球虫的

One hundred and eighty 14-days-old chickens were divided randomly into coccidia primary infection group (I1),coccidia secondary infection group (I2),and control group.Immune indexes were examined by using immune staphytococcal protein A rosette test,indirect enzyme-linked immunosorbent assay,cellular culture technique and MTT method.

将180只14日龄雏鸡随机分为毒害艾美球虫初次感染组、二次感染组和对照组，应用免疫SPA菌体花环、间接ELISA及细胞培养技术和四甲基偶氮唑盐测定法对相关免疫学指标进行了检测，以研究毒害艾美球虫二次感染对雏鸡外周血液免疫功能变化的影响。

Coccidiosis is a major parasitic disease in poultry and caused by several species of Eimeria that infect the gut of chickens.

鸡球虫病是由艾美耳球虫感染鸡引起的一种危害极其严重的寄生原虫病。

Coccidiosis in chickens ,which shows a series of intestinal symptoms caused by Coccidiosis Eimeria,is a serious protozoan disease in the world.

鸡球虫病是一种由艾美耳属球虫引起的鸡肠道疾病，也是危害极为严重的全球性寄生虫病。

Travel-associated infections can include giardiasis, cryptosporidiosis, amebiasis, isoporiasis, strongyloidiasis, and schistosomiasis.

与旅行相关的感染包括贾第虫病、隐孢子虫病、阿米巴病、等孢球虫病、类圆线虫病和血吸虫病。

Piglet spore ball worm disease depend on by pig spore ball worm for a living in nursing period and wean piglet leather cell one parasitics protozoon's disease caused at the small intestine recently, present world distribution, cause the serious economic losses to pig farm .

仔猪等孢球虫病是由猪等孢球虫寄生在哺乳期及新近断奶仔猪小肠上皮细胞所引起的一种寄生性原虫病，呈世界性分布，给养猪场造成严重的经济损失。

The coccidiosis is an autoecious disease which is rapid in infection and powerful in harm. The outbreak is widespread. It is a dominating disease in the intensived chicken faming.

球虫病是一类寄生性原虫病，传染性强，危害性大，尤其在养鸡业中，鸡球虫病的发生十分普遍，是集约化养鸡业中最主要的疾病。

Chicken coccidiosis,one of the important diseases,severely endangers poultry industry.Its pathogens belong to Eimeria.

鸡球虫病是危害养禽业的重大疫病之一，其病原属于艾美耳属的球虫，世界上公认有7种。

Result The nested PCR assay was specific and there is no cross-reaction with other parasites, such as Eimeria spp., Cryptosporidium spp., Giardia sp., Toxoplasma sp., Trichuris sp. and cattle ciliate. The assay was able to detect as low as 2.85 × 10^(-2) fg of control positive DNA. The results of detection of clinical samples revealed that the assay was coinciding with microscopic examination.

该套式PCR能特异性地扩增出牛源环孢子虫基因组DNA中相应片段，与艾美耳球虫、隐孢子虫、贾第虫等10种相关原虫基因组DNA无交叉反应；最低能检测到2.85×10^(-2)fg的阳性参照DNA；对临床样品检测结果表明PCR技术检查阳性者显微镜检查都为阳性。

In order to study the characteristics of humoral immunity on E. tenella, specific vitelline antibody of E.

为了探讨体液免疫在鸡球虫病中的作用，通过制备柔嫩艾美尔球虫E。

The plasmid pcDNA3.1-3-1E and pcDNA3.1 was respectively used to immunize SPF chicks by injection intra-muscularly in thigh muscle at 14 and 21 days of age.Chicks in each group except that in the unchallenged control group were challenged with 5×104 E.acervulina sporulated oocysts at 28 days post-inoculation,and the immune protective efficacy was observed.

将构建的重组质粒以及空载体质粒分别于14、21日龄分2次经腿部肌肉注射免疫SPF雏鸡，28日龄除非免疫非感染对照组外各组攻击性感染5×104个堆形艾美球虫孢子化卵囊，观察真核表达质粒对球虫感染鸡的免疫保护作用并探讨其免疫保护机理。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。