琼脂

Morphology of the EPCs was observed, and the growth curve of the EPCs was investigated. Surface antigens of the EPCs were analyzed by the flow-cytometer. The capability of intaking the acetylated low-density lipoprotein of the EPCs was detected using fluoresencent chemical method. The vasoformative capability and genetic stability of EPCs were cultured in matrigel, and examined by karyotype analysis.

观察培养细胞的形态，测定其生长动力学，采用流式细胞仪及免疫细胞化学方法检测其表型，应用细胞荧光化学法分析EPCs对乙酰化低密度脂蛋白(acetylated low-density lipoprotein， ac-LDL)的摄取功能，Matrigel上培养并检测其形成血管能力，染色体核型分析其遗传稳定性，裸鼠实验、软琼脂实验观察致瘤性。

The iso-electric point of was 4.8-5.2. The amino acid analysis indicated that the recombinant protein is non- N-glycosylated.The ability of 0.5 ug/m rhsTRAIL to induce apoptosis of liver cancer cells SMMC7721 and BEL 7402 could be visioned morphologically. This was further verified by Electrical microscope observision of induced SMMC7721 cells and agarose gel electrophoresis of apoptosis DNA. MTT test results also showed that 0.01 u g/ml purified rhsTRAIL has strong effect to induce apoptosis of SMMC7721 cell.

形态学上观察了rhsTRAIL对肝癌细胞SMMC7721和BEL7402的诱导凋亡活性，发现rhsTRAIL在0.5μg/ml时显示了很好的诱导凋亡活性，并通过电镜观察rhsTRAIL诱导后的SMMC7721细胞，证实了凋亡；同时提取凋亡细胞DNA，进行琼脂糖电泳分析，在分子水平证明凋亡；最后利用SMMC7721细胞分组加入纯化后的rhsTRAIL蛋白，结果显示纯化蛋白含量在0.01μg/ml时细胞就呈现了诱导凋亡活性。

A screening and cultivation method for Bacillus thuringiensis of high-yield high temperature resisting protease includes the following steps: high-yield high temperature resisting protease Bacillus thuringiensis Bt140 is obtained through adopting casein substrate agar to identify flat primary screen and fermenting enzyme activity measuring secondary screening; Plackett-Burman design and response surface analytical method are adopted to obtain optimal fermentation zymogenic formula and fermentation condition for Bt140 quickly; finally, shaking fermentation level reaches to 918.56U/mL.

一种高产耐高温蛋白酶的苏云金芽孢杆菌筛选及培养方法，采用酪蛋白底物琼脂鉴定平板初筛与发酵酶活测定复筛的方法获得高产耐高温蛋白酶苏云金芽孢杆菌Bt140；采用Plackett-Burman设计与响应面分析法快速获得了Bt140最佳的发酵产酶配方与发酵条件，最终摇瓶发酵水平可达918.56U/mL。

Methods Polymerase chain reaction was used to detect HPV 16 DNA and point mutation at condon 12 of Ha-ras oncogene.

应用多聚酶链反应技术扩增HPV16 DNA和Ha-ras癌基因相关片段，分别采用2％琼脂糖凝胶电泳和限制性片段长度多态性分析，检测口腔癌中的HPV16 DNA和Ha-ras癌基因第12位密码子的点突变。

The frequencies of transformation of HLF cell exposed to β-carotene and vitamin C were decreased significantly. The cells could not be agglutinated by ConA and not grow in semisolid agar.

添加β-胡萝卜素和维生素C的处理组比单用Ni2O3组的转化率显著下降(P＜0.05)，处理后细胞ConA凝集反应阴性，不能在软琼脂内生长。

The transformed cell could be agglutinated by lower concentration of ConA and could grow in semisolid agar.

为了探讨三氧化二镍(Ni2O3)诱导人肺成纤维细胞恶性转化作用以及β-胡萝卜素和维生素C的保护作用，用不同浓度的Ni2O3在体外多次处理HLF细胞，利用刀豆凝集素A凝集实验和软琼脂集落形成实验进行转化细胞的恶性鉴定；同时添加β-胡萝卜素(5.4mg/L)和维生素C的食物(1.8mg/L)，观察对Ni2O3转化作用的影响。

The agar diffusion and plate serial dilution test was used to detect the effect of CMCT-Ag+ against Pg by observing cingula diameter and bacteria plaque.

采用琼脂扩散和固体平板二倍稀释法观察抑菌环直径和菌斑数，测定 CMCT-Ag+对 Pg的抑制作用。

To investigate the inhibitory patch of nickel, we analyzed intermediates of limiting repair reaction by denaturing gel electrophoresis.

所以为了进一步探讨镍抑制人类核酸配对错误修复系统的路径，我们利用了碱性琼脂凝胶分析修复反应的中间产物。

In proof of this interpretation, examples are quoted from scripture, as:"The seven kine are seven years"(Genesis 41:26) or:"Sara and Agar are the two covenants"(Galatians 4:24), Waiving the question whether the verb "to be" of itself can ever be used as the "copula in a figurative relation" or express the "relation of identity in a metaphorical connection", which most logicians deny, the fundamental principles of logic firmly establish this truth, that all propositions may be divided into two great categories, of which the first and most comprehensive denominates a thing as it is in itself (eg "Man is a rational being"), whereas the second designates a thing according as it is used as a sign of something else (eg,"This picture is my father").

在证明这一点的解释，例如引述经文为：&七凯恩斯是七年&（创41：26 ），或者：&萨拉和琼脂是两个人权公约&（加拉太4时24分），免收问题是否动词&，以&的本身都不能被利用为& Copula的一种形象化的关系&或表述&的关系，身份，在一个隐喻性联系&，其中最logicians否认的，基本原则的逻辑，牢固树立了这个真理，即所有的主张，可分为两个大类，其中第一和最全面的denominates一件事情，因为这是在自己（如：&人是一种理性的&），而第二指定事据，因为它是用来作为标志的东西，别人（例如，&这张照片是我爸爸&）。

Extracted DNA by CTAB, in proportion to the additions of PVP and β-mercap toethanol to the extraction buffer were capable of preventing browning from taking place in the extraction, Tris-Phenol (Stabilized with 0.1M Tris, pH 8.0)/Chloroform/isoamyl alcohol were used to remove protein and impurity in the second extraction, during crude DNA used to Isopropanol DNA and deterged by DNA detergent, without using RNase remove RNA, we detected the quality and concentration of DNA respectively for normal leaves and drought stress leaves by Agarose gel electrophoresis of genomic DNA and ultraviolet spectrometry photo.

用20%聚乙二醇(polyethylene glycol，PEG6000)模拟干旱，采取CTAB提取缓冲液，按一定比例加入PVP和β-巯基乙醇，用Tris平衡酚－氯仿－异戊醇除去蛋白质及杂质，在粗提时用异丙醇沉淀DNA，并用DNA洗液（10mmol/L醋酸铵，75%乙醇）洗涤DNA，在纯化时用乙醇沉淀DNA，且没有加入RNase去除RNA的方法，分别提取2个品种正常和干旱胁迫的苹果属叶片总DNA，并对提取的DNA进行琼脂糖凝胶电泳和紫外分光光度计检测。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。