琼脂

METHODS Hp in gastric biopsy specimens from 259 patients with gastroenteric diseases was studied by bacterial culture method. In vitro , antimicrobial susceptibility of 50 strains Hp of random selection was tested by E test and agar dilution method.

方法采用细菌培养的方法对259例胃肠疾病患者胃粘膜活检标本进行了幽门螺杆菌的分离鉴定，并用琼脂稀释和浓度梯度法对随机抽取的50株幽门螺杆菌进行了抗生素敏感性试验。

The red alga Gelidium is used to make the gelatin-like product called agar .

红藻石花菜属被用来生产凝胶状的琼脂。

Extracted from a type of red algae (of the Gelidium and Gracilaria genera), AGAR is a gelling agent used in Japan since the 15th century.

提取的一种红藻（的Gelidium和江蓠属），琼脂是一种胶凝剂在日本使用，因为15世纪。

METHODS: Cytometry and soft agar were employed in observing the biological characters of MEC-1 and Mc3 cell strain. The expressions of the two sub-types of MEC-1 and Mc3 cell strain, SSTR1 and SSTR2, were detected by hybridization in situ. The condition of the binding between MEC-1, Mc3 and 125I-RC-160 was analyzed by radioactive genin binding analysis.

采用细胞计数法、软琼脂法等观察MEC-1及Mc3细胞株生物学特性；以原位杂交法检测MEC-1，Mc3细胞株SSTR1及SSTR2两种亚型的表达情况；以放射配基结合分析法分析MEC-1，Mc3细胞与125I-RC-60的结合情况。

The establishment of germfree plantlet: Used 1/2MS + agar + sucrose, the burgeoning rate was high and plant was strong.

结果表明：(1)无菌苗的建立：用1/2MS+琼脂＋蔗糖，出苗率高（90%上）、苗壮。

Methods:By means of germinative test and agar diffusion,130 sorts of Chinese herbal medicine were screened synchronously.

采用琼脂扩散法和芽管试验法对 1 30种中草药进行同步筛选。

Results:thrity-one drugs were sfited by germinative test,twenty-one by agar diffusion method,fourteen by both method.

结果 ：应用芽管试验法筛选的有效药物有 31种，应用琼脂扩散法筛选的有效药物有 2 1种，其中两种方法均有效的有 1 4种。

Germinative test ; Agar diffusion ; Drug screening ; Candida albicans

芽管试验；琼脂扩散；药物筛选；白假丝酵母

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

So in our test aantagonistic germ was isolated from ginger soil and was studied. 108 strains of germs were isolated by tube dilution assay from the soil of gingerin Qingmu town,Bazhong city. Q6 strain was screened from these strains of germsthough the first screened and rescreened double with deck agar method andimproved aperture-strikeing method.

本实验从巴中市青木镇堆放腐烂姜并且又长出健康姜苗的根际土中分离出106株细菌，在涂布有姜瘟病原菌青枯劳尔氏菌的肉汤培养基上进行初筛，得到6株对青枯劳尔氏菌有较强拮抗作用的细菌，通过摇瓶发酵后利用发酵液，采用改良的琼脂孔扩散实验复筛，获得一株具有强拮抗作用的菌株，命名为Q6号。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。