球蛋白

Gold nanoparticles in size of 10 nm were prepared by the improved method of trisodium citrate, and were used to label goat anti-human immunoglobulinM to obtain a resonance scattering spectral probe for IgM.

用粒径为10 nm的金纳米微粒标记羊抗人免疫球蛋白M，制备了IgM的免疫纳米金共振散射光谱探针。

Response: A greatly simplified and condensed version is this; Dr. Henske found that LAM is caused by a loss of the protein, tuberin.

尽量简单地说就是这样的；Henske博士发现：LAM是由于缺失了一种叫做马铃薯球蛋白的蛋白质而引起的。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Observe the formula's effects to the diabetic nephropathy rat model cause by small dose STZ injured, high lipin feed and hemiresection, the results showed that can decrease significantly blood sugar after diet 2h, control availably hyperplasia of glomerulus mesenteric cell, inhibite tumefaction of glomerulus capillary endothelial cell, keep integrality of podocytic process and basement membrane, relieve vasal cavum stricture, reduce the GFR and excretory rate of 〓-MG, improve the status of high blood lipin, specially decrease significantly TC, reduce the 〓 and T/K, improve the status of microcirculation obstacle, elevate the activity of 〓-〓-ATP enzyme.

观察降糖益肾方对小剂量STZ诱导并单侧肾切除，高脂饲料喂养的糖尿病肾病大鼠模型的影响实验结果：对2h后血糖有显著降低作用（p.05），能有效抑制肾小球系膜基质的增生，减轻肾小球毛细血管内皮细胞肿胀，保持足突与基底膜的完整性，减轻血管腔狭窄，降低GFR、尿微量白蛋白以及〓-微球蛋白的排泄率（p.05），能改善高血脂状态，降低血TG、TC含量（p.05），降低〓与T／K比值（p.05），改善微血管循环障碍的状态；能升高肾组织中〓-〓-ATP酶的活性（p.05）。

The blood sample of carotid was drawn out in 24 rabbits 30 minutes after veinal injection of xinmaining and prothrombin time was recorded.

结果 ：与对照组比较，不同浓度的心脉宁可延长凝血时间和凝血酶原时间，并缩短优球蛋白溶解时间。

IgA pemphigus is a newly characterized group of autoimmune intraepidermal blistering diseases presenting as vesiculopustular eruptions with a predilection in axillary and groin areas.

免疫球蛋白A天疮是一种新归类的自体免疫皮肤水性疾病。其临床表现为搔痒的水性与脓性皮疹，好发位置为身体对摩的部位。

But children born to mothers who are highly viremic for HBV (HBeAg-positive) have a 32% chance of becoming infected during birth, despite prophylactic vaccination and hepatitis?B immunoglobulin.

但是HBV母亲且有高度毒血症者所生的小孩，即使有预防性注射疫苗与B型肝炎免疫球蛋白，仍有32%机会在生产时发生感染。

To attain more comprehensive understanding of flounder immune system, it is necessary to characterise and localise lymphoid cells within the different lymphoid organs. Use polyclonal antibody or monoclonal antibody as a tool will benefit this kind of study.

制备针对免疫球蛋白的多克隆或单克隆抗体，作为研究工具，对免疫器官中产生抗体的组织和细胞进行定位，可以了解免疫器官在防御疾病过程中是如何发挥作用的，以便对应用疫苗奠定理论基础。

Unlike "solid-state" frequency converters which convert your incoming 50Hz or 60Hz AC power to DC then chop it back up to output a synthesized AC sine wave, our TEMCo Frequency Converter MG Sets produce a sine wave in exactly the same way your utility generates it.

与&固态&变频器转换您接收50Hz或60Hz的交流电源，直流然后砍它备份输出合成正弦波交流电，我们坦科球蛋白集变频器产生正弦波绝对相同的方式产生的效用它。

The lectin agglutinated rabbit, mouse, chicken and human ABO blood cell, and the activity of this lec...

该凝集素能凝集兔、小鼠、鸡、人A、B、O红细胞，其凝集作用可被猪甲状腺球蛋白明显抑制。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。