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Methods The minimal inhibitory concentrations of 134 clincal isolates of bacteria were determined by standard agar dilution method,and miconazole nitrate cremor.

采用琼脂平皿稀释法测定2%硝酸舍他康唑软膏对134株临床分离分离致病菌的最低抑菌浓度,并与硝酸咪康唑霜进行比较。

Objective To evaluate the in vitro antifuangal activity of 2% sertaconazole nitrate ointment and miconazole nitrate cremor. Methods The minimal inhibitory concentrations of 134 clincal isolates of bacteria were determined by standard agar dilution method,and miconazole nitrate cremor.

目的 评价硝酸舍他康唑软膏的体外抗菌活性方法采用琼脂平皿稀释法测定2%硝酸舍他康唑软膏对134株临床分离分离致病菌的最低抑菌浓度,并与硝酸咪康唑霜进行比较。

By means of the transmission electron microscope we found the typical morphology variation of apoptosis,such as the concentration and side-accumulation of the nuclear chromatin,the fragmentation of the nuclear and the concentration of the cytochylema etc.Cell cycle detected by flow cytometry showed one visible apoptosis peak (20.6%) in front of the peak of G1.The typical ladder was found in the 15 g.L-1 agarose gel electrophoresis of the nucleus DNA.

透射电镜下可见细胞核染色质浓缩、边集、核碎裂及胞质浓缩等凋亡细胞典型的形态学改变;流式细胞术周期分析显示在G1期峰前存在一个凋亡峰(20.6%);核琼脂糖凝胶(DNA 15 g.L-1)电泳呈梯状。

Dissolve the pancreatic digest of gelatin, the dibasic potassium phosphate, and the agar in the water, with warming, and allow to cool.

将白明胶胰酶消化物、磷酸氢二钾、琼脂溶解于水中,伴以加温,并静置放凉。

To identity the strains of Eimeria conserved in our laboratory are purebred,11 strains of 6 Eimeria species were separated by single-oocyst method and infected chicken of 1—5 days old.

为了建立鸡球虫纯株,选用1~5日龄雏鸡,采用琼脂块单卵囊分离法对实验室保存的6种11株鸡球虫进行纯化,并选用2周龄雏鸡,对每个虫种/株选取1个单卵囊分离物进行增殖和种类鉴定。

The plasmid DNA bands were detected clearly by agarose-EtBr gel electrophore sis.Two forms of plasmid DNA molecules,namely covalently closed circular DNA mol e-cules and open circular DNA molecules,were observed under electron microscope.

琼脂糖-溴化乙锭凝胶电泳分析在两株菌的DNA中鉴别出明显的质粒带,用电镜方法在两株菌的DNA制剂中均观察到共价闭合超螺旋和开放型环状两种构型的DNA分子。

Thus,CMG-KALA nanoparticles have great potential as gene vectors for transfection in vitro.2.GS-peptide nanoparticles were synthesized as previous reported method in order to improve the ability of cell penetrating,endosome escaping and nuclear targeting.

通过激光共聚焦、荧光光谱以及红外光谱证明KALA可以成功地连接到CMG纳米颗粒表面;通过静电引力吸附将DNA与CMG-KALA纳米颗粒进行复合,并通过琼脂糖凝胶电泳证明该纳米颗粒对DNA具有很好的保护作用。

The apoptosis was evaluated by AQ-stained fluorescin, DNA ladder by gel electrophoresis and subdiploidy by propidium iodide-stained flow cytometry. The level of lipid peroxide under KI treatment was determined by measuring the production of thiobarbituric acid reactive substances.

取健康的猪甲状腺细胞培养24h后,加入不同浓度的碘化钾,观察细胞的形态改变;琼脂糖凝胶电泳、流式细胞术分析细胞DNA的降解;MDA法测定细胞膜的脂质过氧化程度。

The proliferation and kinetics, cell cycle, clonalforming rate, and apoptosis of MEC- 1 cells, before and after FHIT gene transfection in vitro, and tumor loci formedon mice after injection of transferred MEC- 1 cells in vivo were observed by immunochemical staining, flow cytometryanalysis, and so on.

结果外源性FHIT基因转入黏液表皮样癌MEC- 1细胞后,S期细胞显著减少,G1期细胞增多;细胞倍增时间从(21.03±0.41)h延长为(26.86±0.71)h;软琼脂克隆形成率显著降低;细胞发生退行性改变和出现凋亡早期表现。

The effects of light, agar and carbon sources on the growth of in vitro cultured root tips of Fraxinus mandshurica were studied.

研究了光照有无、琼脂添加与否和不同碳源对离体培养的水曲柳根尖生长的影响。

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