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These shoots were examined by PCR,PCR-Southem hybridization and DNA dot blotting.Results indicated that the geneencoding A1aB1b subunit of glycinin has been actually introduced into mulberry.

用PCR、PCR-Southem及DNA斑点杂交等方法对这些Km抗性苗进行了鉴定,结果证实大豆球蛋白基因确已被导入桑树体内。

The upstream regulatory region of the storage protein was separated from Glycine max variety "Ji Nong T7018" genomic DNA, according to published glycinin 11S G7 subunit (Gy7) gene(GenBank AF319776) sequence, a pair of primers was desgeined, in order to amplify the array before Gy7 gene initiation codon.

我们以大豆品种&吉农T7018&的基因组DNA为模板,根据已发表11S球蛋白G7亚基(Gy7)基因(GenBankAF319776)序列,设计一对引物,通过PCR扩增得到Gy7基因的启动子序列。

To understand the mechanism of mucosal immune response, indirect immunostaining with Staphylococal protein A-Horseradish peroxidase was employed for the study of distribution, change of quantity and rule of increasing or reducing of immunoglobulin A-secreting cells in Harderian Gland, providing theoretical basis for prevention and control of avian disease.

本研究采用改良硫酸铵盐析法、Macro-prep High Q阴离子交换柱层析纯化得到鸡分泌型免疫球蛋白A,免疫家免制备兔抗鸡SIgA多克隆抗体。

objective:to approach the immunity status of pulse magnetic field treatment on treating paediatric lobular pneumonia.method:120 paediatric cases,suffering from lobular pneumonia by pulse magnetic field treatment,admitted by our department during the period of january 2008 and october 2008,designing observation group;120 cases,which were health check-up the same term,designing control group.observation group were picked blood 2ml before treatment and after treatment 4 weeks;control group was picked blood 2ml during health check-up.blood serum was separated and immunoglobulin was inspected by immunity nephelometry and immunity function were analyzed.results: iga igm igg value in 120 cases observation group was lower than of the control group and iga igm igg value was obviously heightener after treatment.the difference between the two cases was significant in statistics(p.05).conclusion: pulse magnetic field treatment on treating paediatric lobular pneumonia improve immunity function,boost up resistance of paediatric cases.

目的:探讨脉冲磁疗治疗小儿支气管肺炎前后体液免疫功能状况。方法:我科于2008年1月~2008年10月收治的部分支气管肺炎患儿采用脉冲磁疗治疗120例,设为观察组;同期门诊健康体检儿童120例,设为对照组,观察组于治疗前及治疗后4周分别采血2ml,对照组于体检时采血2ml,分离血清用免疫比浊法进行免疫球蛋白检测,并对治疗前后患儿的体液免疫功能进行分析研究。结果:120例观察组患儿治疗前iga igm igg值均低于正常对照组,且观察组治疗后iga igm igg值均较治疗前明显增高,且经统计学处理差异均有显著性(p.05)。结论:脉冲磁疗治疗小儿支气管肺炎改善了患儿的体液免疫功能,从根本上增强了抗病能力。

To investigate the clonal rearrangements of immunoglobulin heavy chain gene and T-cell receptor γ gene in peripheral blood cells of patients with hemapoietic malignancies and their significance, the clonal rearrangements of IgH and TCRγ genes were detected by polymerase chain reaction in peripheral blood cells of patients with hemapoietic malignancies, including 32 cases of non-Hodgkin lymphoma, 18 cases of acute myeloid leukemia, 24 cases of multiple myeloma, 8 cases of acute lymphoblastic leukemia and 6 cases of chronic lymphoblastic leukemia.

检测各种血液系统肿瘤患者外周血细胞免疫球蛋白重链基因和T细胞受体γ基因克隆性重排并探讨其意义。通过多聚酶链式反应方法检测32例非霍奇金淋巴瘤、18例急性髓性白血病、24例多发性骨髓瘤、8例急性淋巴细胞白血病及6例慢性淋巴细胞白血病患者外周血细胞IgH及TCRγ克隆性基因重排。

Methods:Thyroid function,thyroid microsome antibody,and thyroid globulin antibody were determined with radioimmunoassay in 23 CRF complicated by primary hypothyroidism patients,and the serum calcium,phosphorus,potassium, cholesterol, glucose, urea nitrogen, hemoglobulin,and endogenous creatinine clearance rate were also assayed.

用放射免疫法测定23例CRF合并甲减患者的甲状腺功能、甲状腺微粒体抗体、甲状腺球蛋白抗体及检测血清钙、血清磷、血清钾、血清胆固醇、血清肌酐、血糖、尿素氮、血红蛋白、内生肌酐清除率并与31例单纯CRF患者对照进行分析。

Methods The clinical data of splenectomy for the treatment of children with hepatolenticular degeneration complicated with hypersplenism were analyzed retrospectively. The comparison was made on the changes of blood cell and pre- and post-opertive immunoglobulin.

回顾分析30例小儿肝豆状核变性合并脾功能亢进而行脾切除的临床资料,对手术前后血细胞和免疫球蛋白的变化进行比较。

The diagnosis of histiocytic/dendritic cell sarcomas is currently based on morphology and the presence of immunophenotypic features of H/DC differentiation.

现在对组织细胞/树突状细胞肉瘤的诊断,都是依据形态特征和H/DC分化特征的免疫表型。H/DC肉瘤是否发生了克隆性免疫球蛋白受体基因的重排,此问题争论了几十年。

Objective To study the clinical value of the change of β2-MG, EGF and TGF-β1 in children with congenital hydronephrosis.

目的 探讨先天性肾积水患儿β2-微球蛋白(β2-MG)、表皮生长因子及转化生长因子-β1 (TGF-β1)含量变化的临床意义。

Selection criteria: Hypothyroidism with the increased thyroid microsomal antibodies, thyroid peroxides antibody, or thyroglobalin antibody which detected in city, and provincial hospital in gestation or before pregnancy were diagnosis as Hashimotos thyroiditis. Hyperthyroidism supported with the thyroid ultrasonography or ECT scans or confirmed by fine-needle-aspirate biopsy of the thyroid, excluded the hyperfunctioning thyroid adenoma and multinodular goiter, were diagnosis as Graves disease.

纳入标准:对妊娠前或妊娠期间曾在省、市级医院检查有甲状腺功能减退且伴有抗微粒体抗体或抗甲状腺过氧化物酶抗体、抗甲状腺球蛋白抗体增高者为慢性淋巴细胞性甲状腺炎孕母;有甲状腺功能亢进且甲状腺B超或甲状腺ECT扫描或甲状腺细针穿刺病理检查证实、并排除高功能腺瘤及多发性结节者为GraveS病孕母。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。