球菌的

Results The positive detecting rate of aerobe bottle against aerobe was 100%, Sensitivity was 10 CFU/ml. The positive detecting of anaerobe bottle against anaerobe was 100%, Sensitivity against peptococcus niger, Peptostreptococcus anerobius, bacteroides fragilis was 10 CFU/ml, 100 CFU/ml, 1000 CFU/ml, respectively.

结果 需氧瓶对需氧菌的阳性检测率为100%，检测敏感性为10 CFU/ml；厌氧瓶对厌氧菌的阳性检测率为100%，对黑色消化球菌、厌氧消化链球菌和脆弱类杆菌的检测敏感性分别为10 CFU/ml、100 CFU/ml和1 000 CFU/ml；厌氧瓶对兼性厌氧菌的阳性检测率为100%，检测敏感性为10 CFU/ml。

Results The results showed that the average concentration of the bacteria, fungi and total microbes in the outdoor environment were 5.04×10^4, 3.75×10^3 and 5.41×10^4 cfu/m^3 respectively and the fungi accounted for 6.92% of the total. The preliminary identification showed that the dominant bacteria mainly were Micrococcus, Bacillus, Pseudomonas and Staphylococcus and the average percentage were 37.3%, 21.0%, 10.3% and 9.5% respectively. Of the five fungi the dominant ones were Aspergillum, Penicillium, Mucor and Rhizopu. The average percentage were 42.0%, 36.0%, 4.4% and 4.2% respectively. Conclusion The outdoor air of Hefei has been polluted by airborne microbes.

结果 合肥城区空气细菌总数均值为5.04×10^4cfu/立方公尺，真菌总数均值为3.75×10^3cfu/立方公尺，合计为5.41×10^4 cfu/立方公尺，其中真菌占6.92%；对不同采样点的空气细菌和真菌进行了初步鉴定，优势细菌属为微球菌属、芽孢杆菌属、假单胞菌属和葡萄球菌属，分别占总细菌的37.3%，21.0%，10.3%，9.5%；真菌共检出5属，其中优势菌属为曲霉属、青霉属、毛霉属、根霉属，分别占总真菌的42.0%，36.0 %，4.4%，4.2%。

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

For observing the resistance of clinically isolated gonococcus and the standard bacterial strain to chemical disinfectants, standard strain with relatively high resistance was selected to carry out organic interfering substance test.

观察临床分离的淋球菌和标准菌株对化学消毒剂的抗力，选择抗力较高的标准菌株作有机干扰物试验。

A strain designated S7 which was isolated from the traditional dairy products was obtained using the agar-diffusion method employing Micrococcus flavus as an indicating strain.

以从传统乳制品中分离得到的7株乳酸菌为出发菌株，应用琼脂扩散法，以黄色微球菌为指示菌，筛选到了一株具有较大抑菌作用的乳酸菌。

DianSha is the third generation of carbostyril with a far stronger antibacterial activity than any other drug of the type. Its effective spectrum covers Gram-negative bacilli and staphylococcus aureus, especially effective on enterococcus.

典沙作为第三代喹诺酮类药物，其抗菌活性强于同代其它品种，对临床分离的大多数致病菌包括革兰氏阴性杆菌及阳性球菌均有较好的抗菌作用尤其对肠杆菌的作用最强。

To deepen the results, researchers from Rhodococcus erythropolis LSSE8-1 and Gordon's LSSEJ-1 strain in the successful expansion of desulfurization have been related to the gene fragments, and the sequencing is being carried out desulfurization projects microbial bacteria work to build .

为深化成果，研究人员从红平红球菌LSSE8－1和戈登氏菌LSSEJ－1中成功扩增得到了脱硫相关基因的片断，并进行了序列测定，目前正展开微生物脱硫工程菌的构建工作。

The main bacteria coloniesin the biofilm are methanosaeta and methanosarcina. According to the literature, sarcina is oneof the methanogen that can produce methane in high performance. The remaining of sarcina willapparently improve methane production.

根据以往的资料显示，八叠球菌是产气较好的甲烷菌之一，这种菌的驻留会使整个产气效果明显提高。

All C.neoformans contain serine protease; Serine proteinase perturbs the integrity of BMEC of the blood-brain barrier, improves open the level of BBB; Secreted serine protease from pathogenic fungi were virulence.

发现新生隐球菌分泌具有活性的胞外蛋白水解酶并含有丝氨酸蛋白酶成分；新生隐球菌自身分泌的丝氨酸蛋白酶可以干扰构成血脑屏障主要成分内皮细胞的完整性使得血脑屏障开放程度提高；致病性真菌分泌的丝氨酸蛋白酶可能是一种毒性因子。

Cephalexin is effective against a wide range of Gram positive and Gram negative bacteria including: Staphylococci, Streptococcus spp., Pneumococci, E. Coli, Klebslella spp, and Salmonella spp.

头孢氨苄是有效的针对范围广泛的革兰氏阳性和革兰氏阴性菌包括：金黄色葡萄球菌，链球菌属。，肺炎球菌，大肠杆菌，Klebslella菌和沙门氏菌。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。