琼脂

High proportion of cells was in Sub-G1 phase of cell cycle and the cells could not grow in soft agar to form colonies. On the contrary, the three breast cancer cell lines, Bcap-37, MCF-7 and MDA-MB-231, are anoikis resistant, as indicated by DNA laddering and FCM assays.

而Bcap-37、MCF-7、MDA-MB-231三种乳腺癌细胞则具有抗脱落凋亡的特性，分别与它们各自贴壁培养的细胞比较，胞浆DNA琼脂糖凝胶电泳表现无明显差异，FCM所检测到的凋亡峰也无明显区别。

The results indicated that DNase E belonging to endonuclease.Conclusion1. Established a preparation technics of rude extracts of Lumbricus Bimastus nucleases.

应用琼脂糖凝胶电泳对DNase E水解双链环状DNA后的产物进行鉴定，证明了DNase E属于核酸内切酶。

Methods1. Preparation of rude extracts of Lumbricus Bimastus nucleases and its purification Using DNA-casting SDS-PAGE and agarose gel to monitor the activity of the nuclease in the process of extraction.

双胸蚓组织中核酸酶粗提物的制备及核酸酶的纯化以SDS-PAGE-DNA功能胶和琼脂糖凝胶电泳测活相结合作为双胸蚓组织核酸酶提取过程中核酸酶活性的监测体系。

A preliminary study on substrate specificity of Lumbricus Bimastus nucleaseTo measure degrade rate of DNase E to several different substrate in optimal reaction condition to demonstrate the substrate selectivity of DNase E. Agarose gel electrophoresis was used to investigate the products of DNase E processing double strands cyclic DNA to investigate the action of DNase E.

双胸蚓组织核酸酶底物特异性的研究测定DNase E在最适反应条件下对不同底物的降解速率，证明DNase E降解底物的选择性；应用琼脂糖凝胶电泳对DNase E水解双链环状DNA后的产物进行鉴定，阐明DNase E的水解方式。

Schistosoma japonica antigen cDNA clones were identified by lysogenic expression,flat lyiic method and PCR amplification. All 8 positive clones immunologically screened could be expressed in E- coli in the form of fusion proteins with the molecular weight being about 140 to 150 kDa. The positive cDNA genes were digested by restriction endonuclease EcoRI,then the agarose gel electrophoresis revealed the size of them being 700 to 900 bp.

利用融源表达、平板裂解法和PCR扩增三种不同方法分别对日本血吸虫抗原cDNA基因进行鉴定和分析，8个免疫筛选阳性克隆均能在大肠杆菌中以融合蛋白的形式表达，表达蛋白分子量为140～150kDa，抗原cDNA基因经限制性内切酶EcoRI酶解后，琼脂糖凝胶电泳显示其大小为700～900bp，PCR能扩增出特异性条带。

Among them strain MZ1 had been confirmed a lysogenic strain by indicator strain ZK1 after producing phage plaques induced by Mitomycin C.Two templates of overnight cultivation and its DNA from strain MZ1 were then amplified in the same time with five pairs of primers designed from the same phage,and the same products were obtained,suggesting that strain MZ1 was also confirmed a lysogenic strain by PCR method.

MZ1菌株的过夜培养液与指示菌ZK1(血清型H25)混匀并在双层琼脂上培养12 h后出现了滴度为2×107 pfu的噬菌斑，从而证明生产菌株MZ1确为溶原菌；又以GIL01同源性较高的其它四个基因设计引物，分别以MZ1的培养物及其溶原性噬菌体基因组为模板进行PCR扩增，结果两者得到几乎一致的带型，进一步证明了生产菌株MZ1为溶原菌，说明利用PCR法检测鉴定苏云金杆菌的溶原性是可行的。

Analysis and fitting of growth curves in Liyang chicken;2. The results of antibacterial spectra and growth curves of eleven strains with better antagonistic effect showed that: 4 strains isolated from NAC plate had wider range of antibacterial spectra,3 strains isolated from manganic nutrient agar plate had advantage on growth.

用点种法从大黄鱼肠道优势菌群中筛选出104株病原性副溶血弧菌的拮抗菌，进一步研究拮抗效果最好的11株拮抗菌的抗菌谱和生长曲线，其中NAC平板上筛选的4株拮抗菌的抗菌范围较广，锰营养琼脂培养基上筛选的3株拮抗菌具有生长优势。

The technological methods and optimal combination ratio were studied in the present paper, involving protection of natural color, elimination of bitter taste, mixtion and stability of the beverage. By single factor trial and orthogonal test, we optimized the processing techniques: Using 100mg/kg Zinc gluconate and 0.1% Calcium hydroxide as the color protection agent, burned in 80℃for 2min. The natural green color of material can be protected effectively.

通过单因素考察及正交实验设计确定了优化后的饮料制作工艺：以100mg/kg葡萄糖酸锌和0.1%氢氧化钙为护色剂，在漂烫温度80℃，漂烫时间2min条件下，有效地保持了苦瓜原料特有的绿色；以苦瓜皂苷为功能强化因子，以1.25%海藻酸钠为包埋剂，通过锐孔-凝固浴法对苦瓜原汁和苦瓜皂苷进行包埋；使用8%木糖醇、0.3%β-环状糊精、15%苦瓜汁和10%苦瓜胶囊对饮料进行调配，很好地保持了苦瓜特有的苦香味和口感；以0.15%黄原胶、0.2%琼脂、0.05%羧甲基纤维素钠复配方式，来增加饮料的稳定性。

Methods: The inhibitation of HL-60 cells by Rapamycin was measured by MTT assay. Morphic change of the apoptosis of HL-60 cells was observed by inverted microscope and light microscope, trapezoid straps by DNA agarose gel electrophoresis, and apoptosis rate by flow cytometry. Western-blot analysis was performed to detect the expression of Bax and Bcl-2 proteins after rapamycin treatment.

用MTT比色法测定雷帕霉素对HL-60细胞增殖的影响；用倒置显微镜和荧光显微镜观测细胞凋亡的形态学改变；DNA琼脂糖凝胶电泳观测凋亡的梯形条带；流式细胞仪检测细胞凋亡率；Western-blot检测HL-60细胞经雷帕霉素处理后，Bax、Bcl-2蛋白的表达情况。

Methods Based on molecular assembly technique, the matrix of agarose gel was deposited on the surface of glass plates,followed by drying, oxidation with NaIO 4 and cross linking with glutaraldehyde.

利用分子组装技术，以琼脂糖凝胶为基质，于玻片表面制成自组装膜，经过碘酸钠氧化后再与戊二醛分子偶联，而成为具有双功能分子层的薄膜。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。