球菌

Seven major bacterial isolates were obtained and purified. Their 16S rRNA genes were amplified by PCR for sequencing and their identities were determined with homology comparisons. Five of the seven isolates belong to Actinomycetales including Kocuria KD139, Rhodococcus KD140, Rhodococcus KD142, Arthrobacter KD230, and Arthrobacter KD232; one is classified as Bacillus d KD178; and another one as Stenotrophomonas KD237. The phylogenetic tree was also constructed based on the analysis of 16S rRNA gene sequences.

通过对分离菌株的16S rRNA基因序列进行分析，发现其中5株细菌分别属于放线菌目的考克氏菌属(KD139)、红球菌属(KD140和KD142)和节杆菌属(KD230和KD232)， 1株细菌属于杆菌目的芽胞杆菌d属(KD178)，另外1株细菌属于黄色单孢菌目的寡食单胞菌属(KD237)；同时我们构建了系统进化树，确定分离菌株的相对进化地位。

Aboriginal effective bacteria in the aerobic compost are Bacillus, Pseudomonas,Sporolactobacillus, Micrococcus, Enterobacteriaceae, Cellulomonas, Proteobacteria, Edwardsiellaand so on.

4禽粪便好氧堆肥过程中土著高效细菌分属芽孢杆菌、假单胞菌、芽孢乳杆菌、微球菌、肠细菌、纤维单胞菌、变形菌、爱德华氏菌等菌属。

Coli, Shigella sonnei s, Proteus, typhoid and vice Salmonella typhi, Pseudomonas aeruginosa, Vibrio cholerae and other enteric pathogens are effective; its ethanol extract on the number of gram-positive and-negative bacteria, and human-type Mycobacterium significant antibacterial activity; ebony decoction on in vitro to be sore dermatophytes, Epidermophyton floccosum, plaster-like pathogenic microorganisms in the skin of small spores of fungi

广谱抗菌：实验证明，乌梅水煎液对炭疽杆菌、白喉和类白喉杆菌、葡萄球菌、肺炎球菌等皆有抑制作用，对大肠杆菌、宋内氏痢疾杆菌、变形杆菌、伤寒和副伤寒杆菌、绿脓杆菌、霍乱弧菌等肠内致病菌也有效；其乙醇浸液对一些革兰阳性和阴性细菌，及人型结核杆菌均有显著抗菌作用；乌梅水煎液在试管内对须疮癣菌、絮状表皮癣菌、石膏样小芽孢菌等致病皮肤真菌有抑制作用

TBA analysis showed 0.01% of TP had evident antioxidation on lipids from fish meat, and TBA increased with the TP concentration accretion presenting an unlinear relation. The effects of antioxidation of 0.03% and 0.07% of TP were close, thus, the best choice is 0.03% in practical application.5. 93 bacteria strains were isolated and categorized into several spoilage bacteria classes: Aeromonas, Vibrio, Pseudomonas, Enterobacteriaceae, Flavobacterium, Acinetobacter, Photobacteriaceae, Brevibacterium, and Micrococcus. In the early stage of storage (1～3 days), Gram-positive bacteria were the major strains and accounted for 70%, most of them belonged to Brevibacterium and Micrococcus.

TBA试验结果显示，添加00.1%以上茶多酚对草鱼脂肪都有明显的抗氧化作用，随浓度增加TBA值增加，但不呈线性关系，0.03%～0.07%抗氧化效果比较接近，从经济、有效的角度考虑在实际应用中可采用0.03%的茶多酚。5、从草鱼中分离出93株菌株，鉴定出以下几类主要腐败细菌：气单胞菌属、弧菌属、假单胞菌属、肠道杆菌科、黄杆菌属、邻单胞菌属、发光杆菌属、产碱杆菌属、短杆菌属、微球菌属、不动杆菌属、无色菌属。

Results:The results showed that rate of the drug fast is very high. As the bacteria distribution ,the main pathogenic bacteria was the gram-negative bacilli(65.33 %), the second pathogenic bacteria was the gram-positive cocci(18.29%). and the mycetes(16.38%) occupied the third. In gram-negative bacilli the most were Pseudomonas aeruginosa,Acinetobacter baumannii,Klebsiella pneumoniae and Escherichia coli;Staph is the main pathogenic bacteria in gram-positive cocci;and in mycetes the main pathogenic bacteria were Candida albicans and Candida glabrate.

结果：EICU医院感染病原菌多为耐药菌，病原菌以革兰阴性杆菌为主占65.33％、革兰阳性球菌第二占18.29％、真菌第三占16.38％；革兰阴性杆菌中以铜绿假单胞菌、鲍氏不动杆菌、肺炎克雷伯菌及大肠埃希菌为主；革兰阳性菌主要以葡萄球菌属为主；真菌以白假丝酵母、光滑假丝酵母菌为主。

Com-pound 11 showed best antifungal activity with broad antifungal spectrum and proved to be more active against Cryptococcus neo-formans, C andida albicans, Microsporum lanosum and Trichophyton rubrum than ketoconazole.

化合物11的抗菌谱最广，抗真菌活性最高，对新型隐球菌、白色念珠菌、羊毛状小孢子菌和红色毛癣菌的抗菌活性比酮康唑高，有进一步开发的价值。

Among pathogens , the most common pathogens associated with VAP were Pseudomonas aeruginosa ( 3013 %), Acinetobacter baumanii (1115 %), Klebsiella pneumoniae ( 1017 %), Escherichia coli ( 716 %) and S taphylococcus aureus ( 618 %). Their multiple drug2resistance to antibiotics was serious. Drug sensitivity tests indicated that the GNB had lower drug2 resistance to such antibiotics as imipenem , amikacin , piperacillin/ tazobactam , ceftazidime , cefepime , cefoperazone/sulbactam , ciprofloxacin and ticarcillin/ clavulanate. Polymicrobial pathogens were more isolated in late2onset VAP than in early2onset VAP.

结果 共检出致病菌383 株，革兰阴性细菌占8411 %，其中非发菌占5114 %，肠杆菌科占3216 %，革兰阳性球菌占1218 %，其中MRSA和MRSE 占615 %，真菌占311 %；前5 位致病菌分别为铜绿假单胞菌(3013 %)、鲍氏不动杆菌(1115 %)、肺炎克雷伯菌(1017 %)、大肠埃希菌(716 %)、金黄色葡萄球菌(618 %)等；药敏结果显示这几类菌株的多重耐药现象严重，较为敏感的抗菌药物有亚胺培南/西司他丁、阿米卡星、哌拉西林/他唑巴坦、头孢他啶、头孢吡肟、头孢哌酮/舒巴坦、环丙沙星及替卡西林/克拉维酸等，革兰阳性细菌均对万古霉素敏感；迟发性VAP 的混合培养比例显著高于早发性VAP。

Results showed that the specific activity of nitrile hydratase per dry cell weight in engineered Rhodococcus strains driven by Ptac, Pami-1, Pami-2 and PlacZ was 7.5, 6.3, 5.3 and 1.8 times of that in the wild, respectively. It indicated that these promoters could be well recognized by RNA polymerase of Rhodococcus.

结果表明，启动子Ptac、Pami-1(7 bp SD-ATG间隔)、Pami-2(13 bp SD-ATG间隔)和PlacZ在紫红红球菌ATCC 33278中启动表达腈水合酶的比酶活分别是野生菌株的7.5、6.3、5.3和1.8倍，表明这些启动子都可以被红球菌的RNA聚合酶所成功识别。

85 And 119486/79. They are a novel type of antibiotic having both the wide antibacterial spectrum and high safety of penicillin and cephem antibiotics belonging to beta -lactam antibiotics, as well as the potent antibacterial activity and high beta -lactamase stability of carbapenem antibiotics.Sodium-(5R, 6S)-6-[-1-hydroxyethyl]-7-oxo-3- [-2-tetrahydrofuryl]-4-thia-1-azabicyclo [3.2.0] hept-2-ene-2-carboxylate 5/2 hydrate (faropenem sodium, hereinafter referred to as compound 1) is currently used as an oral drug for various infectious diseases and is reported to show potent antibacterial activity against not only methicillin-sensitive Staphylococcus aureus, Streptococcus pyrogenes and Streptococcus pneumoniae but also gram-positive bacteria for which conventional beta -lactam drugs have proved ineffective such as penicillin-resistant pneumococci, oral staphylococci and enterococci, also showing a wide antibacterial spectrum covering gram-negative bacteria such as Haemophilus influenzae and anaerobic bacteria such as the genus Bacteroides, which activity is due to its novel skeleton penem ring (Kagaku Ryoho no Ryoiki The Field of Chemotherapy, Vol.

他们是一种新型的抗生素都具有广泛的抗菌谱和高度的安全青霉素和cephem抗生素，属于β-内酰胺类抗生素，以及作为强大的抗菌活性和高β-内酰胺酶稳定的碳青霉烯类antibiotics.sodium -（ 5 R ， 6 S ）-6 -[ - 1 -羟乙基] - 7 -氧- 3 - [ - 2 - t etrahydrofuryl] - 4 -硫杂- 1 -氮杂双环[ 3 。2.0]庚- 2 -节能- 2 -羧酸5 / 2水合物（法罗培南钠，以下简称为化合物1 ）是目前用来作为口服药物，为各种传染病和报道，以显示强大的抗菌活性，不仅对甲氧敏感的金黄色葡萄球菌，链球菌pyrogenes和肺炎链球菌，但也克阳性菌为常规β-内酰胺类药物已证明无效，如青霉素耐药pneumococci ，口腔葡萄球菌和肠球菌，也显示出广泛的抗菌谱，包括革兰阴性菌如流感嗜血杆菌和厌氧菌如属杆菌，这活动是由于其新颖的骨架青霉烯环（（化学ryoho没有ryoiki领域的化疗），第13卷，第10号，第74-80 ， 1997年）。

The results indicated: COD removal efficiency could remain at 70%, the product gas was mainly composed of methane, carbon dioxide and hydrogen, tile maximal methane producing activity sequentially were 211 mLCH4gVSS^(-1)d^(-1), 314 mLCH4gVSS^(-1)d^(-1), 319 mLCH4gVSS^(-1)d^(-1); The main components of effluent were acetic acid and propanoic acid; The dominant microorganisms sequentially were Bacillus, Coccus, Methanothrix and Methanosaet.

实验结果表明：COD的去除率可维持在70%左右，产气主要含有甲烷、二氧化碳和氢气，每格最大产甲烷活性依次为211 mLCH4gVSS^(-1)d^(-1)，314mLCH4gVSS^(-1)d^(-1)，319mLCH4gVSS^(-1)d^(-1)；出水产物为乙酸和丙酸；每格优势菌种依次为杆菌、球菌、甲烷丝状菌和甲烷八叠球菌。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。