琼脂

The first type is the chaining colony, between the colony does not have the obvious demarcation line, these colonies are work as when agar-agar and experiment mix, a zoogloea is dispersed the result; The second type is the water film type colony which forms between the agar-agar and the plate bottom; The third kind is the water film type colony which forms in the plate edge or the agar-agar surface.

第一种类型是链状菌落，菌落之间没有明显界线，这些菌落是当琼脂和试验物混合时，一个细菌块被分散所致；第二种类型是在琼脂和平皿底之间形成的水膜样菌落；第三种是在平皿边缘或琼脂表面形成的水膜样菌落。

The company introduces the overseas equipment's new technology unceasingly, the development produces the automatic sand which, mechanic Taiwan, dentistry multi-purpose powder dust catcher dentistry mechanical devices and so on mechanic dresser, agar-agar heater, agar-agar mixer compares favorably with the overseas similar products, the company present has become one of oral cavity most influential enterprises.

公司不断引进国外设备的新技术，开发生产出与国外同类产品相媲美的自动喷砂机、技工台、牙科多功能粉末集尘器技工打磨机、琼脂加热器、琼脂搅拌机等牙科机械设备，公司目前已成为口腔界最具影响力的企业之一。

Antagonistic strains of rhizobacteria against the fungi of Rhizoctonia solani, the causative agent of the damping-off disease of mungbean, were selected on potato dextrose agar, tryptic soy agar and malt yeast glucose agar with 62 rhizobacteria isolated from the rhizosphere of mungbean respectively.

采用对峙法分别在马铃薯葡萄糖琼脂，胰酶大豆琼脂和麦芽酵母葡萄糖琼脂3种培养基上测定了从绿豆根际分离的62株细菌对于绿豆立枯病菌的拮抗作用，筛选出2株具有较强拮抗作用，即05-33和05-49。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

Results: The colonies of gonococcus growing on ordinary nutrient agar and yeast extract agar were smaller than 2 mm, while those growing on yeast extract blood agar and chocolate agar were 2～3 mm and the recovery rate was 26% higher.

结果，淋球菌在普通营养琼脂和酵母浸膏琼脂上生长的菌落均小于2mm；在酵母浸膏血琼脂和巧克力琼脂上生长的菌落为2～3mm，计数回收率高出26％。

The results showed that callus started forming at root segments from buds of potato cultivar: Ingush No.5 and Ingush No.8 in greenhouse cultured 45d after placement on culture medium: MS+2,4-D 2mg/L+BAP 3mg/L+NAA 0.5mg/L+sucrose 30g/L+agar 7g/L and diffused light.

结果表明，青薯5号和青薯8号温室生长幼芽根组织接种MS+2，4-D 2mg/L+BAP 3mg/L+NAA 0.5mg/L+蔗糖30g/L+琼脂7g/L培养基上散光条件下培养45~90d产生愈伤组织，诱导率为0.024%；青薯5号愈伤组织接种1/2MS+2，4-D 0.5mg/L+BAP 2mg/L+KT 2mg/L+GA30.5mg/L+ZT0.2mg/L+蔗糖20g/L+琼脂7g/L培养基上培养80d分化出再生植株，分化率为6.25%，再生植株切成一叶茎段接种于MS+BAP 0.1mg/L+IAA 10mg/L+GA30.1mg/L+白糖30g/L+琼脂6g/L培养基上培养3周长成株高8~10 cm、叶7~10片、叶色深绿和根系发达的小植株。

Methods: Agar gel immunodiffusion test was conducted twice in agar gel plate with 0.1 mg/L bromophenol blue, first, agar gel immunodiffusion test was carried out with AFP-HSP70 cross-linking protein and AFP antibody, then the test was conducted again with the processed precipitation band and HSP70 antibody.

在含0.1 mg/L溴酚蓝的凝胶琼脂板上，进行2次琼脂免疫扩散实验，第1次是用AFP-HSP70交联蛋白和AFP抗体进行琼脂免疫扩散，把扩散后沉淀带处理后再次和HSP70抗体进行琼脂免疫扩散。

The results showed that the growth of root tips in liquid medium was better than that in agar-solidified medium and solid-liquid two phase medium, for that increasing agar concentrations in medium will reduce the root growth; darkness was beneficial for Fraxinus mandshurica root tip culture in vitro; as the carbon sources, sucrose was better than glucose and maltose as the carbon ...

结果表明，不添加琼脂的液体培养基对根尖的生长较添加琼脂的固体培养基和固—液培养基好，原因是增加琼脂的量会抑制根的生长；暗培养比光培养更有利于水曲柳离体根尖的培养；蔗糖作为碳源的效果较果糖和麦芽糖好，其中以3%的蔗糖对离体根尖的生长效果最好。

Effects of Cgtophaga spp.,normal temperature and low-alkali treatment s on the yield and quality of agar from Gracilaria tenuistipitata

阐述食纤维粘菌处理与常温低碱处理从海南细基江蓠中提取琼脂的研究，探讨它们在琼脂提取中的作用和不同条件下对琼脂凝胶强度与出胶率的影响。

The strain can normal growth using agar as the sole carbon source medium and after the growth of 4-5 days, agar massive was hydrolysised with the appearance of a great amount of holes.

该菌株在琼脂作为唯一碳源的培养基中能够正常生长，并在4-5天的生长后，琼脂上产生大量的水解坑，在培养15天左右整个琼脂都被液化。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷