琼脂

Methods: The minimum concentration inhibitory to Neisseria gonorrhoeae of ceftriaxone was determined by using the agar dilution method.

采用琼脂稀释法测定头孢三嗪对淋病奈瑟菌的最小抑菌浓度(minimalinhibitoryconcentration ，MIC)。

Methods After ZTO injection acted on human ovarian cancer SKOV3 cells, the changes in the nucleus were observed by fluorescence microscope, oncosis index was counted by projection electron microscope, the situation of cell DNA breakage was observed by agarose gel electrophoresis, and cell Bcl-2 gene expression was observed by immunohistochemical method.

以不同浓度莪术油注射液作用人卵巢癌SKOV3细胞后，通过荧光显微镜观察细胞核变化、透射电镜计算细胞胀亡指数、琼脂糖凝胶电泳观察细胞DNA断裂情况、免疫组化观察细胞Bcl-2基因的表达。

After being digested with EcoR I and Xho I, they were inserted to the vectors pGEX-4T-l orientally, and then transformed into E.coli BL21(DE3) cells. The monoclone were selected and identified by means of restriction analysis, PCR and sequencing. As the result, the two recombinant plasmids with the different DNA fragements of the PrP gene were constructed and designated as pMY01[Ov rPrP(23-244)] and pMY02[Ov rPrP(91 -244)], respectively.

挑选单个菌落，提取重组质粒，经酶切、PCR扩增后琼脂糖凝胶电泳分析和测序鉴定，成功地构建了2种绵羊重组朊蛋白的表达质粒pMY01[OvrPrP(23～244)]和pMYO2[Ov rPrP(91～244)]。

The cells ovoidal, while budding the wall structure is entroblastic. Reproduction by multilateral budding. It is not form ascospores or ballistospores; on malt agar red carotenoid pigments is synthesized.

该菌株细胞卵圆，出芽时胞壁结构为内分芽型，多端芽殖，不产生子囊孢子和掷孢子；在麦芽汁琼脂上产生红色胡萝卜素，颜色反应阳性，菌落平滑，有光泽，质地软而呈粘液状，不发酵任何糖，能同化麦芽糖、蔗糖、乳糖、棉子糖、松三糖和硝酸钾，能分解尿素；细胞水解物中含甘露糖、葡萄糖和半乳糖。

The flavonoid substance being in extract of cyclocarya paliurus possesses the functions of reducing blood sugar, reducing blood pressure and reducing blood fat.

青钱柳提取物中类黄酮等物质有降血糖、降血压、降血脂功能，将青钱柳提取物，配以风味剂，辅助物质，并加入海藻酸钠、羧甲基纤维素、琼脂、黄原胶中两种或两种以上物质作为稳定剂，可以较长久地保持青钱柳提取物中降血糖、降血压、降血脂物质的稳定性。

Fig1. Effect of temperature on mycelial growth of Pestalotia psidii, Colletotrichum gloeosporioides, Phomopsis sp.

图一、度对于五种番石榴果腐病菌的菌丝在马铃薯葡萄糖琼脂培养基生长的影响。

Apoptosis induced by boanmycin in human nasopharyngeal cancer SUNE-1 cells was tested by fluorescent microscope, flowcytometry and DNA gel electro phoresis.

应用荧光显微镜、流式细胞仪及琼脂糖凝胶电泳等方法检测博安霉素诱导人鼻咽癌SUNE-1细胞凋亡的作用。

The Charon library DNA was then digested with restriction enzymes BamH Ⅰ and Hind Ⅲ, and DNA fragments of length of about 0.5-1. 5kb was subcloned into plasmid pUC 18 which was also digested by BamH Ⅰ and Hind Ⅲ and dephosphorylated by alkaline phosphatese before being ligated with Charon fragments.

抽提λCharon文库总DNA，用BamHⅠ和HindⅢ完全酶切电泳，用低熔点琼脂糖回收长约0.5-1.5kb的DNA片段；连接到pUC18上，pUC DNA先用BamHⅠ和HindⅢ酶切，后用碱性磷酸酯酶脱磷。

Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells; photomechanical method used to observe configuration and characteristics of apoptosis cells; FCM employed to test the percentage of apoptosis cells; electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.

采用四甲基偶氮唑盐显色法，检测不同浓度5－FU作用不同时间对Lovo细胞生长所产生的不同影响；光镜观察凋亡细胞的形态特点；流式细胞术检测凋亡细胞百分率；琼脂凝胶电泳、免疫组化SP法检测 Lovo细胞增殖核抗原Ki－67及凋亡相关基因蛋白p53、Fas表达水平。

The inhibitory activity of Echcin to 14 strains of phytopathogenic bacteria was tested using paper plate method.

采用纸碟法测定了Echcin 对5 个属植物病原细菌共14 个菌株的抑制作用，琼脂稀释法测定了Echcin 对12 种植物病原真菌的抑制作用，在此基础上开展了Echcin 对番茄早疫病和稻白叶枯病的温室盆栽试验，同时开展了Echcin 对小麦纹枯病的田间小区试验。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。