g/L次氯酸钠液、生理盐水、170g/LEDTA、30mL/L过氧化氢液和蒸馏水，在根管干燥条件下，用Root ZX测量牙齿操作长度，分析根管内容物对Root ZX测量值的影响。

Emodin induced the nuclei of K562 with anomalistic and fragmentized for 96 hours. Agarose gel electrophoresis of DNA revealed a typical DNA ladder map of apoptosis.

大黄素作用96h后，荧光显微镜下可见细胞核不规则，核碎裂呈新月形改变，DNA琼脂糖凝胶电泳显示，基因组DNA显现典型的凋亡细胞的梯状图谱。

Gelatin was the first one used as gelling agent for solid media, and then replaced by the agar.

明胶是最早使用的固体培养基凝固剂，已逐渐被琼脂所代替。

Antimicrobial susceptibilities were determined using broth microdilution MIC tests with standard and 100fold higher inocula.

采用琼脂稀释法测定头孢吡肟等5种抗生素在两种接种浓度下的MIC值。

Then the purification of product was ligated into correspondingly restricted pMD18-T vector.

在含有氨苄青霉素抗性的LB琼脂平板上筛选阳性重组子。

The sequences of repeats and anchor nucleates are arbitrarily selected.Coupled with the separation of amplification products on a polyacrylamide or agarose gels,ISSR amplification can reveal a much larger number of fragments per primer than RAPD.It is concluded that ISSR technique provides a quick,reliable and highly informative system for DNA fingerprinting.

重复序列和锚定碱基是随机选择的，扩增产物经聚丙烯酰胺或琼脂糖凝胶电泳分离后，每个引物可以产生比RAPD方法更多的扩增片段，因此，ISSR标记是一种快速、可靠、可以提供有关基因组丰富信息的DNA指纹技术。

Albicans strains with fluconazole. Method: A series of 16 strains C. albicans from the same body were induced to form pseudo-hyphae in RPMI 1640 medium and DMEM medium at 37 癈 for twenty-four hours respectively. The pseudo-hyphae and yeast were counted and the ratio between pseudo-hypha and total cells was calculated. C. albicans strains were induced to form pseudo-hyphae in RPMI 1640 medium for seven days by transferring twelve times. The genome DNAs of the hyphal-form of C. albicans was cleaved by two restriction enzymes EcoR I and Bgl II, the products were electrophored and photoed.

以同一母体来源的白念珠菌CA-1～A-17(共16株)作为研究对象，采用RPMI1640和DMEM培养基，37℃培养24h诱导其假菌丝形成，记录不同观察时间时的菌丝相和酵母相细胞数，计算菌丝相白念珠菌形成率；以RPMI1640培养基，37℃连续传代培养7天(转种12次)后，分离菌丝相白念珠菌并提取其基因组DNA；以EcoRⅠ和BglⅡ消化菌丝相基因组DNA，消化产物进行琼脂糖凝胶电泳并拍照；用随机引物(5'…TCACCACGGT…3')扩增菌丝相基因组DNA，采用变性聚丙烯酰胺凝胶电泳分离扩增产物并拍照。

Methods HOS cell line was treated with various concentrations of tretinoin in different durations in vitro. Proliferation suppression was observed by MTT method and invert microscope before and after tretinoin treatment, and protracting cell growth curve. The cell growth and invasion ability was measured with the colony-formation rate in soft agar test.

不同浓度的维甲酸作用HOS细胞，采用噻唑蓝法、倒置相差显微镜观察药物对细胞生长的影响并绘制细胞生长曲线；软琼脂集落形成实验观察细胞生长和侵袭能力的变化；流式细胞仪测定细胞周期变化。

The sample was treated with suspension technique. Turnip sample was crushed and sieved to make agar suspension.

用悬浮液技术处理萝卜样品，将样品粉碎、过筛，制备成琼脂悬浮液。

OBJECTIVE: To study the anticoagulant activity of the sulfated agarose with different relative molecular masses.

目的：观察不同相对分子质量硫酸酯化琼脂糖的抗凝血性能。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。