琼脂

Methods the anti-inflammatory effect of omi was determined on three inflammatory models: swelling of mice ear induced by dimethylbenzene, hind paw edema and back granuloma caused by hypodermic injection of agar in rats. the an- algesic action of omi was studied by observing the mice's reaction to the heat simulation. the effect of omi on bleeding time was tested by tail-cutting method.

制备二甲苯致小鼠耳部炎症、琼脂致大鼠足肿胀急性炎症模型，大鼠琼脂肉芽肿慢性炎症模型，观察口冰对炎症的作用；采用热板法进行小鼠热刺激性疼痛试验，断尾法测定小鼠出血时间，观察口冰对小鼠疼痛及出血时间的影响。

Colonies on improved agar, when grown 24～48 hours at 37℃ in a candle jar, are 1～2mm in diameter, convex, entire, transparent, glistening, and butyrous. Some strains produce slimy growth. Colonies on clear media are iridescent when observed with obliquely transmitted light.

生物学特性研究表明，该菌在普通营养琼脂平板上不生长，在改良琼脂平板上，烛缸中，37℃培养24～48h后可形成微凸起、边缘整齐、透明有光泽的奶油状光滑型菌落，无溶血现象，菌落无色素，直径1～2mm，在麦康凯培养基上不生长。

The research displayed that yeasts Genus level can be discerned by agarose gel electrophoresis and polyacarylamide gel electrophoresis, but the latter is more advanced. The Saccharomyces cerevisiae strains ITS-1 sequences are most disalike, few of them are equal completely. The bigger differences are part of base pairs about Poly-T, others are exchanged by single base pair.

研究表明，以酵母菌株的rDNA ITS-1序列进行琼脂糖凝胶电泳，能把菌株分类鉴定到属的水平；采用非变性聚丙烯酰胺凝胶电泳能把部分酿酒酵母菌株分类鉴定到种的水平，区别效果较琼脂糖凝胶电泳有所提高；对rDNA ITS-1序列进行克隆、测序，经序列比对后发现，酿酒酵母属中酿酒酵母菌株的ITS-1序列大多数有差异，差异主要表现在ITS-1序列上游的一段Poly-T碱基上，其它处的差异仅是个别碱基的转换或颠换，ITS-1序列长度分布在360-370bp之间，G+C%含量均在35.3%左右。

Methods The plasmid pCEP4P53 expressing human p53 protein and the plasmid pCEP4ASCMH expressing human antisense c - myc gene in eukaryocyte were co - transfected to human bladder cancer cell T24 strain using FuGENE6 as a media, and the results were analyzed by MTT method, cell colony - forming test in soft agar medium and agarose gel electrophore...

将可在真核细胞表达P53蛋白的质粒pCEP4P53和表达反义c-myc基因的质粒pGEP4ASCMHC以染试剂Fu-GENE6为介导，同时转染到入膀胱癌细胞T24中，经MTT检测、软琼脂集落生长、琼脂糖电泳等方法分析实验结果。

Induced by 4℃ low temperature, Escherichia coli inoculated in water can enter the viable but nonculturable state; in the room temperature, Escherichia coli may keep culturable state for a long period; eutrophic culture media of TSA can produce more bacteria count compared with oligotrophic culture media of PCA; evaluating the bacteria count Escherichia coli in water by plate count method may underestimate the risk of intestinal pathogen contamination.

在4℃低温诱导下，大肠埃希菌在水中可进人活的非可培养状态；在室温下，大肠埃希菌在水中可保持较长时间的可培养性；富营养的TSA琼脂检测的菌落数多于寡营养的PCA琼脂；用平板涂布法检测水中的大肠埃希菌可能低估了肠道致病菌污染的风险。

Root,immunoassay of ELISA(enzyme linked immunosorbent assay) was employed to determine IAA concentration in root tip, unilateral application of agar block containing IAA on root tip was carried out to investigate the effect of exogenous IAA on phototropic response.

为了研究生长素在水稻根负向光性反应中的作用，用含有IAA的琼脂块贴附在种子根的根尖表面观测IAA对根的生长效应，用ELISA法测定根尖的IAA含量，并对种子根弯曲部分进行半薄切片，结果表明：(1)种子根的生长方向既受光的调控也受外施的IAA的调控，根尖向贴有含IAA琼脂块的一侧弯曲生长；(2 )不定根在光照 1.5h后，背光侧的IAA含量明显大于向光侧；(3)种子根尖发生弯曲生长是伸长区的细胞不均等生长所致。

The c oaventioaal method was improved by using agarose as supporting medium instead pured agar and adopting pre-electrophoresis in order to make it more sensitive.

笔者在常规方法的基础上，做了些改进：用琼脂糖代替纯化琼脂；并先将诊断血清加在阳极端孔内，预电泳15—20分钟，然后将待检标本加入阴极端孔内继续电泳50分钟。

Methods Modified Columbia chocolate agar was compared with blood agar staphy stick for the primary isolation of Hi from sputa. The sensitivities of 20 antibiotics to Hi were determined in vitro by KB method and MICs of 12 antibiotics were determined by broth microdilution method. The clinical characteristics of 92 cases with lower respiratory tract infection by Hi were analyzed.

比较改良哥伦比亚巧克力琼脂与血琼脂加金黄色葡萄球菌划线对Hi的分离率；以Kribry-Bauer法测定Hi对20种抗生素的敏感性，以肉汤微量稀释法测定12种抗生素对Hi的最小抑菌浓度；分析92例Hi下呼吸道感染的临床特点。

The most common methods used for screening of lipase-producing strains are observation of transparent circle on tributyrin agar plate and color-changing circle on olive oil agar plate.

在脂肪酶产生菌的筛选中，最常用的方法是三丁酸甘油酯琼脂平板透明圈法和橄榄油琼脂平板变色圈法。

The results show that both different banana-attractants and sugar vinegar-attractants had obvious attractive effect to the pest. Among them, banana-attractant (banana : agar solution : honey : vinegar in a ratio of 100:100:30:10) and sugar-vinegar attractant (brown sugar : agar solution : alcohol : vinegar in a ratio of 35:100:30:50) provided a better attractive effect than other analogous attractants, with killing percentages of 77.79 and 94.78, respectively.

结果表明，香蕉诱剂和糖醋诱剂对黑腹果蝇成虫都具有明显的诱杀效果，其中香蕉诱剂配方（香蕉肉∶琼脂水∶蜂蜜∶醋的比例为100：100：30：10）和糖醋诱剂配方（红糖∶琼脂水∶白酒∶醋的比例为35：100：30：50）的诱杀效果比其他配方更好，平均诱杀效果分别可达77.79%和94.78%。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。