琼脂

The article studied on processing technology of tomato beverage using tomato as material, they did experiment aim to increase juice yield , stability of the beverge and to keep colour. The results indicated juice yield is able to reach 81.5% after preheating, adding pectase , pressure filtering, the processing technology improved the utilization of tomato.

以番茄为原料进行饮料的加工，针对提高番茄出汁率、饮料稳定性、饮料色泽的保持工艺研究实验，结果发现，对番茄预热后，再加果胶酶处理、加压过滤工艺可使番茄的出汁率达到81.5%，提高了番茄的利用率；番茄饮料中添加2‰，质量比5：2：3的羧甲基纤维素钠、黄原胶、琼脂复配增稠剂可使饮料在常温保存三个月仍具有良好的稳定性。

Put forward the viewpoint of denoting the rigidity of media by peptonization intensity(g/cm2).Ascertained that the peptonization intensity of initial media is 160-170 g/cm2,that of subculture and rooting media is 150~160 g/cm2.4 Experiment on sugar source of media.

培养基琼脂用量计算的依据通过试验，提出用胨力强度(g/cm~2)表示培养基硬度的观点，并确定了初代培养基胨力强度为160～170g/cm~2，继代培养基、生根培养基均为150～160g/cm~2。

Methods: The author chose randomly sixty healthy children as the control group and one hundred and sixty children with asthma in acute period before ICS therapy as the experimental group. The experimental group were treated with ICS therapy and then eighty-nine children of this group were followed up for 3 months ,sixty-eight for 6 months and sixty for 12 months. The ICS in the study was budesonide with the trade name as Pulmicort. We adopted swab from the pharynges respectively, inoculated it in agar plate, isolated the bacteria, and evaluated the distribution and variation of the microbial population in pharyngeal portion.

方法随机选择健康儿童60例和支气管哮喘急性发作期患儿160例采取其口咽拭子，并于哮喘患儿在吸入型糖皮质激素(布地奈德，商品名普米克)治疗后分别随访3个月(89例)、6个月(68例)、12个月(60例)分别采取其口咽拭子，接种于琼脂平板，分离细菌，按程序鉴定，观察其咽部菌群的分布及其演变。

The photosensitization effects of trisruthenium- on DNA caused by visible light has been studied by using of agarose electrophoresis.

采用琼脂糖凝胶电泳研究了以可见光引发的三联吡啶钌与DNA之间的光敏反应。

The sensitivity of capillary electrophoresis was about 750 times higher than that of the agarose gel electrophoresis, which can only detect the 1/3 of the total PCR product of 74 picogram of template DNA (as amount of DNA in 13 cells).

毛细管电泳检测微量目的基因扩增产物的综合灵敏度是琼脂糖凝胶电泳的750倍。

The PCR products were detected by both agarose gel electrophoresis and capillary electrophoresis in order to compare their sensitivity. Results Capillary electrophoresis detected the 1/600 of the PCR product from 20 picogram of male DNA, as amount of DNA in 3-4 cells.

结果 毛细管电泳可检测到20 pg男性模板DNA（相当于3-4个细胞中的DNA含量）PCR扩增产物总量的1/600，琼脂糖凝胶电泳可检测到74 pg男性模板DNA（相当于13个细胞中的DNA含量）PCR扩增产物总量的1/3。

The routine diagnostic methods for GPMV were the Agargel precipitin assays, Serum-neutralization test , have been established. These methods have played important role in control of GPMV, but which has lower sensitivity and take longer time.

国内外学者先后建立了琼脂扩散试验、中和试验、血凝抑制试验等血清学诊断方法，在一定程度上为防治鹅副粘病毒病发挥了重要作用，但同时也存在灵敏度差、操作烦琐、检测所需时间长等不足，已不适应更有效地控制该病的需要。

The results showed that Non-haemagglutinating activity was showed in the isolate. In agar diffusion test,there was a clear precipitin band formation to anti-IBDV specific serum. 28-day-old chicks inoculated with the isolate showed disease,and the isolate was recovered from the tissues of these chickens. The 1 041bp specific fragment were amplified by RT-PCR with sequence-specific primers based on IBDV VP3 gene. The VP3 gene shared 97.7%~98.2% nucleotide identities to very virulent IBDV,97.7%~95.2% nucleotide identities with classical strains from seqiemce analysis.

结果表明，该分离病毒无血凝性，在琼脂扩散试验中能与抗IBDV特异性血清出现1条清晰的白色沉淀线；人工感染28日龄雏鸡出现与临床一致的病变，并回收到病毒；应用针对IBDVVP3基因的特异性引物进行RT-PCR，能扩增到长度为1041bp的特异性目的片段；序列分析发现分离毒VP3基因与IBDV超强毒和经典毒株的核苷酸同源性分别为97.7%~98.2%和95.2%。

Marked changes of apoptosis including condensation of chromatin and nuclear fragmentation were observed very clearly by Hoechst 33258 fluorescence staining and a characteristic "ladder" of DNA fragments was elicited by agarose gel electrophoresis; Western blot analysis revealed that caspase-3 was activated by the loss of caspase-3 proenzyme (32kDa) and the appearance of its 20kDa subunit, and that along with the apoptotic process caspase-3 activity was increased concurrently.

Hoechst 33258荧光染色观察到典型的核浓缩、核碎裂等细胞凋亡的形态学变化，琼脂糖凝胶电泳观察到细胞凋亡时的DNA"梯形"条带；Western blot检测结果表明32kDa的caspase-3酶原被激活，出现20kDa的亚单位活化片段，同时在细胞凋亡过程中，caspase-3活性显著增高。

Objective:To study proteinuria with agarose gel zone electrophoresis.

目的 ：运用琼脂糖凝胶区带电泳进行蛋白尿的分析。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。