球蛋白

Aim: To study the amino acid-composition of purified 3 major components(arachin, conarachin I and conarachin II) and the five purified polypeptides. To separate the methionine-rich proteins and analyze their changes of synthesis and degradation during development and germination in peanut seeds.

目的：分析花生球蛋白、伴花生球蛋白Ⅰ和伴花生球蛋白Ⅱ及其组成多肽的氨基酸组成，筛选高甲硫氨酸多肽并研究其在花生种子发育和萌发过程中的合成降解规律。

The polymerization of mono-substrate food proteins such as sodium caseinate, bovine serum albumin, glycinin and β-conglycinin,β-lactoglobulin and α-lactoalbumin by MTGase were studied by SDS-PAGE combined with the technique of imaging.

采用SDS-PAGE结合凝胶成像技术研究了还原状态下MTGase催化酪蛋白酸钠、牛血清蛋白、大豆球蛋白和β-伴豆球蛋白(β-conglycinin)、β-乳球蛋白和α-乳白蛋白等单底物蛋白聚合，结果表明：MTGase较易催化CN和BSA聚合，其次为glycinin，而β-伴豆球蛋白、β-LG和α-LA最不易。

Results showed that casein could be completely removed of by adjusting pH 4.6 and centrifuging at 1100g.β-lactoglobulin was absorbed by the cation-exchange resin eluented by phosphate buffer. Through limiting the quantity of acid whey, the purity concentration percent of immunoglobulin was above 80% determined by SDS-PAGE. The ratio of activity of immunoglobulin losing decreased in simulated pepsin and the residual immunoglobulin content percentage was about 80% in 60 minutes.

实验结果表明：调节脱脂牛初乳pH=4.6采用1100g低速离心即可达到完全去除酪蛋白的良好效果；利用含有磷酸根基团的缓冲液处理阳离子树脂柱会专一吸附酸性乳清中的β—乳球蛋白，达到分离乳清中免疫球蛋白与β—乳球蛋白的良好效果，控制乳清样品处理量可使免疫球蛋白纯度达到80%以上；微胶囊化的免疫球蛋白可以延缓胃液对免疫球蛋白的消化分解，减缓免疫球蛋白活性损失速率，在60分钟内仍可保留80%的活性。

Purification of mlckfrom rabbit skeletal muscle 肌球蛋白轻链激酶...used by:Stull Lab,University of Texas, Southwestern Medical Center

肌球蛋白轻链激酶( myosin light chain kinase，mlck )具有激酶活性和非激酶活性，在平滑肌收缩过程中起着关键酶调控的作用。

The clinic of nursing care have documented that large dose injection of gamma globulin can prevent and cure the complication of aeteria coronaria , large dose intravenous drip of gamma globulin,2g/kg,intravenous drip slowly in 8 to 12 hours, gamma globulin has two therapeutic actions including immune vicariousness and immune accommodation. It can improve the patients' IgG level of blood quickly after intravenous drip of gamma globulin.

护理临床已证明大剂量丙种球蛋白注射，可有效防治川崎病时冠状动脉并发症，大剂量丙种球蛋白静脉滴注，2g/kg，于8-12h缓慢输入，丙种球蛋白具有免疫替代和免疫调节双重治疗作用，经静脉输入后，能迅速提高患儿血液中的免疫球蛋白

This papersummarizes the studies on glycinin including components,structure and gene families.The homogeneity of the glycinin gene sequences was compared by bioinformatics.Heredity Distances of the six glycinin genes were analyzed. Special emphasis is laidupon comparing the sequences and components of amino acids in the glycinin. Thecontents of major amino acids in the intact glycinin and in the acid and basic peptideswere analyzed. In view of others and our researches, we advance some new thinkingand tactics of improving rice nutritional quality by using glycinin genes.

本文在简要介绍大豆球蛋白组分、结构及基因家族后，采用生物信息学方法分析比较了不同种类大豆球蛋白基因之间的序列同源性，并对不同大豆球蛋白基因的遗传距离作了分析；本文还重点比较分析了不同大豆球蛋白的氨基酸序列和组成，以及不同酸性肽和碱性肽中重要氨基酸的含量，基于对前人工作的总结和我们的分析结果，本文提出了利用大豆球蛋白基因进行水稻营养品质改良研究的新思路和新策略。

The Sulfhydryl content of glycinin and β-conglycinin are increased with the increasing of pressure and prolong of processed time; Fluorescence analysis reveals that glycinin has larger hydrophobic regions while processed by higher pressure; But to β-conglycinin, the hydrophobic regions change according to different pressure; The changes of ultraviolet difference spectra of pressured glycinin show that amino acid residues which have ultraviolet absorbance are exposed on the surface of the protein molecule with its unfolding by high pressure denaturation as well as the exposure of sulfhydryl and dissulfide groups.

处理压力的升高和时间的延长都将使大豆豆球蛋白和β-伴大豆球蛋白的半胱氨酸残基暴露，巯基含量增加。荧光分析表明，经压力处理后大豆豆球蛋白表面疏水区域增多：而对于β-球蛋白，在低压作用下，其疏水区域减少，在高压作用下，其疏水区域增加，但在过高的压力下，β-伴球蛋白中的疏水区域又呈现减少的趋势。

Soybean antigen protein ; 7S globin ; 11S globin ; glycinin ;β-conglycinin

大豆抗原蛋白； 7S球蛋白； 11S球蛋白；大豆球蛋白；β-伴大豆球蛋白

The results showed that the cadmium pollution increases the contents of total protein and globulin,and decreases the ratio of albumin to globulin.The effect of cadmium on albumin is not statistically significant.The threshold of effect of cadmium on sero proteins of Tilapia mossambica is 0 005mg/L.

试验结果表明，镉能升高血清总蛋白、球蛋白含量，降低白蛋白／球蛋白比值，但对血清白蛋白含量没有影响；镉对血清球蛋白各组分的影响有其特异性；对莫桑比克罗非鱼血清球蛋白成分产生作用的镉浓度阈值为0.005mg／L。

Western blots were performed to investigate the cross-reactivity of various labeled antibodies with serum proteins from other fish species. Results Immunoglobulins of five fish species were purified, including bighead carp, carp, argus snakehead fish, ricefield eel, sea bass. The titers of five rabbit anti-sera obtained up to 1:32 examined by agarose double immunodiffusion. Five kinds of purified rabbit anti-fish immunoglobulins antibodies were labeled with HRP and their activities were up to 1:10000 examined by EUSA. Four kinds of rabbit anti-fish immunoglobulins antibodies labeled with HRP could react with serum proteins from other fish species.

结果 纯化了鳙、鲤、乌鳢、黄鳝、鲈五种鱼血清免疫球蛋白，免疫双扩散法测定兔抗这五种鱼免疫球蛋白的抗血清效价均达到1：32，并对纯化的兔抗鳙、鲤、乌鳢、黄鳝、鲈五种鱼类免疫球蛋白的抗体进行了HRP标记，ELISA测定标记抗体的效价达到1：10000左右，Western blots显示标记抗体与部分其他鱼类免疫球蛋白之间存在不同程度的交叉反应。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。