琼脂

Using of agarose-embedment lysis cell wallmethod for preparing intact chromosomal DNA from phytopathogenic fungi is thefirst time.

首次采用凝胶包理破壁法由植物病原真菌分生孢子获得完整染色体DNA，克服了先用裂解酶破壁获得大量原生质体再用琼脂糖包理原生质体的传统制备方法的一些不足。

The plasmodial type is the phaneroplasmodium which appears as a fan-shaped network of veins.

琼脂培养基上获得的细弱绒泡菌孢子与野生型相似，并具有可育性。

Thirty specimens samples were randomly divided into three groups blank control,media control and inoculated m edia group, 10 specimens were used in each group under the aerobic conditions,Sa polos glucose agar media with candida albicans inoculated .

需氧环境将白色念珠菌接种到沙保罗葡萄糖琼脂培养基后，将试件贴附其表面每周转换传递1次，共10周。0.5g/L戊二醛消灭细菌清洗，2周后，用Minolta CR-100 型色度计观察。

The optimal way for inducing floral buds is to preculture the plantets on 1/2MS medium supplemented with 0.5mg/L ABA and 0.5mg/L PP333 for 35 days, then transferred them onto 1/2MS medium supplemented with 1.0mg/L PP333 and 0.1mg/L TDZ. The frequency of floral buds induction increased sharply reaching 62.2% during a period of 150 days. All the mediums were added to 30g/L sucrose and 9g/Lagar.

通过筛选，诱导金钗石斛花芽形成效果最好的培养方法是：以1／2MS附加蔗糖30g／L，琼脂9g／L，活性炭0.5g／L为基本培养基(pH5.6～5.8)，将组培苗在加有PP_(333)0.5mg／L+ABA0.5mg／L的培养基上预培养35d后，再转入到附加TDZ0.1mg／L+PP_(333)1.0mg／L的培养基中。150d内统计的花芽形成率高达62.2％。

Prepare a mixture having the same composition as that of the Fluid Thioglycollate Medium , but omitting the agar and the resazurin sodium solution, sterilize as directed above, and allow to cool prior to use.

配制与巯基醋酸盐液体培养基成分相同，但省略了琼脂和刃天青钠溶液的混合物，按上述方法灭菌，并在使用前静置至凉。

MethodsEthanol extract of Rhynchosia volubilis Lour roots was applied in vitro in spermicidal tests with 10 mouse sperm samples and 10 human sperm samples.

用试管法及琼脂平皿稀释法观察鹿藿醇提取物对几种临床常见致病菌的体外目的研究鹿藿醇提取物的体外杀精及抗菌作用。

The antibacterial effects of ethanol extract of rhynchosia volubilis lour roots on staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, and pneumobacillus were observed.

用试管法及琼脂平皿稀释法观察鹿藿醇提取物对几种临床常见致病菌的体外抑菌作用。

Using the MTT assay, agarose gel electrophoresis, transmission electron microscope, TUNEL method and immunohistochemistry (PCNA, bcl-2),it was studied in this experiment that the inducing APO, anti-proliferation and affection of expression of bcl-2 gene before and afterAPO of the Rubia Cordifolia L with the 5-fluorouracil(5-fu) for MGC-803 cells of gastric carcinoma.

本实验通过MTT法、琼脂糖凝胶电泳法、透射电镜观察法、TUNEL法、免疫细胞化学法（PCNA、bcl-2），研究了茜草提取物联用5-氟尿嘧啶（5-fluorouracil，5-Fu）对胃癌MGC-803细胞株的诱导凋亡、抗增殖作用及凋亡前后bcl-2基因表达的影响。

Methods Four pairs of these primers that would be amplified in simulium COI gene were designed by Primer Premier 5.0, the results of PCR would be investigated through agarose gel electrophoresis.

运用Primer Premier 5.0软件设计4对蚋科COI基因的PCR扩增引物，通过PCR扩增后进行琼脂糖凝胶电泳检测实验。

Spreng was much more simple and efficient than the conventional methods. And the extracted DNA was suitable for the polymorphic analysis based on PCR, such as ISSR-PCR.

通过琼脂糖凝胶电泳和OD260/OD280比值测定，所得基因组DNA纯度高，可作为ISSR等以PCR为基础的DNA多态性标记。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。