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球蛋白

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Lactoglobulin was purified from lyophilized whey with Sephacryl S 200 gel filtration after casein removed from skimmed milk by adjusting pH to its isoelectric point.

用调pH至等电点的方法将脱脂牛乳中的酪蛋白除去,乳清用SephacrylS-200凝胶过滤,得到较纯的β-乳球蛋白

The results showed that human transferrin could be expressed in these cell lines and mouse or goat mammary gland following transfection, and its expression level could be improved dramatically when it was driven by rabbit enhancer and promotor as compared with goat beta lactoglobulin promotor. Transgenic mice were generated by either microinjection or lentivirus infection.

结果显示所有表达载体均可指导人转铁蛋白在细胞以及小鼠和山羊乳腺中表达,兔转铁蛋白增强子与启动子组合可显著性增加人转铁蛋白的表达水平,且其指导表达的效率明显高于山羊β乳球蛋白启动子。

P and orally to male BN rats for consecutive 4 weeks every 7 days. In different intervals, blood samples obtained from the tail of rat were used for determination β-lactoglobulin specific IgE level by ELISA.

采用连续灌胃及腹腔注射致敏蛋白的方式致敏BN大鼠,ELISA测定β乳球蛋白特异性IgE水平。

A regulatory element specific for a milk protein is essential for generating mammary bioreactors. This study cloned and sequenced a 1. 59kb DNA fragment derived from bovine β—lactoglobulin gene.

为了制备乳腺生物反应器所需要的乳腺特异性表达的调控序列,本文用PCR法克隆了牛β—乳球蛋白基因的一个1.59kb的片段,它包括0.65kb的5′侧翼序列,第一、二外显子和第一内含子。

Methods Regulation region of bovine β-lactoglobulin geneand full-length genomic DNA encoding hEPO were used to construct eukaryotic expression vector,and the transgenic goats were produced by pronuclear microinjection.

方法采用牛β-乳球蛋白基因调控元件和hEPO全长编码序列基因组DNA构建真核表达载体,应用受精卵原核注射的方法制备hEPO转基因山羊。

The most component of Whey proteins in ruminant milk isβ-lactoglobulin. The regulatory elements of BLG have been used in construction of mammary gland specific-expressed vector.

球蛋白(β-lactoglobulin,BLG)是反刍动物乳中含量最高的乳清蛋白,其调控元件已被用于乳腺特异表达载体的构建。

The results showed as follows: Five bands in SDS-PAGE, lactoferrin, bovine serum albulin, immunoglobulin G,β-lactoglobulin and α-lactoalbumin were detected.

结果表明:从SDS-PAGE电泳图谱上可见有5个清晰的条带,自上而下分别为乳铁蛋白、牛血清白蛋白、IgG重链、β-乳球蛋白和α-乳白蛋白。

To investigate the hydration of proteins in magnetized water, pure water was treated with a static magnetic field over different effective treatment time t(subscript eff. Viscosimetry and oxygen nuclear magnetic resonance (17O-NMR) spectra were recorded. The hydration properties of β-lactoglobulin in magnetized water were examined by differential scanning calorimetry and NMR.

为探讨蛋白质在磁化水中的水合作用,首先利用粘度测定及氧17核磁共振(17O-NMR)对经静磁场处理不同有效时间t(下标 eff后的纯水进行了分析,进一步又利用差示扫描热量计及NMR对溶解于磁化水的尽乳球蛋白的水合特性进行了分析。

Significant level of β-lactoglobulin was detected in the secretions 12d to 1d prior to parturition, Activities of γ-GT, NAGase, AKP in the secretions were generally higher than that of colostrum and milk, while lactoperoxidase activity was lower than that of milk.

在分娩前12d~1d的样品中均检测到β-乳球蛋白。分娩前乳腺分泌物中γ-GT、NAGase、AKP活力一般显著高于初乳和常乳,而LP活力显著低于常乳。

The use of Rh immune globulin to prevent the disease in babies of Rh negative mothers has become standard practice, and the disease, which used to claim the lives of 10,000 babies each year in the US alone, has been virtually eradicated in the developed world. In 1980 Drs. Pollack, Gorman, Freda, and Finn shared the Albert Lasker Award for their work on Rh disease.

使用Rh免疫球蛋白来预防在Rh阴性血型的母亲的胎儿的疾病,成为一种常规做法,这种曾经仅在美国每年夺走10,000名婴儿生命的疾病,在发达国家几乎被摧毁了。1980年,博士波拉克,戈尔曼,弗里达,芬恩由于他们在Rh疾病上的工作分享了亚伯雷斯克奖。

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