琼脂

Adding purified stylar S-RNase from Prunus mume cultivar 'Gessekai' to liquid medium, for cultivating self- and cross-pollen at temperatures lot, 25℃ and 35℃, respectively, then determining the rate of pollen germination and the length of pollen tube after different periods of cultivating time.

在液体培养基中添加从果梅'月世界'花柱中纯化的S-RNase，并分别于10℃、25℃和35℃条件下培养自花、异花花粉，测定不同培养时间后的花粉萌发率和花粉管生长长度；离体条件下进行果梅花柱S-RNase降解花粉管RNA反应后，用琼脂糖凝胶电泳检测不同温度条件下自花、异花花粉管RNA被降解的程度。

To evaluate the LDL particle size and subfraction pattern and their association with coronary heart disease in Chinese population, a case-control study was conducted. Using the agarose-acrylamide composite gradient gel electrophoresis, LDL peak particle diameter was determined in 105 subjects with angiographically verified coronary artery disease and 103 matched healthy controls. Two LDL subfraction patterns were identified by the LDL peak particle diameter.

本课题以病例对照研究设计评估国人LDL颗粒大小和亚组分模式及其与冠心病关系。105例经冠状动脉造影确诊的冠心病患者和年龄性别构成匹配作为对照组的103例健康检查者的LDL峰颗粒直径，用琼脂糖聚丙烯酰胺混合凝胶电泳进行测定。

Here, by introducing C-terminally 6xHis-tagged human Nap1 gene into Escherichia coli and exploiting the nickel-charged sepharose gel system to purify the human Nap1 protein, we show that the Nap1 enzyme function is preserved in human as in lower organisms such as bacteria and plants using succinamic acid as the substrate.

此研究中，我们将已在胺基末端接上6个组胺酸标记的Nap1基因表现在大肠杆菌中，并使用接合上镍原子的琼脂糖凝胶纯化出人类Nap1蛋白，再以丁醯胺酸作为受质，证明人类Nap1蛋白具有酵素活性。

Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例，分成三份，分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本，用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA，以RT-PCR方法扩增β-Actin基因，经1.5%琼脂糖凝胶电泳，溴化乙锭染色，紫外透射仪下观察分析。

Methods One hundred thirty PCOS women and one hundred seventy five normal women as controls were enrolled in this study. The genotypes were screened by polymerase chain reaction-On/off switch and the product was isolated by electrophoresis on a 2.5% agarose gel containing ethidium bromide and visualized using an ultraviolet transilluminator.

应用On/off Switch-聚合酶链反应及琼脂糖凝胶电泳分离技术分析130例多囊卵巢综合征患者及175例对照组妇女TNF-α基因rs3179060C/A多态，比较rs3179060C/A两组等位基因及基因型与PCOS及其高雄激素之间的关系。

Methods The genomic DNA extracted from the 2 trematodes was amplified with random amplified polymorphic DNA and then was carried on agarose gel electrophoresis.

应用随机扩增多态性DNA（Random amplified polymorphic DNA，RAPD）技术对斯氏肺吸虫和华支睾吸虫的基因组多态DNA进行扩增，扩增产物经琼脂糖凝胶电泳后分析结果。

Argrose electrophophoresis and UV detection showed that undegenerated and high purified DNA from feather was fit to the detection on gene level.

琼脂糖电泳及紫外检测均证明，从羽毛中提取的DNA分子无降解，纯度高，适合于基因水平的检测。

A study was done to isolate and identify yeasts from apple, which (15 samples) were collected from the North Andre Apple Juice Co. Ltd. The yeasts were isolated with unpasteurized samples in the media of glucose-yeast extract-tryptone media. The identification of yeasts were done with the physiological characteristics and the API 20C AUX system. The Candida spp., Kloeckere spp. and Saccharomyces spp.

利用酵母粉－葡萄糖琼脂培养基对15个批次苹果原料样本中的酵母菌进行分离，并用API20C AUX菌种鉴定系统进行鉴定，得到的25株酵母菌分别属於假丝酵母菌属、酿酒酵母菌属和克勒克酵母菌属的6个种：克柔／平常假丝酵母菌 13株，酿酒酵母菌 5株，克勒克酵母菌属酵母菌Kloeckera spp。

The uropoiesis genital tract mycoplasma infects the Uu masculine gender rate to be highest, the feminine masculine gender rate is highest; The mycoplasma medicine sensitive first choice medicine is the force mildew element, junction Sha Meisu, the content mildew element; Must use the mycoplasma appraisal agaragar culture medium to make the mycoplasma proof raise.

泌尿生殖道支原体感染Uu阳性率最高，女性阳性率最高；支原体药敏首选药物是强力霉素、交沙霉素、美满霉素；必须使用支原体鉴定琼脂培养基做支原体确证培养。

To mensurate the titer of the specimen, this method compares size of the bacteriostatic rings to the vaccinal test organisms produced by the standard and the tested by using the diffusion effect of antibiotics in agar culture medium.

本法是利用抗生素在琼脂培养基内的扩散作用，比较标准品与供试品两者对接种的试验菌产生抑菌圈的大小，以测定供试品效价的一种方法。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。