琼脂

Suitable pharmaceutical carriers (e.g., Eupergit or Glyoxal agarose beads) are known in the art and vary according to the route of administration.

适合药学载体(如Eupergit或Glyoxal 琼脂珠)是本领域中已知的且根据给药途径改变。

Human umbilical venous endothelial cells were cultured, and were exposed to different concerntrations of HCY with/or not with CuSO4; Cell counting of detached cells were performed with a hematocytometer; the cell survival was monitored by MTT assay and the cellular injury was evaluated by LDH release; Hoechst 33258 staining was used to observe nuclear morphological changes and the apoptosis was measured by DNA agarose gel electrophoresis ;the percentage of apoptosis was quantified by flow cytometry.

体外培养人脐静脉血管内皮细胞，用不同浓度的HCY伴／不伴生理浓度Cu~(2+)与内皮细胞作用；细胞计数板检测脱落细胞率；MTT法及乳酸脱氢酶释放率的测定来衡量细胞的存活与损伤；Hoechst 33258荧光染色观察细胞核形态变化和DNA琼脂糖凝胶电泳检测细胞凋亡，并采用流式细胞术定量测定细胞凋亡率。

Results indicated that suppression of malignancy was observed in the hybrid cells, they became differentiated morphologically and functionally by:(1) changes in histochemical staining, nucleus-cytoplasm in ratio, and increase in cell size and nucleal heterochromation, but decrese in cell surface microvilli and number of nucleoli;(2) decrease of growth rate, mitotic index and DNA synthesis rate;(3) loss of the ability to growth in soft agar medium, and non-tumorigenic under heterotransplantation to nude mice;(4) reduction of chromosome number mainly of rat derivative and decrease in chromosomal aberration rate.

实验结果表明：（1）杂交细胞出现瘤细胞与网织红细胞杂交同样的表型逆转及去恶性化特征，主要表现在细胞体积增大、核质比例减小；DNA合同速率降低、分裂指数下降，细胞生长显著减慢；在软琼脂中不形成集落，在裸鼠体内失去了长瘤能力。

Methods The polyclonal antibody was obtained by immunizing rabbits with the crude extracts of Humulus pollen It was primarily identified using indirect ELISA and double immuno-diffusion.

用葎草花粉变应原浸液免疫兔，获得高效价抗葎草花粉蛋白抗血清，应用琼脂免疫双扩散、ELISA法以及Western blotting鉴定产生的抗体。

These indicates bioactive peptides in Chinese forest frog skins have great potential for the exploitation and utilization. S_(180) cells were inoculated hypodermically at the concentration of 1×10~6 cells per mouse and the bioactive peptides in Chinese forest frog skins were resolved into aseptically distilled water to make injection liquid. After injected continuously 15 days, the inhibition rate of tumor was calculated. The induction of tumor cell apoptosis of active peptides in Chinese forest frog skins could be detected by morphological

将S_(180)胃肉瘤细胞接种至小鼠皮下，选取中国林蛙皮肤生物活性肽不同浓度样品液，连续注射种瘤小鼠15天后，计算抑瘤率；采用透射电镜观察细胞形态学变化、DNA梯度琼脂糖凝胶电泳技术、MTT比色法、流式细胞检测等技术观察中国林蛙皮肤生物活性肽对K562肿瘤细胞凋亡过程的诱导。

Smith, and agar diffusion method was used to study the extraneous antibacterial activity with extraction from Illicium floridanum in boiling water and contrast with that of water.

用琼脂扩散法研究干莽草果实水提液的体外抗菌活性，并与清水组对照。

Smith, and agar diffusion method was used to study the extraneous antibacterial activity with extraction from Illicium floridanum in boiling water and contrast with that of water.

全草水提取物对常见致病菌的抑制作用方法用琼脂扩散法研究干莽草果实水提液的体外抗菌活性，并与清水组对照。

Zn~(2+), iminazole, EDTA, K+, Cu~(2+)could inhibit its activity respectively.

确立了核酸酶粗提物的琼脂糖凝胶电泳测活体系。

A jellylike substance used in styling hair.

任何一种用琼脂作胶凝剂的培养基。

A strain of breast tumor cell line ZHL2006 was established and biologically identified. The ZHL2006 cells grew fast in vitro and the population doubling time was 43.6h. Electronmicroscopical observation showed that there were microvilli on the surface of the cells, nucleus irregularity, karyoplasmic ratio increased, nucleolus distinctness, et al.. Chromosome number and structure were abnormal. Immunohistochemistry showed that PCK were expressed. The ZHL2006 cell line could form onto clone in soft agar and had oncogenicity in athymic mice.

ZHL2006细胞在体外生长迅速，群体倍增时间为43.6h；倒置相差显微镜及Giemsa染色镜下观察细胞为多边形及梭形；透射电镜观察可见细胞表面有微绒毛，核高度不规则，核浆比例增大，核仁明显等；染色体数目和结构异常；广谱细胞角蛋白染色阳性，说明其自上皮而非来自基质；同时ZHL2006细胞系在软琼脂中可形成克隆，具裸鼠致瘤性。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。