琼脂

The transformed cell could be agglutinated by lower concentration of ConA and could grow in semisolid agar.

转化细胞可被低浓度ConA凝集，并在软琼脂内生长。

In order to study the protection of β-carotene and vitamin C against the transformation of human lung fibroblasts cell induced by nickel sesquioxide, HLF cells were treated repeatedly by different concentrations of Ni2O3 in vitro.β-carotene (5.4mg/L) and vitamin C(1.8mg/L) were added into the media containing Ni2O3 (1.0mg/L) respectively. The identification of malignancy of the transformation of HLF cell was carried out by the tests of ConA and the growth on semisolid agar culture. The results showed that Ni2O3 could induce the malignant transformation of HLF cell. The transformed cell proliferated rapidly.

为了探讨三氧化二镍(Ni2O3)诱导人肺成纤维细胞恶性转化作用以及β-胡萝卜素和维生素C的保护作用，用不同浓度的Ni2O3在体外多次处理HLF细胞，利用刀豆凝集素A凝集实验和软琼脂集落形成实验进行转化细胞的恶性鉴定；同时添加β-胡萝卜素(5.4mg/L)和维生素C的食物(1.8mg/L)，观察对Ni2O3转化作用的影响。

The antitumor effects of sesquiterpene lactones scabertopin and isodexyelephantopin from the whole plant of Elephantopus scaber were studied in vitro on SMMC-7721,Hela and Caco-2 cancer cell lines.

采用四甲基偶氮唑盐比色法和琼脂糖凝胶电泳法等研究地胆草倍半萜内酯化合物scabertopin(1)和isodeoxyelephantopin(2)在体外对SMMC-7721、Hela和Caco-2三种肿瘤细胞增殖的影响。

The inocula of oocysts were collected from the droppings by purifying with Sheather's sugar flotation method. Two days-old chickens were inoculated by purified inocula of 10,000 oocysts, and vaccinated with one dose of MDV (CVI988 strain) simultaneously.

籍由Sheather's sugar flotation的方法所纯化隐孢子虫的成熟卵囊，经品接种每只10，000卵囊於1日龄之小鸡，并同时皮下接种1剂量的MD疫苗(CV1988株)，於接种后第7天和第12天抽血分离血清，与得自MD病鸡羽毛囊的MD病毒沉降抗原作用，经由琼脂沉？

ShTat1.3 and ShTat1.5 are two variable antigen types of a cloning Anhui strain of Trypanosoma evansi.Total RNA of the two variable antigen types were extracted by acid guanidinium thiocyanate-phenol-chlorform single step method.

应用异硫氰酸胍一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1.3和ShTat1.5总RNA，在含有甲醛的琼脂糖凝胶电泳后，转印到硝酸纤维膜上。

The spores of Cheilolejeunea ventricosa X.L.He were cultured on MS media (without acid and vitamin, pH=6.2, with 1% agar and 1% sucrose). The sporeling development and oil body ontogeny were observed. After a 55-day-long axenic culture, young plants were obtained successfully. Sodium hypochlorite solution was used to sterilize the capsules. Our results revealed that the sterilizing treatment with 0.1% Sodium hypochlorite solution for a period of 5 seconds was optimal. Oil bodies in primary protonemata are nearly homogeneous, but distinctly compound in the leaf cells of the primary leafy shoots.

首次研究了膨叶唇鳞苔的孢子萌发、原丝体和油体发育及配子体发生的过程，结果表明：(1)使用经过改良的MS培养基（不加氨基酸和维生素，pH值为6.2，琼脂和蔗糖浓度均为1%）能在55 d左右的时间利用孢子培养获得植物体；(2)用0.1%的次氯酸钠溶液，消毒5 s是消毒孢子的最佳方案；(3)油体在孢子萌发初期为均一型，到幼叶发生后，成为可见的聚合型，但聚合的程度比野生材料要弱。

The only way to diagnose this as sporotrichosis is to culture Sporothrix schenckii

幸运的是应用抗生素沙保弱琼脂在室温下并不难培养出该菌。

Establishment of a bovine mammary tissue culture system Bovine mammary tissue was cultured in superfusion system or stationary system, and the influence of these two methods in the activity and ultrastructure of tissue was investigated according to LDH vigor, trypan blue dying, agrose gel electrophoresis, transmission microscope observation.

1奶牛乳腺组织体外培养方法的建立采用组织灌流和组织块贴壁法体外培养奶牛乳腺组织，通过LDH活力测定、台盼兰染色、琼脂糖凝胶电泳、透射电镜技术比较两种培养方法对奶牛乳腺组织活性及组织超微结构的影响，建立奶牛乳腺组织的培养体系。

Methods:The susceptibilities of 80 gonococcal epidemic strains to 8 fluoroquinolones were detected by agar dilution methods .

临床标本分离的 80株淋病流行株，采用琼脂稀释法测定淋球菌对 8种氟喹诺酮药物的最小抑菌浓度及交叉耐药情况。

Drug susceptivity is measured for minimal inhibitory concentration by agar plate dilution method.

药敏试验用琼脂平板稀释法检测MIC，按NCCLs规定的标准进行。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。