琼脂

Five methods namely improved CTAB method, CTAB-silica extraction method, SDS method, low pH extraction method with high salt and Zhou shiliang method were adopted to extract genome DNA from the leaves of Citrus aurantium. And three methods including ultraviolet spectrophotometer, agarose gel electrophoresis and SSR-PCR amplification were used to detect extracted sample DNA.

以枳壳叶片为试验材料，分别采用改良CTAB法、CTAB-硅珠法、SDS法、高盐低pH值法和周世良法5种方法提取枳壳基因组DNA，并通过紫外分光光度计、琼脂糖凝胶电泳及SSR-PCR扩增3种方法对所提取的DNA样品进行检测。

Methods The Mφ functions in various groups of rats with scald on the 5th and 10th day after scald were respectively determined with methods of McAb APAAP, agar bacteriolytic plate and MTT colorimetry.

应用McAb APAAP桥联酶标法、琼脂溶菌板法和MTT比色法等技术，分别测定了各组烫伤大鼠不同时相腹腔Mφ各种功能的变化。

With sodium succinate as carbon source and bromothymol blue as pH indicator,20 BTB agar plate positive strains were isolated,from which four of them were identified as aerobic denitrifiers.

利用琥珀酸钠作为碳源，溴百里酚蓝作为pH指示剂，共筛选得到20株BTB琼脂平板阳性菌。通过反硝化性能测定，复筛得到4株好氧反硝化细菌。

All the isolates were first identified by different culture media including Niger seed agar. CACA agar, urease tests, ID32 identification kit, 37℃ growth test, and capability of forming capsule, and then the sequence of the LSUrDNA D1/D2 region were further analyzed.

分别以油菊籽、咖啡酸玉米琼脂、刀豆氨酸－甜菜碱－溴麝香草酚蓝培养基、尿素酶试验、37℃生长试验、ID32生化鉴定板和英膜形成试验等初步鉴定后，再经大亚基核糖体DNA的D1/D2区域序列分析进一步鉴定菌种。

The results Showed that twenty isolates have the following biological aspects:① Capability of forming capsule,② Growth at 37℃ within 3days,③ Forming brown colonies on Niger seed agar, CACA agar,④ Urea hydrolysis test positive within 96 hours.⑤ No blue colonies were found on CGB agar.⑥ The genus could be identified by ID32 identification kid, but the species could not.⑦ The sequence of the LSUrDNA D1/D2 region analyzed confirmed to be C. neoformans var neoformans.⑧ Serotype A and AD were found in these isolates, Serotype A was predominant(83.33%),⑨ Some biodiversities of isolates existed.

结果 20株菌皆具有以下特征：①形成英膜，②37℃3天内生长试验皆为阳性，③咖啡酸玉米琼脂和油菊籽培养基皆可以产生褐色菌落，④尿素酶试验96h皆呈阳性，⑤CGB培养基未见蓝色菌落生长，⑥ID32鉴定板可以鉴定到属，种的鉴定结果各不相同，⑦大亚基核糖体DNA的D1/D2区域序列分析证实皆为新生隐球菌的新生变种，⑧血清型仅见A和AD二型，以A型为多数(83.33%)，⑨20株临床分离株随机扩增DNA多态性分析有一定的生态多样性。

Effect of alternation of agar and agarose on the plant differentiation frequency of calli from wild rice.

琼脂浓度对水稻愈伤组织植株再生率和内源激素含量的影响。

Compared with fresh samples, dried with silica gel, the genomic DNA was extracted from Castanea henryi leaves, which preserved for 10 days, 2 months, 4 months, 6 months, 8 months, 10 months, 11 months, 12 months, 13 months, 14 months, 15 months, 16 months, 17 months, 18 months, by improved CTAB methods.

以野生锥栗幼叶为对照，采用硅胶干燥法，设置不同保存时间（10 d，2、4、6、8、10、11、12、13、14、15、16、17、18个月），对保存不同时间的锥栗叶样抽提基因组总DNA，利用紫外分光光度计及琼脂糖凝胶电泳检测提取DNA的浓度、纯度、得率及质量等指标，评价不同保存时间对基因组DNA的影响。

The hemolysin consists three components,B,L1,and L2(35.36.and 45kDa,respeotively),combinations of all three moieties produed the unique ring-shaped zone of hemolysis,increased rabbit vascuolar permeability,caused fluid accumulation in rabbit ileum loop and appeared cellulotoxic reaction on Vero cells.

溶血素由B、L1和L2 3种成份组成，分子量分别为35、36和45kDa 3种成份的协同作用在血琼脂上引起靶状溶血，使兔皮肤血管通透性增加，兔小肠肠段结扎产生积液反应，Vero细胞形态学改变。

The optimal determining conditions were obtained: adding quantity of a monolayer medium 15ml, the suspension concentration of Micrococcus luteus 109 cfu/ml, the adding quantity of Oxford Plate 100μl, agar concentration 1%, the concentration of Na2HPO4 1％, and the pH of the medium adjusted to 7.0. The medium was optimized by single factor and the orthogonal experiments on the basis of the centrifugate of distiller's grains. The optimal composition was obtained as follows: sucrose 0.6, peptone 0.1, yeast extract 0.2, K2HPO4?

以管碟法为基础，对影响Nisin效价测定的各种主要因素进行了分析，并得出了Nisin效价测定的最佳条件：采用单层培养基（每个平板加量为15ml），检测培养基中Na2HPO4·12H2O浓度改为1％，菌悬液浓度109cfu/ml，不加吐温20，琼脂浓度1％，培养基pH7.0，牛津杯加液量100μl ，30℃培养24小时。

METHODS: The Chelerythrine was used as the experimental group with concentrations ranging from 24.4μg/ml to 390.6μg/ml prepared with BHI broth medium with contained 2% glucose, and BHI culture medium was used as the control group.

采用二倍稀释法，用含2%蔗糖的脑心浸萃液肉汤琼脂液体培养基，将白屈菜红碱稀释为从390.6μg/ml到24。

My first article for Screen Power Magazine, which is to this day is the official magazine of Jackie Chan, was a DVD review of the Hong Kong actioner Gen-X Cops, starring Nicholas Tse and Daniel Wu.

我的第一篇文章为屏幕力量杂志，在对这天是Jackie Chan 正式杂志，是香港actioner Gen X 警察的DVD 回顾，担任主角Nicholas Tse 和丹尼尔吴。

His lips grazed hirs, Now what are you going to do?

他的唇使吃草了 hirs，现在你将要做什麼？