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Glycolate oxidase (EC 1. 1. 3. 1, GO) was purified by 137. 4 fold from leaves of Brassica campestris L. ssp. chinensis var. utilis and the specific activity attained 130.9 U/mg. protein. Chromatography of purified GO through Sephadex G-200 showed two symmetric peaks of both protein and activity, which overlapped fully with each other. Further with electrophoresis on agrose plate, a protein spot and an activity spot were detected in electrophoretogram after respective staining, and the spot of protein covered that of activity, which indicated that the purified enzyme was of a high quality.

从菜心叶片中纯化了乙醇酸氧化酶,比活性达130.9 U/mg,纯化倍数为137.4倍;纯化酶经Sephadex G-200凝胶过滤显现单一而对称的蛋白吸收峰和活性峰,且两者完全重叠;进一步经pH 8.3缓冲系统的琼脂糖平板电泳、染色,各在负极端出现一个活性斑点和蛋白染色斑点,且两者的位置完全对应;表明纯化的菜心叶片乙醇酸氧化酶已达到较高的纯度。

Hematin was immobilized on glassy carbon electrode surface by agarose hydrogel.

用循环伏安法研究了血红素-琼脂糖凝胶修饰电极在水和有机溶剂/水混合溶液中的电化学和催化还原过氧化物的性质。

Using modern biology research technique and means,plasmid extract and agarose gel electrophoresis of molecular biology level explore test were carried out for coal with not coal department the hereditary property of Thiobacillus ferrooxidans.

利用现代生物学研究方法和手段,对煤系与非煤系氧化亚铁硫杆菌的遗传特性进行了质粒抽提和琼脂糖凝胶电泳等分子生物学水平的基础性探索试验,对于质粒在硫杆菌中普遍存在的观点提出了质疑。

The growth patterns of hircine non-antral follicles cultured in the an agar gel culture system resembled in vivo.

在三维琼脂凝胶培养体系中,山羊无腔卵泡的生长模式类似于体内。

The orange osmanthus skin-traditional Chinese medicine being taken as raw material, the active ingredient-phenol was extracted with the method of steam distillation and then the derivative of paeonol-aminoethanol and 2,4-dinitrophenyl hydrazone was synthesized. The structural characterization of the derivatives carried out was identified and the test in the anti-bacterial role of three kinds of gram-negative and positive bacteria was done with the method of agar diffusion.

方法]以丹皮为原料,用水蒸气蒸馏法从中药丹皮中提取活性成分丹皮酚,进而合成丹皮酚缩乙醇胺和2,4-二硝基苯腙衍生物,对衍生物进行了结构表征,并采用琼脂扩散法,对3种革兰氏阴性菌、阳性菌进行了抑菌性试验。

The spectrophotometric method examination resultindicated, withdraws sample DNA rate in, OD260/280 value in;Agarose the gelatin electricity swims the examination result to indicate,molecular weight size about 23kb; Withdraws sample DNA may use EcoRIand the HindIII restrictive internally tangent enzyme carries on cuts.

分光光度法检测的结果表明,提取的样品DNA得率在20ug/g-80ug/g之间,OD260/280值在1.9-2.0之间;琼脂糖凝胶电泳检测的结果表明,分子量大小在23kb左右;提取的样品DNA可以用EcoRI和H indIII限制性内切酶进行切割。

Jia. A new gel casting of ceramic by reaction of sodium alginate and calcium iodate at increased temperatures .

琼脂糖凝胶大分子在陶瓷原位凝固成型中的应用,1999,27(1):16~21

Cytotoxicity ; Agar diffusion method ; Cosmetic ; Irritancy

细胞毒;琼脂扩散法;化妆品;刺激性

In order to evaluate the irritancy of cosmetic ingredients and products, we used the cell culture technique improved the agar diffusion method to study the eytotoxic effect of SLS, the standard skin irritant, on L-929 cells.

为了评价化妆品原料和产品的刺激性,利用细胞培养技术,改良了琼脂扩散法,较为系统地研究了皮肤标准刺激物十二烷基硫酸钠对L-929细胞的毒性作用,并就这一体外方法与体内皮肤毒理学实验方法的相关性进行了研究。

Be like,the Mu Si that make is the most important is jelly raw material pink of agaragar, isinglass, jelly pink, there also is special Mu Si white now.

制作慕斯最重要的是胶冻原料如琼脂。鱼胶粉。果冻粉等,现在也有专门的慕斯粉了。

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Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在,所有的孩子都要在综合学校继续学习,所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地,使人们普遍认识到发展不能以牺牲农业为代价。

Detailed Intrinsic Reaction Coordination calculations were carried out to guarantee the optimized transition-state structures being connected to the related tautomers.

同时,做了详尽的内禀反应坐标计算,以保证所得到的过渡态连接相应的始末异构体。