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Real-time fluorescence quantitative PCR technique has been widely applied, such as mRNA expression, detections of DNA copy number and determination of mononucleotide polymorphism, as well as in the clinical medicine including accurate quantitative examination of mycobacterium tuberculosis, Type B and Type C hepatitis, AIDS virus, gonococcus, and chlamydia trachomatis.

实时荧光定量PCR的应用范围非常广泛,包括mRNA表达的研究、DNA拷贝数的检测、单核苷酸多态性的测定等,已广泛应用于医学临床,它能对结核分枝杆菌、乙型、丙型肝炎、爱滋病病毒、淋球菌、沙眼衣原体等病原体进行准确的定量检测。

Incorporation of nisin in the coating was effective in inhibiting Micrococcus flavus, and a-tocopherol incorporation retarded lipid oxidation in the model emulsion and in the milk cream.

在禁止微球菌 flavus 方面涂料的 nisin 的结合是有效的,和一- tocopherol 样板的乳状液和牛奶乳酪的智能迟滞的脂质氧化的结合。

As vinegar to the streptococcus, pneumococcus, Staphylococcus albus and others have a definite bactericidal effect, if the gauze wipe with vinegar drenched body odor affected area, then 4 ~ 5 hours there will be no smell; heating the water to be soaked in vinegar, can be treated tinea pedis and other diseases caused by mold.

至于醋对链球菌,肺炎球菌,白色葡萄球菌等都有一定的杀菌作用,如果醋的纱布擦拭身体受影响地区的湿透的气味,然后4?5小时就没有异味,加热水浸泡在醋,可以治疗足癣和霉菌引起的其他疾病。

Their geographical origins are global Hamelin Pool,SharkBay,Australia;Alpine salt,Austria;Qingdao beach,China;saltern in Cadiz,Southern Spain;salted fish from North American seawater.

这些盐球菌属的来源分别是:澳大利亚的哈美林池和沙克湾,奥地利的阿尔卑斯盐场,中国的青岛盐场,西班牙南部的卡迪斯盐田,北美洲海水中的咸鱼。

A review of 113 bacteriologically proven cases of meningococcemia at the Royal Children's Hospital from 1990 to 2003, confirmed the prognostic value of these criteria of DIC ( disseminated infravascular coagulation),so accurate that those cases with more than three of these features present were associated with a very high mortality.

通过回顾1990~2003年间皇家儿童医院的113例为细菌学所证明的脑膜炎球菌血症之后,证实了关于弥漫性血管下凝血的诊断指标的预后价值。这些指标是如此准确,以致符合其中三项以上者均伴有较高的死亡率。

At the root canal therapy process, hidden in the root canal isthmus, bifurcation area, triangle, shape and irregular dentinal tubules within the Enterococcus faecalis prepared by mechanical means it is difficult to remove, it can be biofilm, proton pump tolerance mechanisms such as root canals harsh ecological environment, to avoid conventional drugs, the role of disinfection, resulting in the failure of root canal therapy.

在根管治疗过程中,隐藏在根管峡部、分叉区、三角区、形态不规则区及牙本质小管内的粪肠球菌难以用机械预备的方法去除,它可通过生物膜、质子泵等机制耐受根管内苛刻的生态环境,躲避常规消毒药物的作用,从而导致根管治疗的失败。

The cells of isolate (CW2) stain Gram positively, the morphology of the cells of isolate was changed from rod to round in the process of growth, the cells in the initial phase of logarithmic growth were long rod, majority of cells singly existed, minority of cells were geminated or formed a long discontinuous chain, until to invariable phage of growth, the cells changed to globe.

分离出的菌株CW2为革兰氏阳性,在生长过程中细菌细胞的形态有球杆的变化,在对数生长期的初期细胞是直杆或者弯杆状,大多数单生,少数成对或者成不连续的链状,到恒定期变成类球形细胞。

The cells of isolate CW 2 stain Gram positively, the morphology of the cells of isolate was c hanged from rod to round in the process of growth, the cells in the initial phase of logarithmic growth were longrod, majority of cells singly existed, minority of cells were geminated or formed a long discontinuous chain, until to invariable phage of growth, the c ells changed to globe.

分离出的菌株CW2为革兰氏阳性,在生长过程中细菌细胞的形态有球杆的变化,在对数生长期的初期细胞是直杆或者弯杆状,大多数单生,少数成对或者成不连续的链状,到恒定期变成类球形细胞。

This method conformed the following conditions: spleen phosphodiesterase and micrococcal nulcease for digesting reagent and the concentration, temperature and digesting time of the two enzymes;the components of p32-post label alleviant and labeling time;the concentration of p1 nuclease; the concentration of T4 polynucleotide kinase for catalysing and labeling reagent;the components of bidirectional spreader and the spreading time ,etc.

确定了采用脾磷酸二酯酶和微球菌核酸酶消化底物以及两种酶的使用浓度、温度及消化时间;确定了P32标记缓冲液的主要成分及标记时间;确定了P1核酸酶的浓度;确定了T4多核苷酸激酶催化标记底物的浓度;确定了双向展开剂的主要成分及展开时间等。

DISCUSSION The copper response has been deeply studied so far with two bacteria, E. coli and Enterococcus hirae (33, 37, 39), but the elucidation of the copper systems in M. xanthus will provide a missing piece in the complicated puzzle of bacterial adaptation to this metal under natural environmental conditions: how do cells handle different copper concentrations during a complete life cycle?

讨论 铜的反应一直深入研究,所以到目前为止,有两名细菌,大肠杆菌和肠球菌平江( 33 , 37 , 39 ),但澄清铜系统米细菌将提供一个一块失踪,在纷繁复杂的拼图细菌适应这下金属的自然环境条件:怎么处理细胞不同铜浓度,在一个完整的生命周期?

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。